Construct: ORF ccsbBroad304_05732
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012009.1_s304c1, BRDN0000408399
- Derived from:
- ccsbBroadEn_05732
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSAG2 (728461)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05732
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389903 | CSAG3 | CSAG family member 3 | NM_001129828.3 | 100% | 100% | |
| 2 | human | 102723547 | CSAG2 | CSAG family member 2 | XM_017030023.1 | 99.6% | 99% | 187A>G |
| 3 | human | 389903 | CSAG3 | CSAG family member 3 | NM_001129826.3 | 86.6% | 86.6% | 264_314del |
| 4 | human | 389903 | CSAG3 | CSAG family member 3 | XM_024452379.1 | 86.6% | 86.6% | 264_314del |
| 5 | human | 102723547 | CSAG2 | CSAG family member 2 | XM_006724857.3 | 86.3% | 85.8% | 187A>G;264_314del |
| 6 | human | 158511 | CSAG1 | chondrosarcoma associated g... | NM_001102576.3 | 66% | 59% | (many diffs) |
| 7 | human | 158511 | CSAG1 | chondrosarcoma associated g... | NM_153478.3 | 66% | 59% | (many diffs) |
| 8 | human | 102723547 | CSAG2 | CSAG family member 2 | XR_938529.2 | 38% | (many diffs) | |
| 9 | human | 100130935 | CSAG4 | CSAG family member 4 (pseud... | NR_073432.1 | 36.8% | (many diffs) | |
| 10 | human | 389903 | CSAG3 | CSAG family member 3 | NR_130638.1 | 36.5% | (many diffs) | |
| 11 | human | 102723547 | CSAG2 | CSAG family member 2 | XR_938528.2 | 24% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 399
- ORF length:
- 330
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggatgggc ctcatccaat tagttgaagg tgttaagaga aaagaccaag 121 gtttcctgga aaaggaattc taccacaaga ctaacataaa aatgcgctgt gagtttctag 181 cctgctggcc tgccttcact gtcctggggg aggcttggag agaccaggtg gactggagta 241 gactgttgag agacgctggt ctggtgaaga tgtccaggaa accacgagcc tccagcccat 301 tgtccaacaa ccacccacca acaccaaaga ggttcccaag acaacccgga agggaaaagg 361 gacccatcaa ggaagttcca ggaacaaaag gctctccctt gccaactttc ttgtacaaag 421 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 481 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta