Transcript: Human XM_006724857.3

PREDICTED: Homo sapiens CSAG family member 2 (CSAG2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CSAG2 (102723547)
Length:
973
CDS:
280..663

Additional Resources:

NCBI RefSeq record:
XM_006724857.3
NBCI Gene record:
CSAG2 (102723547)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006724857.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000115813 CTACCACAAGACTAACATAAA pLKO.1 351 CDS 100% 13.200 6.600 Y CSAG2 n/a
2 TRCN0000180260 CTCTTCGGACAGCCAGTAATT pLKO.1 804 3UTR 100% 13.200 6.600 Y CSAG3 n/a
3 TRCN0000244713 CTCTTCGGACAGCCAGTAATT pLKO_005 804 3UTR 100% 13.200 6.600 Y CSAG3 n/a
4 TRCN0000244715 TCTACCACAAGACTAACATAA pLKO_005 350 CDS 100% 13.200 6.600 Y CSAG3 n/a
5 TRCN0000163988 CAAGGAAGTTCCAGGAACAAA pLKO.1 630 CDS 100% 5.625 2.813 Y CSAG1 n/a
6 TRCN0000115812 CGAACGAGGAACTCAATCAAA pLKO.1 731 3UTR 100% 5.625 2.813 Y CSAG2 n/a
7 TRCN0000244714 TGGCCCAGAAGCCCTATCAAA pLKO_005 573 CDS 100% 5.625 2.813 Y CSAG3 n/a
8 TRCN0000164670 CTGGTGAAGATGTCCAGGAAA pLKO.1 472 CDS 100% 4.950 2.475 Y CSAG1 n/a
9 TRCN0000183478 GAGACTTCAAGTCTATCTGAA pLKO.1 839 3UTR 100% 4.950 2.475 Y CSAG3 n/a
10 TRCN0000244712 ACACCAAAGAGGCGAGGAAGT pLKO_005 532 CDS 100% 4.050 2.025 Y CSAG3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006724857.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05732 pDONR223 100% 86.3% 85.8% None 187A>G;264_314del n/a
2 ccsbBroad304_05732 pLX_304 0% 86.3% 85.8% V5 187A>G;264_314del n/a
3 TRCN0000469134 GATCCATCCTAGTGGCTCACGGAC pLX_317 100% 86.3% 85.8% V5 187A>G;264_314del n/a
4 ccsbBroadEn_09724 pDONR223 100% 57.5% 51.9% None (many diffs) n/a
5 ccsbBroad304_09724 pLX_304 0% 57.5% 51.9% V5 (many diffs) n/a
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