Construct: ORF ccsbBroad304_04157
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007958.1_s304c1, BRDN0000408822
- Derived from:
- ccsbBroadEn_04157
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRHL2 (79977)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04157
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79977 | GRHL2 | grainyhead like transcripti... | NM_024915.4 | 100% | 100% | |
| 2 | human | 79977 | GRHL2 | grainyhead like transcripti... | NM_001330593.2 | 97.4% | 97.4% | 0_1ins48 |
| 3 | human | 79977 | GRHL2 | grainyhead like transcripti... | XM_011517306.3 | 97.4% | 97.4% | 0_1ins48 |
| 4 | human | 79977 | GRHL2 | grainyhead like transcripti... | XM_011517307.3 | 94% | 94% | (many diffs) |
| 5 | mouse | 252973 | Grhl2 | grainyhead-like 2 (Drosophila) | NM_026496.4 | 88.3% | 94.7% | (many diffs) |
| 6 | mouse | 252973 | Grhl2 | grainyhead-like 2 (Drosophila) | XM_006520063.3 | 78.3% | 83.6% | (many diffs) |
| 7 | mouse | 252973 | Grhl2 | grainyhead-like 2 (Drosophila) | XM_017316621.1 | 78.3% | 83.6% | (many diffs) |
| 8 | mouse | 252973 | Grhl2 | grainyhead-like 2 (Drosophila) | XM_006520065.3 | 62.7% | 64.2% | (many diffs) |
| 9 | mouse | 252973 | Grhl2 | grainyhead-like 2 (Drosophila) | XM_017316622.1 | 55.6% | 56.4% | (many diffs) |
| 10 | mouse | 252973 | Grhl2 | grainyhead-like 2 (Drosophila) | XM_017316623.1 | 53.9% | 56.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1944
- ORF length:
- 1875
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcacaagag tcggacaata ataaaagact agtggcctta gtgcccatgc 121 ccagtgaccc tccattcaat acccgaagag cctacaccag tgaggatgaa gcctggaagt 181 catacttgga gaatcccctg acagcagcca ccaaggccat gatgagcatt aatggtgatg 241 aggacagtgc tgctgccctc ggcctgctct atgactacta caaggttcct cgagacaaga 301 ggctgctgtc tgtaagcaaa gcaagtgaca gccaagaaga ccaggagaaa agaaactgcc 361 ttggcaccag tgaagcccag agtaatttga gtggaggaga aaaccgagtg caagtcctaa 421 agactgttcc agtgaacctt tccctaaatc aagatcacct ggagaattcc aagcgggaac 481 agtacagcat cagcttcccc gagagctctg ccatcatccc ggtgtcggga atcacggtgg 541 tgaaagctga agatttcaca ccagttttca tggccccacc tgtgcactat ccccggggag 601 atggggaaga gcaacgagtg gttatctttg aacagactca gtatgacgtg ccctcgctgg 661 ccacccacag cgcctatctc aaagacgacc agcgcagcac tccggacagc acatacagcg 721 agagcttcaa ggacgcagcc acagagaaat ttcggagtgc ttcagttggg gctgaggagt 781 acatgtatga tcagacatca agtggcacat ttcagtacac cctggaagcc accaaatctc 841 tccgtcagaa gcagggggag ggccccatga cctacctcaa caaaggacag ttctatgcca 901 taacactcag cgagaccgga gacaacaaat gcttccgaca ccccatcagc aaagtcagga 961 gtgtggtgat ggtggtcttc agtgaagaca aaaacagaga tgaacagctc aaatactgga 1021 aatactggca ctctcggcag catacggcga agcagagggt ccttgacatt gccgattaca 1081 aggagagctt taatacgatt ggaaacattg aagagattgc atataatgct gtttccttta 1141 cctgggacgt gaatgaagag gcgaagattt tcatcaccgt gaattgcttg agcacagatt 1201 tctcctccca aaaaggggtg aaaggacttc ctttgatgat tcagattgac acatacagtt 1261 ataacaatcg tagcaataaa cccattcata gagcttattg ccagatcaag gtcttctgtg 1321 acaaaggagc agaaagaaaa atccgagatg aagagcggaa gcagaacagg aagaaaggga 1381 aaggccaggc ctcccaaact caatgcaaca gctcctctga tgggaagttg gctgccatac 1441 ctttacagaa gaagagtgac atcacctact tcaaaaccat gcctgatctc cactcacagc 1501 cagttctctt catacctgat gttcactttg caaacctgca gaggaccgga caggtgtatt 1561 acaacacgga tgatgaacga gaaggtggca gtgtccttgt taaacggatg ttccggccca 1621 tggaagagga gtttggtcca gtgccttcaa agcagatgaa agaagaaggg acaaagcgag 1681 tgctcttgta cgtgaggaag gagactgacg atgtgttcga tgcattgatg ttgaagtctc 1741 ccacagtgaa gggcctgatg gaagcgatat ctgagaaata tgggctgccc gtggagaaga 1801 tagcaaagct ttacaagaaa agcaaaaaag gcatcttggt gaacatggat gacaacatca 1861 tcgagcacta ctcgaacgag gacaccttca tcctcaacat ggagagcatg gtggagggct 1921 tcaaggtcac gctcatggaa atcttgccaa ctttcttgta caaagtggtt ggtaagccta 1981 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 2041 gcgttaagtc gacaatcaac ctctggatta