Transcript: Mouse XM_017315015.1

PREDICTED: Mus musculus nucleolar protein 10 (Nol10), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Nol10 (217431)
Length:
2668
CDS:
280..2193

Additional Resources:

NCBI RefSeq record:
XM_017315015.1
NBCI Gene record:
Nol10 (217431)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017315015.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000215696 CGTGTTAGACTGTGCATTAAA pLKO.1 765 CDS 100% 15.000 21.000 N Nol10 n/a
2 TRCN0000176344 CGAGCATATATGCATGGGTTT pLKO.1 1315 CDS 100% 4.050 5.670 N Nol10 n/a
3 TRCN0000193946 GTCCGGTGTTATGACACATAT pLKO.1 358 CDS 100% 13.200 10.560 N Nol10 n/a
4 TRCN0000174614 GAGCACAGTGTATGATGATTA pLKO.1 1227 CDS 100% 13.200 9.240 N Nol10 n/a
5 TRCN0000174359 CAAGCTCTGAAGTTTATAGAT pLKO.1 590 CDS 100% 5.625 3.938 N Nol10 n/a
6 TRCN0000174712 GCATCATTTCAGATATTGGAA pLKO.1 2429 3UTR 100% 3.000 2.100 N Nol10 n/a
7 TRCN0000174869 GCAGTTGACATTTACTCTCAA pLKO.1 2049 CDS 100% 4.950 2.970 N Nol10 n/a
8 TRCN0000193300 CCAATTAAAGAAACTGCCAAA pLKO.1 1452 CDS 100% 4.050 2.430 N Nol10 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017315015.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04150 pDONR223 100% 81.9% 88.3% None (many diffs) n/a
2 ccsbBroad304_04150 pLX_304 0% 81.9% 88.3% V5 (many diffs) n/a
3 TRCN0000474127 CCTCGCTTTTACACCCGGAGTCTA pLX_317 20.9% 81.9% 88.3% V5 (many diffs) n/a
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