Construct: ORF ccsbBroad304_05740
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003174.1_s304c1, BRDN0000400898
- Derived from:
- ccsbBroadEn_05740
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GTF2H2C_2 (730394)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05740
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 730394 | GTF2H2C_2 | GTF2H2 family member C, copy 2 | NM_001042490.4 | 100% | 100% | |
| 2 | human | 730394 | GTF2H2C_2 | GTF2H2 family member C, copy 2 | NM_001354437.1 | 100% | 100% | |
| 3 | human | 730394 | GTF2H2C_2 | GTF2H2 family member C, copy 2 | NM_001354438.1 | 100% | 100% | |
| 4 | human | 728340 | GTF2H2C | GTF2H2 family member C | NM_001098728.2 | 99.9% | 100% | 553C>T |
| 5 | human | 728340 | GTF2H2C | GTF2H2 family member C | XM_005248588.4 | 99.9% | 100% | 553C>T |
| 6 | human | 728340 | GTF2H2C | GTF2H2 family member C | XM_005248590.3 | 99.9% | 100% | 553C>T |
| 7 | human | 728340 | GTF2H2C | GTF2H2 family member C | XM_006714688.4 | 99.9% | 100% | 553C>T |
| 8 | human | 2966 | GTF2H2 | general transcription facto... | NM_001364567.1 | 99.5% | 99.2% | (many diffs) |
| 9 | human | 2966 | GTF2H2 | general transcription facto... | NM_001515.3 | 99.5% | 99.2% | (many diffs) |
| 10 | human | 2966 | GTF2H2 | general transcription facto... | XM_017009403.2 | 99.5% | 99.2% | (many diffs) |
| 11 | human | 2966 | GTF2H2 | general transcription facto... | XM_017009404.1 | 99.5% | 99.2% | (many diffs) |
| 12 | human | 2966 | GTF2H2 | general transcription facto... | XM_017009406.2 | 99.5% | 99.2% | (many diffs) |
| 13 | human | 728340 | GTF2H2C | GTF2H2 family member C | XM_011543615.1 | 95.4% | 84.5% | 553C>T;1028_1029ins40;1146_1158del |
| 14 | human | 2966 | GTF2H2 | general transcription facto... | NM_001364568.1 | 89.5% | 85.4% | (many diffs) |
| 15 | human | 2966 | GTF2H2 | general transcription facto... | NM_001364569.1 | 89.5% | 85.4% | (many diffs) |
| 16 | human | 2966 | GTF2H2 | general transcription facto... | NM_001364570.1 | 89.5% | 85.4% | (many diffs) |
| 17 | human | 2966 | GTF2H2 | general transcription facto... | NM_001364571.1 | 89.5% | 85.4% | (many diffs) |
| 18 | human | 728340 | GTF2H2C | GTF2H2 family member C | XM_005248592.3 | 85.7% | 85.8% | 0_1ins168;385C>T |
| 19 | human | 730394 | GTF2H2C_2 | GTF2H2 family member C, copy 2 | NM_001354439.1 | 85.5% | 85.5% | 0_1ins171 |
| 20 | human | 728340 | GTF2H2C | GTF2H2 family member C | XM_017009804.1 | 85.4% | 85.5% | 0_1ins171;382C>T |
| 21 | human | 2966 | GTF2H2 | general transcription facto... | XM_024446037.1 | 85.1% | 84.8% | (many diffs) |
| 22 | human | 2966 | GTF2H2 | general transcription facto... | NM_001364572.1 | 80.8% | 70.1% | (many diffs) |
| 23 | human | 2966 | GTF2H2 | general transcription facto... | NM_001364573.1 | 75.3% | 71.3% | (many diffs) |
| 24 | human | 653238 | GTF2H2B | general transcription facto... | NR_033417.1 | 25.9% | (many diffs) | |
| 25 | mouse | 23894 | Gtf2h2 | general transcription facto... | NM_022011.4 | 90.4% | 96.7% | (many diffs) |
| 26 | mouse | 23894 | Gtf2h2 | general transcription facto... | NM_001360706.1 | 89.6% | 95.9% | (many diffs) |
| 27 | mouse | 23894 | Gtf2h2 | general transcription facto... | XM_006517662.3 | 89.6% | 95.9% | (many diffs) |
| 28 | mouse | 23894 | Gtf2h2 | general transcription facto... | XR_873759.3 | 66.6% | (many diffs) | |
| 29 | mouse | 23894 | Gtf2h2 | general transcription facto... | NR_153794.1 | 64.1% | (many diffs) | |
| 30 | mouse | 23894 | Gtf2h2 | general transcription facto... | XR_873756.3 | 63.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1251
- ORF length:
- 1185
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga tgaagaacct gaaagaacta agcgatggga aggaggctat gaaagaacat 121 gggagattct taaagaagat gaatctggat cacttaaagc tacaatagaa gacattctat 181 tcaaggcaaa gagaaaaaga gtatttgagc accatggaca agttcgactt ggaatgatgc 241 gccaccttta tgtggtagta gatggatcaa gaacaatgga agaccaagat ttaaagccta 301 atagactgac gtgtacttta aagttgttgg aatactttgt agaggaatat tttgatcaaa 361 atcctattag tcagattgga ataattgtaa ctaagagtaa aagagctgaa aaattgactg 421 aactttcagg aaacccaaga aaacatataa cgtctttgaa ggaagctgtg gatatgacct 481 gccatggaga gccatctctt tataattccc taagcatggc tatgcagact ctaaaacaca 541 tgcctggaca tacaagtcga gaagtactaa tcatctttag cagccttaca acttgcgatc 601 catctaatat ttatgattta atcaagaccc taaaggcagc taaaattaga gtatctgtta 661 ttggattgtc tgcagaagtt cgcgtttgca ctgtacttgc tcgtgaaact ggtggcacgt 721 accatgttat tttagatgaa agccattaca aagagttgct cacacatcat cttagtcctc 781 ctcctgctag ctcaagttct gaatgctcac ttattcgtat gggatttcct cagcacacca 841 ttgcttcttt atctgaccag gatgcaaaac cctctttcag catggcgcat ttggatggca 901 atactgagcc agggcttaca ttaggaggct atttctgccc acagtgtcgg gcaaagtact 961 gtgagctacc tgttgaatgt aaaatctgtg gtcttacttt ggtgtctgct ccccacttgg 1021 cacggtctta ccatcatttg tttcctttgg atgcttttca agaaattccc ctagaagaat 1081 ataatggaga aagattttgt tatggatgtc agggggaatt gaaagaccaa catgtttatg 1141 tttgtgctgt gtgccaaaat gttttctgtg tggactgtga tgtttttgtt catgattctc 1201 tacactgttg ccctggctgt attcataaga ttccagctcc ttcaggtgtt tgcccaactt 1261 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1321 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta