Construct: ORF ccsbBroad304_03442
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003670.1_s304c1, BRDN0000462361
- Derived from:
- ccsbBroadEn_03442
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INO80 (54617)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03442
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54617 | INO80 | INO80 complex ATPase subunit | NM_017553.3 | 100% | 100% | |
| 2 | human | 54617 | INO80 | INO80 complex ATPase subunit | XM_011521685.3 | 76.8% | 75.5% | (many diffs) |
| 3 | human | 54617 | INO80 | INO80 complex ATPase subunit | NR_104038.1 | 71.4% | 1_317del;3589_3590ins128;4858_6225del | |
| 4 | human | 54617 | INO80 | INO80 complex ATPase subunit | XM_011521686.3 | 58.2% | 57.6% | 0_1ins1807;32_33ins143 |
| 5 | human | 54617 | INO80 | INO80 complex ATPase subunit | XR_001751322.2 | 52.7% | (many diffs) | |
| 6 | mouse | 68142 | Ino80 | INO80 complex subunit | NM_026574.3 | 90.6% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4737
- ORF length:
- 4668
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctcggag ttgggtgcca gggatgatgg aggctgcact gagctggcaa 121 agcccctcta tcttcagtac ttggagaggg ccctccggtt ggaccatttt ctgcgacaaa 181 cgtcagctat cttcaatagg aatatttcta gtgatgacag tgaagatgga ctggatgaca 241 gtaatccatt attgccccag tctggggatc ccttaataca agttaaggaa gaacctccaa 301 attcattgct tggtgaaact tctggagcag gcagttctgg aatgttaaac acatattctc 361 tgaatggagt tctacagtca gaatcaaaat gtgataaggg gaatttatat aatttctcta 421 agctgaagaa aagcagaaag tggctaaaga gcattctgct aagtgatgaa tccagcgagg 481 ctgattctca gagtgaagac gatgatgaag aagaactcaa tctcagcaga gaagaacttc 541 acaacatgct tcgactacac aaatataaga aacttcacca aaataagtat agtaaagaca 601 aggagttgca gcaatatcag tactacagtg caggcctgct ctccacatat gaccctttct 661 atgagcaaca acggcaccta cttggaccca agaaaaagaa atttaaggag gaaaagaaac 721 ttaaagctaa gttgaaaaaa gtgaagaaaa aaagacgaag agatgaagaa ctttcctctg 781 aagaatcccc tcgtcgccat caccaccaga ccaaagtctt tgccaagttt tctcacgatg 841 cacctccccc tggcactaag aaaaagcact tatccattga gcagctgaat gctcgtcgca 901 ggaaagtatg gctcagcatt gtgaaaaagg aactaccaaa ggcaaataag cagaaagctt 961 cagctcgtaa cctgtttctc accaatagcc gaaagcttgc tcaccagtgc atgaaggagg 1021 tgcgtcgagc tgccttgcag gcccagaaga actgtaagga aaccttgcct cgtgcccgcc 1081 gcctcaccaa ggagatgctt ctgtactgga agaaatatga gaaagtagag aaggagcacc 1141 gcaagagagc agagaaggaa gctttggagc agcggaagtt ggatgaggaa atgcgggagg 1201 ccaagaggca acagcgaaaa ctcaacttct taattaccca gacagagttg tatgcccatt 1261 tcatgagtcg caaacgagat atgggtcatg atggtatcca ggaagaaatc ctaaggaaac 1321 tggaagacag ttctacccag agacaaatcg atataggtgg aggagtggta gttaacatca 1381 cacaggagga ttatgatagt aaccatttta aagcccaggc cctgaagaat gctgaaaatg 1441 cttaccatat tcaccaagct cggacaaggt catttgatga agatgcaaaa gaaagtcgag 1501 cagctgccct acgggcagca aacaagtctg gcactgggtt tggggagagt tatagcctgg 1561 ctaacccatc tatccgggct ggtgaggata ttccacagcc cacaattttt aatggcaaat 1621 tgaaaggtta tcaactgaaa ggcatgaatt ggttggccaa tctatatgaa cagggtatta 1681 atggcattct tgctgatgaa atgggccttg gtaaaacagt acagagcatt gcccttctgg 1741 cccatctggc tgagagagag aacatttggg gacctttctt aataatttca cctgcgtcta 1801 cacttaacaa ttggcaccag gagtttacta gatttgttcc taaatttaag gtgctaccat 1861 attggggaaa tcctcatgat agaaaagtca tcagaaggtt ctggagtcag aagaccctct 1921 acactcagga tgcccccttc catgtggtta ttaccagcta tcagctggtg gtgcaggatg 1981 taaagtattt ccagcgggtc aagtggcaat acatggtact ggatgaggct caggcgctca 2041 agagtagttc cagtgttcgt tggaagatcc tcttacagtt ccagtgtcgg aatcggcttt 2101 tgctaaccgg gaccccaatt cagaacacca tggcagagct ttgggctctg ctgcatttca 2161 ttatgccaac attatttgat tcacatgagg aatttaatga atggttttcc aaggacattg 2221 agagccatgc cgaaaacaaa tctgctattg atgagaatca actttctcgc ttacacatga 2281 ttttgaagcc atttatgctg aggagaatca agaaagatgt ggaaaatgaa ttatctgaca 2341 agattgagat tctaatgtat tgccaactga ccagccgaca gaagctgcta tatcaggcac 2401 taaagaacaa aatttccatt gaggatttat tgcagtcttc tatgggctct acccaacaag 2461 cacagaacac caccagcagc ctcatgaatc tggtcatgca gtttaggaag gtgtgtaatc 2521 acccggagtt atttgaacgg caagaaactt ggtctccatt tcatatttcc ctaaagccat 2581 accacatttc aaagtttatc taccgtcatg gacagatcag ggtcttcaat cattcacgag 2641 acaggtggtt aagggttctt tctccatttg caccagacta tatccaacgg tctctctttc 2701 acagaaaagg tattaatgaa gaaagctgtt tctctttcct tcgctttatt gatatatctc 2761 cagcagaaat ggcaaacctt atgcttcagg gacttttggc cagatggtta gctcttttcc 2821 tgtctctgaa agcctcctac aggctccatc agctacgctc ctggggagcg ccagaagggg 2881 agagccacca gagatacctg aggaacaagg atttccttct tggggttaat tttccactct 2941 cctttccaaa cctttgcagc tgccctttgt taaagtctct tgttttcagc agccactgta 3001 aagcagtgag tggctactca gaccaggttg tccatcagcg gagatcagct acctcctcgc 3061 tgcgtcgctg cctgctcact gagctgccat cttttttgtg tgtggccagt ccacgagtta 3121 ccgcagtgcc attggattct tactgcaatg accgaagtgc agaatatgaa aggcgagttc 3181 tgaaggaagg agggagtctg gcagccaagc agtgtttgtt gaatggggcc cctgaactgg 3241 ctgcagactg gctaaataga cgatcacagt tcttcccaga gccagctgga ggtctgtgga 3301 gcatcagacc tcagaatggc tggtctttca tcaggattcc aggcaaggag agcctcatca 3361 ctgacagtgg aaagctgtat gcccttgatg tcctgctgac tcggctcaag tctcaagggc 3421 atagggtcct tatctactcc cagatgacca ggatgataga cctactggag gaatacatgg 3481 tttacaggaa gcatacctac atgaggcttg atggctcatc caagatctcg gagaggcgag 3541 acatggttgc tgattttcag aacaggaatg acatctttgt gttcctgtta agcacacgag 3601 ctggaggact gggtatcaat ctcactgctg cagacacagt gattttctat gatagcgact 3661 ggaaccccac tgtggaccag caggccatgg acagggccca ccgcttaggg cagacaaagc 3721 aggttactgt gtaccggctc atctgtaaag gcaccattga agaacgcatt ctgcaaagag 3781 ccaaggagaa gagtgagatt cagcggatgg tgatttcagg tgggaacttc aaaccagata 3841 ccttgaaacc caaagaggtg gttagtcttc ttctagacga cgaagagttg gagaagaaac 3901 tgaggctgcg gcaggaagag aaacggcaac aggaggaaac caaccgagtg aaagagcgca 3961 agcggaagcg ggaaaagtat gcagagaaga agaaaaaaga agatgaattg gatgggaaaa 4021 ggagaaaaga gggtgtgaac ctggtgatcc catttgttcc ctcggctgat aactccaacc 4081 tctctgctga cggagatgac tccttcatta gcgtggactc agccatgcca agccctttca 4141 gtgagatctc catcagcagt gagctgcaca ctggctccat tcccctggac gagagcagca 4201 gtgacatgct ggtcattgtg gatgacccag cctcctcagc ccctcagtct cgagctacca 4261 actctcccgc atccataaca ggctccgtct cagataccgt gaatggaatt tccattcagg 4321 aaatgccagc tgcaggacgt ggtcactcag cccgaagccg aggccgcccc aaaggttcag 4381 gaagcacagc caaaggagca gggaagggcc ggagccgaaa gtccacggca ggcagtgctg 4441 ctgcaatggc aggagccaaa gccggggctg cagcggcctc tgcagctgcc tatgccgcat 4501 acgggtacaa cgtgtctaaa ggaatctccg ccagcagtcc tctgcagaca tcccttgttc 4561 ggcctgctgg ccttgctgac tttggacctt caagcgcctc ttctcctttg agttcccctt 4621 tgagcaaggg aaataatgtt cctgggaatc ccaagaacct ccacatgacc agcagcctag 4681 ccccagactc tctggtccgg aaacagggca aaggcaccaa cccctctgga ggacggttgc 4741 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 4801 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 4861 tta