Construct: ORF ccsbBroad304_04089
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016566.1_s304c1, BRDN0000409002
- Derived from:
- ccsbBroadEn_04089
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ACSS3 (79611)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04089
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79611 | ACSS3 | acyl-CoA synthetase short c... | NM_024560.4 | 100% | 100% | |
| 2 | human | 79611 | ACSS3 | acyl-CoA synthetase short c... | NM_001330242.2 | 99.8% | 99.8% | 307_308insGGT |
| 3 | human | 79611 | ACSS3 | acyl-CoA synthetase short c... | XR_944720.3 | 79.9% | 1_84del;1803_1928del;2024_2025ins244 | |
| 4 | human | 79611 | ACSS3 | acyl-CoA synthetase short c... | XM_005269151.5 | 65.7% | 65.7% | 1355_1360delGTATAA;1363delC;1365_1366ins700 |
| 5 | human | 79611 | ACSS3 | acyl-CoA synthetase short c... | NM_001330243.2 | 53.6% | 53.6% | 0_1ins954 |
| 6 | mouse | 380660 | Acss3 | acyl-CoA synthetase short-c... | NM_001142804.1 | 85.7% | 90.1% | (many diffs) |
| 7 | mouse | 380660 | Acss3 | acyl-CoA synthetase short-c... | NM_198636.3 | 60.5% | 62.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2124
- ORF length:
- 2058
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttgagatgaa accgtcttgg ctgcagtgtc gtaaagtcac cagcgccggg gggctcggag 121 ggcccttgcc tgggtcctct ccggcccggg gagccggtgc ggccctcagg gctttagtgg 181 tcccgggccc gcggggcggt ctcgggggcc ggggatgcag ggcactgtcc tccggcagtg 241 gcagcgagta caagacccac ttcgcagcct cggtgaccga ccccgagagg ttctggggca 301 aagctgccga gcagatcagc tggtacaagc cctggaccaa aacgctggag aacaaacact 361 cgccctctac caggtggttt gtggaaggaa tgcttaacat ttgttacaat gccgttgatc 421 gtcatattga aaatggtaaa ggggataaga ttgctatcat ctatgacagt cctgttacaa 481 acactaaagc aacctttacc tataaagaag ttctggagca ggtctccaag ctggctggtg 541 tcttggtcaa gcatggcatc aagaaaggtg acactgtggt tatctacatg cctatgatcc 601 cacaggcgat gtataccatg ttggcatgtg caaggatagg tgccatccac agtctcatat 661 ttggaggatt tgcttccaaa gaactaagta gtcgcattga tcatgtaaag cccaaggtgg 721 ttgttacagc atcatttggc attgaacctg gaaggagggt agagtacgta ccacttgtag 781 aagaagcgct aaaaatagga caacacaaac cagacaaaat tctcatttat aatcgtccaa 841 atatggaggc ggttcctttg gctcccggtc gtgaccttga ttgggatgaa gagatggcaa 901 aagcccagtc acatgactgt gttcctgttc tttcagaaca cccactgtat attctttaca 961 catctggcac aacggggtta cctaagggtg tgattaggcc cactggggga tacgctgtca 1021 tgctacactg gtcaatgtct tccatatacg gacttcaacc cggagaggtg tggtgggcag 1081 cttctgactt aggctgggtt gttggacatt cctatatctg ctatggacct cttcttcatg 1141 ggaacacaac agttttatat gaggggaagc ctgtgggaac accagatgct ggcgcttatt 1201 tccgtgtgct tgcagagcat ggagtagctg ccttgtttac agcaccaact gcaattagag 1261 caatccgtca acaggaccct ggggcagctt tggggaagca gtactctctg acaaggttca 1321 aaacattatt tgtggctgga gaacgatgtg atgtagagac cctggaatgg tccaaaaatg 1381 tcttcagagt acctgtctta gaccattggt ggcaaactga gactggatct ccaattactg 1441 cgtcatgtgt tggattaggc aattctaaaa cacctccacc agggcaagca ggaaaaagcg 1501 tcccaggata caatgttatg attttggatg acaacatgca aaaactgaag gctcggtgtt 1561 taggaaatat tgtggtaaag ttaccattgc cacctggggc tttttcagga ctctggaaga 1621 atcaggaagc attcaagcat ttatactttg aaaaatttcc tggatattat gataccatgg 1681 atgctggtta catggatgaa gaaggctatt tgtatgttat gtctcgagtg gatgatgtaa 1741 taaatgttgc aggtcacaga atttctgcag gcgccattga agagtcaatc ctttcccatg 1801 gtaccgtggc agactgtgct gttgttggca aggaagatcc cttaaaaggt catgtcccct 1861 tagcactctg tgtattgaga aaagatataa atgcaacaga ggagcaagtt ttggaagaaa 1921 ttgtgaaaca cgttagacag aacattggcc ctgtggctgc ttttcgaaat gcagtgtttg 1981 tcaaacagct acccaaaacc agatctggca agatcccccg atcagcttta tctgccattg 2041 tcaatggcaa gccatacaag ataacttcta caattgaaga ccccagcatt tttggccacg 2101 tagaagaaat gctgaagcaa gcataatcaa ctttcttgta caaagtggtt ggtaagccta 2161 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 2221 gcgttaagtc gacaatcaac ctctggatta