Construct: ORF ccsbBroad304_03591

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002858.1_s304c1, BRDN0000407713
Derived from:
ccsbBroadEn_03591
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ETNK1 (55500)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03591
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55500 ETNK1 ethanolamine kinase 1 XM_017019580.1 91.9% 89.4% (many diffs)
2 human 55500 ETNK1 ethanolamine kinase 1 NM_018638.5 80.3% 80.3% 0_1ins267
3 human 55500 ETNK1 ethanolamine kinase 1 XM_017019581.1 72.2% 71% (many diffs)
4 human 55500 ETNK1 ethanolamine kinase 1 XM_005253420.4 56.6% 53.3% (many diffs)
5 human 55500 ETNK1 ethanolamine kinase 1 NM_001039481.2 35.1% 32.7% (many diffs)
6 human 55500 ETNK1 ethanolamine kinase 1 XR_002957349.1 18.8% 1_19del;986_1088del;1479_7192del
7 mouse 75320 Etnk1 ethanolamine kinase 1 NM_029250.2 70.7% 75.4% (many diffs)
8 mouse 75320 Etnk1 ethanolamine kinase 1 XM_006507011.3 52.7% 55.5% (many diffs)
9 mouse 75320 Etnk1 ethanolamine kinase 1 XM_006507012.1 45.4% 48.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1422
ORF length:
1356
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct ctgcggccgc ccgcggtcca gctccgacaa caggaatttt ctccgagagc
121gggccgggct cagttcagct gctgtccaga cccggatcgg caacagtgcc gcctccagac
181gttctcctgc cgctcgcccg cccgtcccag cgcccccagc cctcccgcga gggcgccccg
241ggacggaagg atccaccagt ctgtcggcgc ccgccgttct cgtggtcgcc gtcgccgtcg
301tcgtggtggt agtctccgcc gtcgcctggg ccatggccaa ttacatccac gtccctcccg
361gctccccgga ggtgcccaag ctgaacgtca ccgttcagga tcaggaggag catcgctgcc
421gggagggggc cctgagcctc ctgcaacacc tgcggcctca ctgggacccc caggaggtga
481ccctgcagct cttcacagat ggaatcacaa ataaacttat tggctgttac gtgggaaaca
541ccatggagga tgtagtcctg gtgagaattt atggcaataa gactgagtta ttagtcgatc
601gagatgagga agtaaagagt tttcgagtgt tgcaggctca tgggtgtgca ccacaactct
661actgtacctt caataatgga ctatgctatg aatttataca aggagaagca ctggatccaa
721agcatgtctg caacccagcc attttcaggc taatagctcg tcagcttgct aaaatccatg
781ctattcatgc acacaatggc tggatcccca aatctaatct ttggctaaag atgggaaagt
841atttctctct cattcccaca ggatttgcag atgaagacat taataaaagg ttcctaagtg
901atatcccaag ctctcagatt ctccaggaag agatgacttg gatgaaggag attctttcca
961acctgggctc acctgttgtg ctttgccata atgacctatt gtgtaagaat ataatctaca
1021atgagaaaca aggtgatgta cagttcattg attatgaata ttctggatac aactacctgg
1081catatgatat tggaaatcat ttcaatgaat ttgcaggtgt gagtgatgta gactatagtc
1141tgtatccaga tagagaacta cagagtcagt ggctgcgtgc ttaccttgaa gcctacaaag
1201aatttaaggg ctttgggact gaagttactg aaaaggaggt agaaatactc ttcattcaag
1261tcaatcagtt tgcattggct tctcatttct tttggggatt gtgggctttg attcaagcca
1321aatactccac tattgagttt gatttccttg ggtatgcaat tgttcgtttt aaccagtact
1381ttaaaatgaa gcctgaggtt actgcattaa aagtgcctga gtacccaact ttcttgtaca
1441aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1501ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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