Construct: ORF ccsbBroad304_04460

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000044.1_s304c1, BRDN0000403890
Derived from:
ccsbBroadEn_04460
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FRMD5 (84978)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04460
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84978 FRMD5 FERM domain containing 5 NM_032892.5 100% 100%
2 human 84978 FRMD5 FERM domain containing 5 NM_001322950.2 89.1% 84.5% 1476_1477ins179;1524_1525insTGACACC
3 human 84978 FRMD5 FERM domain containing 5 XM_005254730.3 88.6% 84.6% (many diffs)
4 human 84978 FRMD5 FERM domain containing 5 NM_001322949.2 86.7% 84.1% (many diffs)
5 human 84978 FRMD5 FERM domain containing 5 NM_001322951.2 80.7% 78.1% (many diffs)
6 human 84978 FRMD5 FERM domain containing 5 NM_001286490.2 72.6% 68.4% (many diffs)
7 human 84978 FRMD5 FERM domain containing 5 NM_001286491.2 58.9% 56.9% 0_1ins613;25_26ins89
8 human 84978 FRMD5 FERM domain containing 5 XR_931927.3 36.4% 1_201del;1912_4685del
9 human 84978 FRMD5 FERM domain containing 5 NR_104455.2 31.8% (many diffs)
10 mouse 228564 Frmd5 FERM domain containing 5 XM_006499269.3 92.6% 98.4% (many diffs)
11 mouse 228564 Frmd5 FERM domain containing 5 XM_011239466.2 88.1% 93.6% (many diffs)
12 mouse 228564 Frmd5 FERM domain containing 5 XM_017317742.1 82.1% 83.1% (many diffs)
13 mouse 228564 Frmd5 FERM domain containing 5 NM_172673.3 81% 81.9% (many diffs)
14 mouse 228564 Frmd5 FERM domain containing 5 XM_017317740.1 80.2% 82.2% (many diffs)
15 mouse 228564 Frmd5 FERM domain containing 5 XM_017317739.1 78.1% 79.2% (many diffs)
16 mouse 228564 Frmd5 FERM domain containing 5 XM_017317738.1 76.4% 78.7% (many diffs)
17 mouse 228564 Frmd5 FERM domain containing 5 XM_017317734.1 76.3% 78.4% (many diffs)
18 mouse 228564 Frmd5 FERM domain containing 5 XM_017317736.1 75.9% 77.9% (many diffs)
19 mouse 228564 Frmd5 FERM domain containing 5 XM_011239469.2 74.1% 78.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1776
ORF length:
1710
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct gagcaggttg atgagcggca gcagcaggag cctggagcgc gagtacagct
121gcaccgtgcg gctgctggac gacagcgagt acacctgcac catccagaga gatgccaaag
181gccagtacct gtttgacctt ctttgccacc atctgaacct acttgagaaa gactattttg
241gtatccgctt tgtagaccca gataagcagc ggcattggct ggaatttaca aagtctgtgg
301tgaaacaatt gagatcccag cctccattca ccatgtgctt ccgtgtgaag ttttatcctg
361cagaccctgc tgctctgaaa gaagaaataa ccaggtattt agtcttcctg cagatcaaaa
421gggatctcta ccatggccga ctcctctgta aaacatcgga tgctgccttg ttagcagctt
481acatccttca agcggagatt ggggattatg actcagggaa acaccctgaa ggctacagct
541ccaagttcca gtttttccct aaacattcag agaagctgga aaggaaaatt gctgagattc
601acaagacgga actgagtggt caaacaccag caacatcaga gctgaacttc ttaagaaaag
661cacagacatt ggaaacatat ggagtggatc ctcacccatg taaggacgtg tcaggaaatg
721ctgcatttct ggccttcact ccttttgggt ttgttgttct tcaaggaaac aagagggtcc
781acttcattaa atggaatgag gtgaccaagc tgaaatttga aggaaagact ttctatttat
841acgtaagtca gaaagaggaa aagaaaatta ttcttacata ttttgctcca actcctgaag
901cgtgtaagca cctctggaaa tgtggaatcg agaaccaagc cttctacaag ctggagaagt
961caagccaagt ccgcacagtg tccagcagca atttattctt taaagggagc cggttccgat
1021acagtggccg agttgcaaag gaagtcatgg aatcaagtgc taagatcaaa cgggagccac
1081cggaaataca cagagcaggg atggttccca gccggagctg tccctccata acccatggcc
1141caaggctgag cagcgtcccc aggacccgca gaagagctgt tcacatctcc atcatggaag
1201gcctagagtc cttacgggac agtgcccatt ccacaccagt gcgttccact tcccatgggg
1261acaccttcct gcctcacgtg agaagcagcc ggacagatag caatgagcga gtagctgtga
1321ttgcagacga ggcctacagc cctgcagaca gcgtgctgcc cacccctgtg gctgagcaca
1381gcctggagct gatgttgctt tcccggcaga tcaatggagc cacctgcagc attgaggagg
1441agaaggaatc tgaagccagc accccaactg ctacagaggt ggaggccctt gggggagagc
1501tgagggccct gtgtcagggg cacagcgggc ccgaggagga acaggtgaat aagtttgttc
1561taagtgtcct ccgtttgctc cttgtgacca tgggactcct ctttgttttg ctcctcctcc
1621tgatcatcct taccgagtct gaccttgaca ttgccttttt ccgtgatatc cgccagaccc
1681ccgagtttga acaattccac tatcaatact tttgtcccct caggcgatgg tttgcctgca
1741aaatccgctc agtggtgagc ctgctcattg acacctgccc aactttcttg tacaaagtgg
1801ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1861ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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