Construct: ORF ccsbBroad304_05407
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000734.1_s304c1, BRDN0000403222
- Derived from:
- ccsbBroadEn_05407
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CEP57L1 (285753)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05407
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001083535.2 | 100% | 100% | |
| 2 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001271852.2 | 100% | 100% | |
| 3 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001350652.1 | 100% | 100% | |
| 4 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001350653.1 | 100% | 100% | |
| 5 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_173830.5 | 100% | 100% | |
| 6 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | XM_017010769.1 | 99.3% | 99.3% | 1_9delATGAAGATA |
| 7 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001350654.1 | 96.4% | 96.4% | 823_873del |
| 8 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001350655.1 | 96.4% | 96.4% | 823_873del |
| 9 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001350656.1 | 96.4% | 96.4% | 821_871del |
| 10 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001350657.1 | 96.4% | 96.4% | 823_873del |
| 11 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001350658.1 | 96.4% | 96.4% | 823_873del |
| 12 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | XM_006715462.3 | 96.4% | 96.4% | 823_873del |
| 13 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | XM_017010768.2 | 96.4% | 96.4% | 821_871del |
| 14 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | XM_024446404.1 | 95.8% | 95.8% | 1_9delATGAAGATA;832_882del |
| 15 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001350659.1 | 93% | 93% | 338_339ins48;775_825del |
| 16 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | XM_005266938.4 | 92.3% | 92.3% | 1_114del |
| 17 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | XM_005266935.5 | 89.3% | 89.3% | 1_114del;937_987del |
| 18 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | XM_005266936.4 | 89.3% | 89.3% | 1_114del;935_985del |
| 19 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | XM_005266937.4 | 86.2% | 86.2% | 1_114del;452_453ins48;889_939del |
| 20 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001271853.2 | 84.6% | 84.5% | (many diffs) |
| 21 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | XM_024446405.1 | 84.6% | 84.5% | (many diffs) |
| 22 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | XM_024446406.1 | 81.1% | 81% | (many diffs) |
| 23 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001350662.1 | 70% | 70% | 0_1ins414 |
| 24 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001350663.1 | 70% | 70% | 0_1ins414 |
| 25 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001350664.1 | 70% | 70% | 0_1ins414 |
| 26 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001350660.1 | 67.5% | 67.5% | 0_1ins414;409_459del |
| 27 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001350661.1 | 67.5% | 67.5% | 0_1ins414;409_459del |
| 28 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | XM_011535762.2 | 64.7% | 64.1% | (many diffs) |
| 29 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | XM_017010771.1 | 64.7% | 64.1% | (many diffs) |
| 30 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001350665.1 | 54.2% | 54.3% | 745_745delGinsAAAACTAAA;749_750delGC;753_754ins621 |
| 31 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | XM_011535763.2 | 52.8% | 50.8% | (many diffs) |
| 32 | human | 285753 | CEP57L1 | centrosomal protein 57 like 1 | NM_001350666.1 | 50.7% | 50.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1446
- ORF length:
- 1380
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ttctgaatta atgcatagta tagtaggaag ctatcataaa cctccagaaa 121 gagtatttgt tccctcattc acccagaatg aaccatctca gaattgccat cctgcaaact 181 tagaagttac ctctcctaag atacttcata gcccaaatag ccaagctctt attttagcct 241 taaaaactct tcaggaaaaa attcatcgtt tagagctgga gagaacacaa gctgaagata 301 acctgaacat tctttccaga gaagcagcac agtataagaa ggccttagag aatgaaacaa 361 atgagagaaa tctggcacac caggagctga taaagcagaa aaaagatata agtatacagt 421 taagctcagc ccagtctcgt tgtactcttc tagagaagca actagaatat acaaagagaa 481 tggttctcaa cgtagagcga gaaaagaaca tgatcctaga acaacaggcc cagcttcaga 541 gggaaaaaga acaagatcag atgaagctgt atgcaaaact tgaaaagctt gatgtcttag 601 aaaaagagtg tttcagactt acaacaactc agaaaactgc tgaggacaag attaaacatt 661 tagaagaaaa acttaaggaa gaagaacatc agcgtaagct atttcaagac aaagcttctg 721 agcttcaaac tggacttgaa atcagtaaaa ttataatgtc ttcagtttca aatctaaagc 781 actccaagga aaagaagaaa tcttcaaaga aaactaaatg tataaagaga cgaccacctt 841 ggcaaatttg ttcaaagttt ggagcactgc cttttgtggc tgaaaagatg aggcaacatc 901 gtgacccaca tatccttcag aaacctttta acgtgactga gactagatgt ctccccaagc 961 cttctagaac aacttcctgg tgtaaagcta ttcctcctga ctcagaaaag tccatttcca 1021 tttgtgacaa tttatctgaa cttttgatgg caatgcaaga tgagctggac caaatgagca 1081 tggagcacca agaactactg aaacaaatga aggaaactga aagtcattca gtctgtgacg 1141 acatagaatg tgaactagag tgtttactca agaaaatgga aattaaagga gaacaaatct 1201 ccaaactgaa gaagcatcaa gacagtgtct gtaaactgca gcaaaaagtt caaaactcaa 1261 agatgagtga agcttcaggt attcagcaag aagacagcta ccctaaagga tcaaagaaca 1321 taaaaaatag ccccagaaaa tgtttgactg acactaacct ttttcagaaa aacagcagct 1381 ttcatccaat acgagttcat aatcttcaaa tgaaattgag aagagatgat atcatgtggg 1441 aacagtaaca aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1501 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1561 acctctggat ta