Construct: ORF ccsbBroad304_05634
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005885.1_s304c1, BRDN0000460271
- Derived from:
- ccsbBroadEn_05634
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRAPL (400581)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05634
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 400581 | GRAPL | GRB2 related adaptor protei... | NM_001129778.3 | 100% | 100% | |
| 2 | human | 400581 | GRAPL | GRB2 related adaptor protei... | XM_017024637.1 | 100% | 100% | |
| 3 | human | 400581 | GRAPL | GRB2 related adaptor protei... | NM_001353418.2 | 98.3% | 97.4% | 299_300insTAACAG |
| 4 | human | 400581 | GRAPL | GRB2 related adaptor protei... | XM_011523857.3 | 86.7% | 83.3% | (many diffs) |
| 5 | human | 400581 | GRAPL | GRB2 related adaptor protei... | XM_017024635.2 | 77.4% | 71% | (many diffs) |
| 6 | human | 400581 | GRAPL | GRB2 related adaptor protei... | XM_017024636.2 | 77.4% | 71% | (many diffs) |
| 7 | human | 10750 | GRAP | GRB2 related adaptor protein | NM_001330148.2 | 53.6% | 50.7% | (many diffs) |
| 8 | human | 10750 | GRAP | GRB2 related adaptor protein | NM_006613.4 | 48.5% | 47% | (many diffs) |
| 9 | mouse | 71520 | Grap | GRB2-related adaptor protein | NM_027817.3 | 46.1% | 45.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 423
- ORF length:
- 354
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagtccgtg gccctgtaca gctttcaggc tacagagagc gacgagctgg 121 ccttcaacaa gggagacaca ctcaagatcc tgaacatgga ggatgaccag aactggtaca 181 aggccgagct ccggggtgtc gagggattta ttcccaagaa ctacatccgc gtcaagcccc 241 atccgtggta ctcgggcagg atttcccggc agctggccga agagattctg atgaagcgga 301 accatctggg agccttcctg atccgggaga gtgagagctc cccaggggag ttctctgtgt 361 ctgtgaataa cagagcccag aggggtccct gcctgggccc aaaatcacac agcagattgg 421 ggttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 481 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 541 tctggatta