Transcript: Human NM_001271852.2

Homo sapiens centrosomal protein 57 like 1 (CEP57L1), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-02-25
Taxon:
Homo sapiens (human)
Gene:
CEP57L1 (285753)
Length:
6496
CDS:
958..2340

Additional Resources:

NCBI RefSeq record:
NM_001271852.2
NBCI Gene record:
CEP57L1 (285753)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001271852.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000425654 GAACATCAGCGTAAGCTATTT pLKO_005 1576 CDS 100% 13.200 18.480 N CEP57L1 n/a
2 TRCN0000150697 GCTGAGGACAAGATTAAACAT pLKO.1 1531 CDS 100% 5.625 7.875 N CEP57L1 n/a
3 TRCN0000418640 TAGTAGGTTTGGAACCTTAAA pLKO_005 2693 3UTR 100% 13.200 10.560 N CEP57L1 n/a
4 TRCN0000152263 CTAAGATACTTCATAGCCCAA pLKO.1 1088 CDS 100% 2.160 1.728 N CEP57L1 n/a
5 TRCN0000155059 GCAGAAGCTTTCCTCTGCTAT pLKO.1 2924 3UTR 100% 0.495 0.396 N CEP57L1 n/a
6 TRCN0000427845 GACGACCACCTTGGCAAATTT pLKO_005 1721 CDS 100% 15.000 10.500 N CEP57L1 n/a
7 TRCN0000151002 CATCAAGACAGTGTCTGTAAA pLKO.1 2107 CDS 100% 13.200 9.240 N CEP57L1 n/a
8 TRCN0000432742 ATTCAGTCTGTGACGACATAG pLKO_005 2018 CDS 100% 10.800 7.560 N CEP57L1 n/a
9 TRCN0000151881 CCTCTCCTAAGATACTTCATA pLKO.1 1082 CDS 100% 5.625 3.938 N CEP57L1 n/a
10 TRCN0000151396 GCTGAAGATAACCTGAACATT pLKO.1 1183 CDS 100% 5.625 3.938 N CEP57L1 n/a
11 TRCN0000155287 GCCCAAATAGCCAAGCTCTTA pLKO.1 1103 CDS 100% 4.950 2.970 N CEP57L1 n/a
12 TRCN0000155079 GCAAGAAGACAGCTACCCTAA pLKO.1 2178 CDS 100% 4.050 2.430 N CEP57L1 n/a
13 TRCN0000375438 TCAGCCCAGTCTCGTTGTATT pLKO_005 1318 CDS 100% 13.200 18.480 N Cep57l1 n/a
14 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 6010 3UTR 100% 5.625 2.813 Y KLHL30 n/a
15 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 6010 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001271852.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05407 pDONR223 99% 100% 100% None n/a
2 ccsbBroad304_05407 pLX_304 0% 100% 100% V5 (not translated due to prior stop codon) n/a
3 ccsbBroadEn_14466 pDONR223 100% 54.1% 53.6% None (many diffs) n/a
4 ccsbBroad304_14466 pLX_304 0% 54.1% 53.6% V5 (not translated due to frame shift) (many diffs) n/a
5 TRCN0000475203 CGTCGGAGACTTGGCAAAATACGA pLX_317 62.6% 54.1% 53.6% V5 (not translated due to frame shift) (many diffs) n/a
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