Construct: ORF ccsbBroad304_03442

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003670.1_s304c1, BRDN0000462361
Derived from:
ccsbBroadEn_03442
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
INO80 (54617)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03442
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54617 INO80 INO80 complex ATPase subunit NM_017553.3 100% 100%
2 human 54617 INO80 INO80 complex ATPase subunit XM_011521685.3 76.8% 75.5% (many diffs)
3 human 54617 INO80 INO80 complex ATPase subunit NR_104038.1 71.4% 1_317del;3589_3590ins128;4858_6225del
4 human 54617 INO80 INO80 complex ATPase subunit XM_011521686.3 58.2% 57.6% 0_1ins1807;32_33ins143
5 human 54617 INO80 INO80 complex ATPase subunit XR_001751322.2 52.7% (many diffs)
6 mouse 68142 Ino80 INO80 complex subunit NM_026574.3 90.6% 96.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4737
ORF length:
4668
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcctcggag ttgggtgcca gggatgatgg aggctgcact gagctggcaa
121agcccctcta tcttcagtac ttggagaggg ccctccggtt ggaccatttt ctgcgacaaa
181cgtcagctat cttcaatagg aatatttcta gtgatgacag tgaagatgga ctggatgaca
241gtaatccatt attgccccag tctggggatc ccttaataca agttaaggaa gaacctccaa
301attcattgct tggtgaaact tctggagcag gcagttctgg aatgttaaac acatattctc
361tgaatggagt tctacagtca gaatcaaaat gtgataaggg gaatttatat aatttctcta
421agctgaagaa aagcagaaag tggctaaaga gcattctgct aagtgatgaa tccagcgagg
481ctgattctca gagtgaagac gatgatgaag aagaactcaa tctcagcaga gaagaacttc
541acaacatgct tcgactacac aaatataaga aacttcacca aaataagtat agtaaagaca
601aggagttgca gcaatatcag tactacagtg caggcctgct ctccacatat gaccctttct
661atgagcaaca acggcaccta cttggaccca agaaaaagaa atttaaggag gaaaagaaac
721ttaaagctaa gttgaaaaaa gtgaagaaaa aaagacgaag agatgaagaa ctttcctctg
781aagaatcccc tcgtcgccat caccaccaga ccaaagtctt tgccaagttt tctcacgatg
841cacctccccc tggcactaag aaaaagcact tatccattga gcagctgaat gctcgtcgca
901ggaaagtatg gctcagcatt gtgaaaaagg aactaccaaa ggcaaataag cagaaagctt
961cagctcgtaa cctgtttctc accaatagcc gaaagcttgc tcaccagtgc atgaaggagg
1021tgcgtcgagc tgccttgcag gcccagaaga actgtaagga aaccttgcct cgtgcccgcc
1081gcctcaccaa ggagatgctt ctgtactgga agaaatatga gaaagtagag aaggagcacc
1141gcaagagagc agagaaggaa gctttggagc agcggaagtt ggatgaggaa atgcgggagg
1201ccaagaggca acagcgaaaa ctcaacttct taattaccca gacagagttg tatgcccatt
1261tcatgagtcg caaacgagat atgggtcatg atggtatcca ggaagaaatc ctaaggaaac
1321tggaagacag ttctacccag agacaaatcg atataggtgg aggagtggta gttaacatca
1381cacaggagga ttatgatagt aaccatttta aagcccaggc cctgaagaat gctgaaaatg
1441cttaccatat tcaccaagct cggacaaggt catttgatga agatgcaaaa gaaagtcgag
1501cagctgccct acgggcagca aacaagtctg gcactgggtt tggggagagt tatagcctgg
1561ctaacccatc tatccgggct ggtgaggata ttccacagcc cacaattttt aatggcaaat
1621tgaaaggtta tcaactgaaa ggcatgaatt ggttggccaa tctatatgaa cagggtatta
1681atggcattct tgctgatgaa atgggccttg gtaaaacagt acagagcatt gcccttctgg
1741cccatctggc tgagagagag aacatttggg gacctttctt aataatttca cctgcgtcta
1801cacttaacaa ttggcaccag gagtttacta gatttgttcc taaatttaag gtgctaccat
1861attggggaaa tcctcatgat agaaaagtca tcagaaggtt ctggagtcag aagaccctct
1921acactcagga tgcccccttc catgtggtta ttaccagcta tcagctggtg gtgcaggatg
1981taaagtattt ccagcgggtc aagtggcaat acatggtact ggatgaggct caggcgctca
2041agagtagttc cagtgttcgt tggaagatcc tcttacagtt ccagtgtcgg aatcggcttt
2101tgctaaccgg gaccccaatt cagaacacca tggcagagct ttgggctctg ctgcatttca
2161ttatgccaac attatttgat tcacatgagg aatttaatga atggttttcc aaggacattg
2221agagccatgc cgaaaacaaa tctgctattg atgagaatca actttctcgc ttacacatga
2281ttttgaagcc atttatgctg aggagaatca agaaagatgt ggaaaatgaa ttatctgaca
2341agattgagat tctaatgtat tgccaactga ccagccgaca gaagctgcta tatcaggcac
2401taaagaacaa aatttccatt gaggatttat tgcagtcttc tatgggctct acccaacaag
2461cacagaacac caccagcagc ctcatgaatc tggtcatgca gtttaggaag gtgtgtaatc
2521acccggagtt atttgaacgg caagaaactt ggtctccatt tcatatttcc ctaaagccat
2581accacatttc aaagtttatc taccgtcatg gacagatcag ggtcttcaat cattcacgag
2641acaggtggtt aagggttctt tctccatttg caccagacta tatccaacgg tctctctttc
2701acagaaaagg tattaatgaa gaaagctgtt tctctttcct tcgctttatt gatatatctc
2761cagcagaaat ggcaaacctt atgcttcagg gacttttggc cagatggtta gctcttttcc
2821tgtctctgaa agcctcctac aggctccatc agctacgctc ctggggagcg ccagaagggg
2881agagccacca gagatacctg aggaacaagg atttccttct tggggttaat tttccactct
2941cctttccaaa cctttgcagc tgccctttgt taaagtctct tgttttcagc agccactgta
3001aagcagtgag tggctactca gaccaggttg tccatcagcg gagatcagct acctcctcgc
3061tgcgtcgctg cctgctcact gagctgccat cttttttgtg tgtggccagt ccacgagtta
3121ccgcagtgcc attggattct tactgcaatg accgaagtgc agaatatgaa aggcgagttc
3181tgaaggaagg agggagtctg gcagccaagc agtgtttgtt gaatggggcc cctgaactgg
3241ctgcagactg gctaaataga cgatcacagt tcttcccaga gccagctgga ggtctgtgga
3301gcatcagacc tcagaatggc tggtctttca tcaggattcc aggcaaggag agcctcatca
3361ctgacagtgg aaagctgtat gcccttgatg tcctgctgac tcggctcaag tctcaagggc
3421atagggtcct tatctactcc cagatgacca ggatgataga cctactggag gaatacatgg
3481tttacaggaa gcatacctac atgaggcttg atggctcatc caagatctcg gagaggcgag
3541acatggttgc tgattttcag aacaggaatg acatctttgt gttcctgtta agcacacgag
3601ctggaggact gggtatcaat ctcactgctg cagacacagt gattttctat gatagcgact
3661ggaaccccac tgtggaccag caggccatgg acagggccca ccgcttaggg cagacaaagc
3721aggttactgt gtaccggctc atctgtaaag gcaccattga agaacgcatt ctgcaaagag
3781ccaaggagaa gagtgagatt cagcggatgg tgatttcagg tgggaacttc aaaccagata
3841ccttgaaacc caaagaggtg gttagtcttc ttctagacga cgaagagttg gagaagaaac
3901tgaggctgcg gcaggaagag aaacggcaac aggaggaaac caaccgagtg aaagagcgca
3961agcggaagcg ggaaaagtat gcagagaaga agaaaaaaga agatgaattg gatgggaaaa
4021ggagaaaaga gggtgtgaac ctggtgatcc catttgttcc ctcggctgat aactccaacc
4081tctctgctga cggagatgac tccttcatta gcgtggactc agccatgcca agccctttca
4141gtgagatctc catcagcagt gagctgcaca ctggctccat tcccctggac gagagcagca
4201gtgacatgct ggtcattgtg gatgacccag cctcctcagc ccctcagtct cgagctacca
4261actctcccgc atccataaca ggctccgtct cagataccgt gaatggaatt tccattcagg
4321aaatgccagc tgcaggacgt ggtcactcag cccgaagccg aggccgcccc aaaggttcag
4381gaagcacagc caaaggagca gggaagggcc ggagccgaaa gtccacggca ggcagtgctg
4441ctgcaatggc aggagccaaa gccggggctg cagcggcctc tgcagctgcc tatgccgcat
4501acgggtacaa cgtgtctaaa ggaatctccg ccagcagtcc tctgcagaca tcccttgttc
4561ggcctgctgg ccttgctgac tttggacctt caagcgcctc ttctcctttg agttcccctt
4621tgagcaaggg aaataatgtt cctgggaatc ccaagaacct ccacatgacc agcagcctag
4681ccccagactc tctggtccgg aaacagggca aaggcaccaa cccctctgga ggacggttgc
4741caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
4801ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
4861tta

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