Construct: ORF ccsbBroad304_04524
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008220.1_s304c1, BRDN0000406988
- Derived from:
- ccsbBroadEn_04524
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- N4BP2L1 (90634)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04524
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NM_001079691.2 | 100% | 100% | |
| 2 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NM_001286460.2 | 95.3% | 95.5% | 519_543delinsA |
| 3 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NM_001286461.2 | 87.5% | 66.8% | (many diffs) |
| 4 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NM_001353634.2 | 86.7% | 86.7% | 393_394ins69 |
| 5 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NM_001353630.2 | 80.9% | 76.9% | 464_569del;612_613insTGAAAGAACGGTA |
| 6 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NM_001353632.2 | 80.4% | 64.4% | (many diffs) |
| 7 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NM_001353635.2 | 80.3% | 61.3% | 306_307ins89;417_418insTGAAAGAACGGTA |
| 8 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | XM_017020841.2 | 72.3% | 66.8% | (many diffs) |
| 9 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NM_001353636.2 | 67% | 56.9% | 306_307ins158;348_349insTGAAAGAACGGTA |
| 10 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NM_001286459.2 | 65.8% | 53.4% | (many diffs) |
| 11 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NM_052818.3 | 65.8% | 53.4% | (many diffs) |
| 12 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NM_001353628.2 | 65.2% | 65.2% | 178_453del |
| 13 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NM_001353637.2 | 51.1% | 34% | (many diffs) |
| 14 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | XM_011535303.2 | 48.4% | 33.4% | (many diffs) |
| 15 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NM_001353627.2 | 48% | 39.4% | (many diffs) |
| 16 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NM_001353633.2 | 42.5% | 30.7% | (many diffs) |
| 17 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | XM_017020840.2 | 42.5% | 30.7% | (many diffs) |
| 18 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NR_148478.2 | 16.9% | 1_48del;226_296del;639_3062del | |
| 19 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NR_148476.2 | 14.8% | (many diffs) | |
| 20 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NR_148480.2 | 14.7% | (many diffs) | |
| 21 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NR_148475.2 | 13.9% | (many diffs) | |
| 22 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NR_148477.2 | 13.9% | (many diffs) | |
| 23 | human | 90634 | N4BP2L1 | NEDD4 binding protein 2 like 1 | NR_148479.2 | 13.8% | (many diffs) | |
| 24 | mouse | 100637 | N4bp2l1 | NEDD4 binding protein 2-like 1 | NM_133898.4 | 59.2% | 50.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 585
- ORF length:
- 519
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ggacagtttc cttcaatctt ttgggaggct gagcctccag ccccagcagc 121 agcagcagcg gcagcggccg ccccggccgc ccccgcgggg gacacctcct cgccgccaca 181 gctttaggaa acacctctac ctcctgcgag gcctcccggg ctccgggaaa actacactgg 241 ccagacaatt gcagcatgac tttcccaggg ccctgatttt cagcacggat gattttttct 301 tcagggaaga tggtgcctat gagttcaatc ctgacttcct ggaggaagct catgaatgga 361 accaaaaaag agcaagaaaa gcaatgagga atggcatatc ccccattatt attgataata 421 ccaacctcca cgcctgggaa atgaagccct atgcagtcat ggtatttcag accgaacaaa 481 agaatctttt caggctggaa atggacatgg tagttttcag gccagaaatg aagaaacatt 541 catggtgtct caagagaaaa aatccaccga atgaaagaac ggtatgccca actttcttgt 601 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 661 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a