Construct: ORF ccsbBroad304_05186
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000798.1_s304c1, BRDN0000405522
- Derived from:
- ccsbBroadEn_05186
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APOBEC3A (200315)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05186
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100913187 | APOBEC3A_B | APOBEC3A and APOBEC3B delet... | NM_001193289.1 | 100% | 100% | |
| 2 | human | 200315 | APOBEC3A | apolipoprotein B mRNA editi... | NM_145699.4 | 100% | 100% | |
| 3 | human | 200315 | APOBEC3A | apolipoprotein B mRNA editi... | NM_001270406.2 | 90.9% | 90.9% | 27_28ins54 |
| 4 | human | 9582 | APOBEC3B | apolipoprotein B mRNA editi... | NM_004900.5 | 48.5% | 44.7% | (many diffs) |
| 5 | human | 9582 | APOBEC3B | apolipoprotein B mRNA editi... | NM_001270411.2 | 41.9% | 38.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 666
- ORF length:
- 597
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaagccagc ccagcatccg ggcccagaca cttgatggat ccacacatat 121 tcacttccaa ctttaacaat ggcattggaa ggcataagac ctacctgtgc tacgaagtgg 181 agcgcctgga caatggcacc tcggtcaaga tggaccagca caggggcttt ctacacaacc 241 aggctaagaa tcttctctgt ggcttttacg gccgccatgc ggagctgcgc ttcttggacc 301 tggttccttc tttgcagttg gacccggccc agatctacag ggtcacttgg ttcatctcct 361 ggagcccctg cttctcctgg ggctgtgccg gggaagtgcg tgcgttcctt caggagaaca 421 cacacgtgag actgcgtatc ttcgctgccc gcatctatga ttacgacccc ctatataagg 481 aggcactgca aatgctgcgg gatgctgggg cccaagtctc catcatgacc tacgatgaat 541 ttaagcactg ctgggacacc tttgtggacc accagggatg tcccttccag ccctgggatg 601 gactagatga gcacagccaa gccctgagtg ggaggctgcg ggccattctc cagaatcagg 661 gaaacttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 721 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 781 acctctggat ta