Construct: ORF ccsbBroad304_05107
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011333.1_s304c1, BRDN0000405260
- Derived from:
- ccsbBroadEn_05107
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMTC3 (160418)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05107
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 160418 | TMTC3 | transmembrane O-mannosyltra... | NM_181783.4 | 100% | 100% | |
| 2 | human | 160418 | TMTC3 | transmembrane O-mannosyltra... | NM_001366574.1 | 93.4% | 93.4% | 0_1ins180 |
| 3 | human | 160418 | TMTC3 | transmembrane O-mannosyltra... | NM_001366579.1 | 92% | 92% | 189_190ins219 |
| 4 | human | 160418 | TMTC3 | transmembrane O-mannosyltra... | NM_001366580.1 | 90.2% | 90.2% | 0_1ins267 |
| 5 | human | 160418 | TMTC3 | transmembrane O-mannosyltra... | NM_001366583.1 | 74.7% | 74.7% | 0_1ins693 |
| 6 | human | 160418 | TMTC3 | transmembrane O-mannosyltra... | NR_159381.1 | 37.4% | 1_209del;1409_1532del;3076_7316del | |
| 7 | mouse | 237500 | Tmtc3 | transmembrane and tetratric... | NM_001110013.1 | 85% | 89.7% | (many diffs) |
| 8 | mouse | 237500 | Tmtc3 | transmembrane and tetratric... | XM_006513630.3 | 85% | 89.7% | (many diffs) |
| 9 | mouse | 237500 | Tmtc3 | transmembrane and tetratric... | XM_011243449.2 | 85% | 89.7% | (many diffs) |
| 10 | mouse | 237500 | Tmtc3 | transmembrane and tetratric... | XM_011243450.2 | 85% | 89.7% | (many diffs) |
| 11 | mouse | 237500 | Tmtc3 | transmembrane and tetratric... | NM_001033332.2 | 61.3% | 65.6% | (many diffs) |
| 12 | mouse | 237500 | Tmtc3 | transmembrane and tetratric... | XM_011243451.1 | 45.3% | 48.3% | (many diffs) |
| 13 | mouse | 237500 | Tmtc3 | transmembrane and tetratric... | XM_017313937.1 | 42.5% | 42.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2811
- ORF length:
- 2742
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctaatatt aacctaaaag aaataacctt aatagtaggt gtggttactg 121 cctgctattg gaacagcctc ttttgtggtt ttgtttttga tgatgtttca gcaatactgg 181 ataacaaaga cttgcatcca tctacacctt taaaaacttt atttcaaaat gacttctggg 241 gaacccctat gtctgaggag agaagccaca agtcttaccg tcccttaaca gtattgacat 301 ttcgcttaaa ttatttgtta agtgaactaa aaccaatgtc atatcatctc ctgaatatga 361 tttttcatgc tgtggttagt gtgatatttc tcaaagtatg caaacttttt ctggacaaca 421 agagtagtgt gattgcttct ttactttttg cagtgcaccc aatacacaca gaagcagtaa 481 caggagttgt tggaagagca gaacttttgt catctatctt ttttctagca gcttttttgt 541 catataccag atcaaaagga ccagacaatt ccataatatg gactccaatt gccttgacag 601 tgtttttagt ggctgttgca acattatgta aagaacaagg aataacagtt gtaggaattt 661 gctgtgtgta tgaagtgttt attgcccagg ggtatacttt gccattacta tgtactactg 721 ctggacagtt tctccgtgga aagggtagca ttccattttc tatgctgcag acactagtaa 781 aactcattgt cttgatgttc agtacattat tacttgttgt gattagagtc caggttattc 841 aatcccaact tccagtattc accaggtttg ataacccagc tgctgtaagc ccaactccta 901 caaggcaact aacttttaac tacctccttc ctgtgaatgc ttggttgtta ttaaatcctt 961 cagagctctg ctgtgattgg accatgggaa caataccact tatagagtca ttactagata 1021 ttcgaaatct ggccacattt actttctttt gttttctggg gatgttggga gtattcagta 1081 tcagatactc tggtgattcc tccaagactg ttttaatggc gctttgttta atggcattac 1141 catttattcc tgcatcgaac cttttttttc cagttggatt tgttgttgcc gagcgagtat 1201 tatatgttcc cagcatgggg ttctgtattt tggtagccca tggatggcag aaaatatcaa 1261 caaaaagtgt atttaaaaag ctatcctgga tttgtctgtc tatggtgata ctcactcatt 1321 ccttaaaaac attccacaga aattgggatt gggagtctga atatacattg tttatgtcag 1381 ccttgaaggt aaataaaaat aatgccaaac tttggaataa tgtgggtcat gctctggaaa 1441 atgaaaagaa ctttgagaga gctttgaaat acttcttaca ggctacccat gttcagccag 1501 atgatattgg tgcccatatg aatgtaggaa gaacttataa aaatttaaat agaaccaaag 1561 aagctgaaga atcttacatg atggctaaat cactgatgcc tcaaattatt cctggtaaaa 1621 aatatgcagc cagaattgcc cctaaccacc taaatgttta tatcaatctg gctaacctga 1681 tccgagcaaa tgagtcccga ctggaagaag cagatcagct gtaccgtcaa gcaataagca 1741 tgaggcccga cttcaagcag gcttacatta gcagaggaga attgctttta aaaatgaata 1801 aacctcttaa agcaaaggaa gcatatctta aagcactaga gctggacaga aataatgcag 1861 atctttggta caacttggca attgtacata ttgaacttaa agaaccaaat gaagccctaa 1921 aaaactttaa tcgtgctctg gaactaaatc caaagcataa actagcatta ttcaactctg 1981 ctatagtaat gcaagaatca ggtgaggtta aactcagacc tgaagctaga aaacgacttc 2041 taagttatat aaatgaagag ccactagatg ctaatgggta tttcaatttg ggaatgcttg 2101 ccatggatga caaaaaggac aatgaagcag agatttggat gaagaaagcc ataaagttac 2161 aagccgactt ccgaagtgct ttgtttaatc tggctctcct gtattcccag actgcaaagg 2221 aattaaaggc tttgccaatt ttggaggagt tactcagata ctaccctgat catatcaagg 2281 gcctcatttt aaaaggagac attctgatga atcaaaagaa agatatacta ggagcaaaaa 2341 aatgttttga aaggattttg gagatggatc caagcaatgt gcaaggaaaa cacaatcttt 2401 gtgttgttta ttttgaagaa aaagacttat taaaagctga aagatgcctt cttgaaacac 2461 tggcattagc accacatgaa gaatatattc agcgccattt gaatatagtc agggataaga 2521 tttcctcatc tagttttata gagccaatat tcccaaccag taagatttca agtgtggaag 2581 gaaagaaaat tccaactgaa agtgtaaaag aaattagagg tgaatccaga caaacacaaa 2641 tagtaaaaac aagtgataat aaaagtcagt ctaaatccaa caaacaatta ggaaaaaatg 2701 gagacgaaga gacaccccac aaaacaacaa aagacatcaa agaaattgag aagaaaagag 2761 ttgctgcttt aaaaagacta gaagagattg aacgtatttt aaatggtgaa ttgccaactt 2821 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 2881 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta