Construct: ORF ccsbBroad304_04150

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017603.1_s304c1, BRDN0000409263
Derived from:
ccsbBroadEn_04150
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NOL10 (79954)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04150
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79954 NOL10 nucleolar protein 10 NM_024894.4 100% 100%
2 human 79954 NOL10 nucleolar protein 10 NM_001261392.1 96.2% 96.2% 210_211ins78
3 human 79954 NOL10 nucleolar protein 10 NM_001261394.1 92.7% 92.7% 756_757ins150
4 human 79954 NOL10 nucleolar protein 10 XM_011510398.3 78% 77.6% (many diffs)
5 human 79954 NOL10 nucleolar protein 10 XM_011510399.2 78% 77.6% (many diffs)
6 human 79954 NOL10 nucleolar protein 10 XR_002959342.1 76.4% 1_114del;1020_1021ins67;2112_2546del
7 human 79954 NOL10 nucleolar protein 10 XR_001738957.1 75.5% 1_114del;1141_1275del;2314_2732del
8 human 79954 NOL10 nucleolar protein 10 NR_048552.1 57.4% 1_126del;190_191ins46;2145_3469del
9 human 79954 NOL10 nucleolar protein 10 XM_011510400.2 51.3% 50.7% (many diffs)
10 human 79954 NOL10 nucleolar protein 10 XM_011510402.2 50% 49.8% (many diffs)
11 human 79954 NOL10 nucleolar protein 10 XM_024453152.1 44.2% 43.8% (many diffs)
12 human 79954 NOL10 nucleolar protein 10 XM_024453153.1 44.2% 43.8% (many diffs)
13 mouse 217431 Nol10 nucleolar protein 10 NM_001008421.1 88.6% 95.4% (many diffs)
14 mouse 217431 Nol10 nucleolar protein 10 XM_017315015.1 81.9% 88.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2130
ORF length:
2064
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca ggtctccagc ctcaatgagg tgaagattta cagcctcagc tgcggcaagt
121cccttcctga gtggctttct gataggaaga agagagcgct acagaagaaa gatgtagatg
181tccgtaggag aattgaactt attcaggact ttgaaatgcc tactgtgtgt accactatta
241aggtgtcaaa agatggacag tacattttag caactggaac atataaacct cgggttcgat
301gttatgacac ctatcaatta tccttgaagt ttgaaaggtg tttagattca gaagttgtca
361cctttgaaat tttgtctgat gactactcaa agattgtctt cttacataat gatagataca
421ttgaatttca ttcgcaatca ggtttttact acaaaaccag aataccaaag tttgggagag
481atttctctta ccactatcca tcctgtgact tgtactttgt tggtgcaagt tctgaagttt
541ataggttaaa cttagaacaa ggacgatacc tgaatcctct acaaactgat gctgcggaga
601ataatgtttg tgacataaat tcagtgcatg gcttgtttgc cacaggaacc atagagggta
661gagtggagtg ctgggaccca agaactcgaa acagagttgg cctgttagac tgcgccttaa
721acagtgtcac agcagattca gagataaaca gtttaccaac aatctctgct ttgaaattta
781atggtgcctt gaccatggca gttggaacaa ccacagggca ggttttatta tatgaccttc
841gatctgataa gccattgcta gttaaagatc accagtatgg gctgcccatt aagtccgttc
901atttccagga ttcattagat ctgattttgt ctgctgactc tcgaattgtc aagatgtgga
961ataagaactc cggaaaaata tttacttcct tggagccaga gcatgacctt aatgatgttt
1021gtctctaccc caactcaggc atgcttctga cggccaatga aacccccaag atgggcatct
1081attacattcc agttttgggt cctgctcctc ggtggtgttc cttcttagac aacttgaccg
1141aagaattaga agagaatcca gaaagcacag tctatgatga ttataaattt gtcaccaaga
1201aagaccttga aaatttaggg ctcacccacc tcattggatc tcctttcctc cgggcatata
1261tgcatgggtt tttcatggat ataagactct atcacaaggt gaaactgatg gtaaatccat
1321ttgcttatga agaatatagg aaagataaaa tacgacagaa aatagaagaa acacgtgcac
1381agagagtcca gttaaagaaa ttgccaaaag ttaacaaaga gctggcactt aaattaattg
1441aggaagaaga ggagaagcag aaatctacat ggaaaaagaa agttaagagt cttcctaata
1501ttctcaccga tgatcgattt aaagttatgt ttgagaaccc tgacttccaa gtagatgaag
1561agagtgaaga atttaggctt ctgaatccac ttgtttcaaa aattagtgaa aaaaggaaga
1621agaaactaag actcttagag caacaagaac ttcgtgaaaa agaagaggag gaagagccgg
1681aaggaaaacc aagtgatgca gaaagttcgg agagttcaga tgatgaaaaa gcctgggttg
1741aagaggtcag gaagcaacgc agactcctcc agcaggagga aaaagtgaag cggcaggaac
1801gactcaagga ggaccagcag acagtcctaa agccccagtt ttatgagatc aaagcaggag
1861aagaatttag aagcttcaaa gattctgcca caaagcagaa actgatgaac aaaacccttg
1921aagatcgttt gaaaattgaa gcaaaaaatg ggacattgag tgtatccgac accaccgttg
1981gcagcaaaca attgacattc acgttaaaga ggtctgaaca gcagaagaag caacaggagg
2041ctgagaaact gcatcgacaa gaaaggaaaa gactccgtcg ttcggccgga cacctgaagt
2101caagacacaa aagaggacgg tcgtttcatt gcccaacttt cttgtacaaa gtggttggta
2161agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
2221ggtggcgcgt taagtcgaca atcaacctct ggatta

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