Transcript: Human NM_024560.4

Homo sapiens acyl-CoA synthetase short chain family member 3 (ACSS3), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-13
Taxon:
Homo sapiens (human)
Gene:
ACSS3 (79611)
Length:
8391
CDS:
43..2103

Additional Resources:

NCBI RefSeq record:
NM_024560.4
NBCI Gene record:
ACSS3 (79611)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_024560.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000151676 GCTCGGTGTTTAGGAAATATT pLKO.1 1528 CDS 100% 15.000 21.000 N ACSS3 n/a
2 TRCN0000343483 GCTCGGTGTTTAGGAAATATT pLKO_005 1528 CDS 100% 15.000 21.000 N ACSS3 n/a
3 TRCN0000152949 GCCATTGAAGAGTCAATCCTT pLKO.1 1750 CDS 100% 0.300 0.240 N ACSS3 n/a
4 TRCN0000153547 CCTCCCAAGAAGAACACTTTA pLKO.1 2389 3UTR 100% 13.200 9.240 N ACSS3 n/a
5 TRCN0000343484 CCTCCCAAGAAGAACACTTTA pLKO_005 2389 3UTR 100% 13.200 9.240 N ACSS3 n/a
6 TRCN0000153830 CCACTTCCTGAAACCAAGAAT pLKO.1 2434 3UTR 100% 5.625 3.938 N ACSS3 n/a
7 TRCN0000343485 CCACTTCCTGAAACCAAGAAT pLKO_005 2434 3UTR 100% 5.625 3.938 N ACSS3 n/a
8 TRCN0000152146 CGAAATGCAGTGTTTGTCAAA pLKO.1 1942 CDS 100% 4.950 3.465 N ACSS3 n/a
9 TRCN0000154774 GCTGGAGAACGATGTGATGTA pLKO.1 1312 CDS 100% 4.950 3.465 N ACSS3 n/a
10 TRCN0000343482 GCTGGAGAACGATGTGATGTA pLKO_005 1312 CDS 100% 4.950 3.465 N ACSS3 n/a
11 TRCN0000155379 CATGTGAAAGACCTGTGCCTT pLKO.1 2256 3UTR 100% 2.640 1.848 N ACSS3 n/a
12 TRCN0000153386 GTTTGTCAAACAGCTACCCAA pLKO.1 1953 CDS 100% 2.640 1.848 N ACSS3 n/a
13 TRCN0000150379 CCATTGAAGAGTCAATCCTTT pLKO.1 1751 CDS 100% 0.495 0.347 N ACSS3 n/a
14 TRCN0000431521 TTGTTGGACATTCCTATATTT pLKO_005 1076 CDS 100% 15.000 10.500 N Acss3 n/a
15 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 2813 3UTR 100% 4.950 2.475 Y LOC339059 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_024560.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04089 pDONR223 99.3% 100% 100% None n/a
2 ccsbBroad304_04089 pLX_304 0% 100% 100% V5 (not translated due to prior stop codon) n/a
3 TRCN0000474580 AACCATATGTGTTGTCGACATTGC pLX_317 15.6% 100% 100% V5 (not translated due to prior stop codon) n/a
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