Transcript: Mouse XM_011239469.2

PREDICTED: Mus musculus FERM domain containing 5 (Frmd5), transcript variant X8, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Frmd5 (228564)
Length:
4887
CDS:
229..1761

Additional Resources:

NCBI RefSeq record:
XM_011239469.2
NBCI Gene record:
Frmd5 (228564)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011239469.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000071901 CCGCCTTGTTAGCGGCCTATA pLKO.1 359 CDS 100% 3.600 2.880 N Frmd5 n/a
2 TRCN0000434856 TGAACAATTCCACTATCAATA pLKO_005 1671 CDS 100% 13.200 9.240 N FRMD5 n/a
3 TRCN0000071900 GCACCTCTGGAAATGTGGAAT pLKO.1 891 CDS 100% 4.950 3.465 N Frmd5 n/a
4 TRCN0000071899 GCTGAGATTCACAAGACCGAA pLKO.1 487 CDS 100% 2.640 1.848 N Frmd5 n/a
5 TRCN0000071898 CCCAGAATCTTCAGGTCATTT pLKO.1 4382 3UTR 100% 13.200 7.920 N Frmd5 n/a
6 TRCN0000420899 CAGTGTCCAGCAGCAATTTAT pLKO_005 959 CDS 100% 15.000 10.500 N FRMD5 n/a
7 TRCN0000427102 ACCTACTTGAGAAAGACTATT pLKO_005 113 5UTR 100% 13.200 9.240 N FRMD5 n/a
8 TRCN0000166364 CACACACACACACACACACAA pLKO.1 2995 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011239469.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04460 pDONR223 100% 74.1% 78.7% None (many diffs) n/a
2 ccsbBroad304_04460 pLX_304 0% 74.1% 78.7% V5 (many diffs) n/a
3 TRCN0000474059 TCGCGCAACGAACCCGAAAAGTAT pLX_317 21.4% 74.1% 78.7% V5 (many diffs) n/a
4 TRCN0000489266 GTTACTGTCGCAATGCCTAACCAT pLX_317 21% 74.1% 78.7% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000488208 TTAAATAGAAGTAGCAAACTCACT pLX_317 16.9% 74% 78.6% V5 (many diffs) n/a
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