Construct: ORF ccsbBroad304_05192

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015964.1_s304c1, BRDN0000412147
Derived from:
ccsbBroadEn_05192
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPRED2 (200734)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05192
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 200734 SPRED2 sprouty related EVH1 domain... NM_181784.3 100% 100%
2 human 200734 SPRED2 sprouty related EVH1 domain... NM_001128210.2 98.5% 98% (many diffs)
3 human 200734 SPRED2 sprouty related EVH1 domain... XM_017003576.1 70.4% 70.3% 0_1ins366;3_3delGinsAGAA
4 human 200734 SPRED2 sprouty related EVH1 domain... XM_005264200.5 54.2% 45.9% (many diffs)
5 human 200734 SPRED2 sprouty related EVH1 domain... XM_005264201.1 52.8% 52.8% 0_1ins591
6 human 200734 SPRED2 sprouty related EVH1 domain... XM_005264202.5 49.4% 47.3% (many diffs)
7 mouse 114716 Spred2 sprouty-related, EVH1 domai... NM_033523.4 86.5% 92.1% (many diffs)
8 mouse 114716 Spred2 sprouty-related, EVH1 domai... XM_006514458.3 85.1% 90.1% (many diffs)
9 mouse 114716 Spred2 sprouty-related, EVH1 domai... XM_006514457.3 84.9% 90% (many diffs)
10 mouse 114716 Spred2 sprouty-related, EVH1 domai... XM_006514456.3 82.6% 86.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1323
ORF length:
1254
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaccgaagaa acacacccag acgatgacag ctatattgtg cgtgtcaagg
121ctgtggttat gaccagagat gactccagcg ggggatggtt cccacaggaa ggaggcggga
181tcagtcgcgt cggggtctgt aaggtcatgc accccgaagg caatggacga agcggctttc
241tcatccatgg tgaacgacag aaagacaaac tggtggtatt ggaatgctat gtaagaaagg
301acttggtcta caccaaagcc aatccaacgt ttcatcactg gaaggtcgat aataggaagt
361ttggacttac tttccaaagc cctgctgatg cccgagcctt tgacagggga gtaaggaaag
421caatcgaaga ccttatagaa ggttcaacaa cgtcatcttc caccatccat aatgaagctg
481agcttggcga tgatgacgtt tttacaacag ctacagacag ttcttctaat tcctctcaga
541agagagagca acctactcgg acaatctcct ctcccacatc ctgtgagcac cggaggattt
601ataccctggg ccacctccac gactcatacc ccacagacca ctatcacctc gatcagccga
661tgccaaggcc ctaccgccag gtgagcttcc cggacgacga cgaggagatc gtgcgcatca
721acccccggga gaagatctgg atgacggggt acgaggatta ccggcacgca cccgtcaggg
781gcaagtaccc ggacccctcg gaggacgcgg actcctccta cgtgcgcttc gccaagggcg
841aggtccccaa gcatgactac aactacccct acgtggactc ctcagacttt ggcctaggcg
901aggaccccaa aggccgcggg ggcagcgtga tcaagacgca gccctcccgg ggcaagtcgc
961ggcggcggaa ggaggacgga gagcgctcgc ggtgcgtgta ctgcagggac atgttcaacc
1021acgaggagaa ccgccggggc cactgccagg acgcgcccga ctccgtgaga acttgcatcc
1081gccgggtgag ctgcatgtgg tgcgcggaca gcatgctcta tcactgtatg tcggaccccg
1141agggagacta tacagaccct tgctcgtgcg atactagcga cgagaagttt tgcctccggt
1201ggatggctct tattgccttg tctttcctgg ccccctgtat gtgctgttac ctgccccttc
1261gggcctgcta ccactgcgga gtgatgtgca ggtgctgtgg cgggaagcac aaagcggccg
1321cgttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
1381tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
1441tctggatta

Download FASTA (ORF) (Full)