Construct: ORF ccsbBroad304_05830

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010880.1_s304c1, BRDN0000461760
Derived from:
ccsbBroadEn_05830
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
APBA2 (321)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05830
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 321 APBA2 amyloid beta precursor prot... NM_001130414.1 99.9% 99.8% 932T>C;1773C>T
2 human 321 APBA2 amyloid beta precursor prot... NM_001353792.2 99.9% 99.8% 932T>C;1773C>T
3 human 321 APBA2 amyloid beta precursor prot... NM_001353793.2 99.9% 99.8% 932T>C;1773C>T
4 human 321 APBA2 amyloid beta precursor prot... NM_001353794.2 99.9% 99.8% 932T>C;1773C>T
5 human 321 APBA2 amyloid beta precursor prot... XM_024449909.1 99.6% 99.5% 932T>C;1214_1219delAGGTAG;1779C>T
6 human 321 APBA2 amyloid beta precursor prot... NM_001353788.2 98.3% 98.2% 932T>C;1215_1250del;1809C>T
7 human 321 APBA2 amyloid beta precursor prot... NM_001353789.2 98.3% 98.2% 932T>C;1215_1250del;1809C>T
8 human 321 APBA2 amyloid beta precursor prot... NM_001353790.2 98.3% 98.2% 932T>C;1215_1250del;1809C>T
9 human 321 APBA2 amyloid beta precursor prot... NM_001353791.2 98.3% 98.2% 932T>C;1215_1250del;1809C>T
10 human 321 APBA2 amyloid beta precursor prot... NM_005503.3 98.3% 98.2% 932T>C;1215_1250del;1809C>T
11 human 321 APBA2 amyloid beta precursor prot... XM_011521488.3 98.3% 98.2% 932T>C;1215_1250del;1809C>T
12 human 321 APBA2 amyloid beta precursor prot... XM_011521489.2 98.3% 98.2% 932T>C;1215_1250del;1809C>T
13 human 321 APBA2 amyloid beta precursor prot... XM_011521490.2 98.3% 98.2% 932T>C;1215_1250del;1809C>T
14 human 321 APBA2 amyloid beta precursor prot... XM_011521491.1 98.3% 98.2% 932T>C;1215_1250del;1809C>T
15 human 321 APBA2 amyloid beta precursor prot... XM_011521492.2 98.3% 98.2% 932T>C;1215_1250del;1809C>T
16 human 321 APBA2 amyloid beta precursor prot... XM_017022110.1 98.3% 98.2% 932T>C;1215_1250del;1809C>T
17 human 321 APBA2 amyloid beta precursor prot... XM_017022112.2 98.3% 98.2% 932T>C;1215_1250del;1809C>T
18 human 321 APBA2 amyloid beta precursor prot... NM_001353795.2 90.7% 89.2% (many diffs)
19 human 321 APBA2 amyloid beta precursor prot... NM_001353797.2 58.8% 57.6% (many diffs)
20 human 321 APBA2 amyloid beta precursor prot... NM_001353796.2 57.9% 56.7% (many diffs)
21 mouse 11784 Apba2 amyloid beta (A4) precursor... XM_006540576.3 87.6% 92.4% (many diffs)
22 mouse 11784 Apba2 amyloid beta (A4) precursor... NM_001291166.1 86.2% 90.9% (many diffs)
23 mouse 11784 Apba2 amyloid beta (A4) precursor... NM_001291167.1 86.2% 90.9% (many diffs)
24 mouse 11784 Apba2 amyloid beta (A4) precursor... NM_007461.2 86.2% 90.9% (many diffs)
25 mouse 11784 Apba2 amyloid beta (A4) precursor... XM_006540573.3 86.2% 90.9% (many diffs)
26 mouse 11784 Apba2 amyloid beta (A4) precursor... XM_006540574.3 86.2% 90.9% (many diffs)
27 mouse 11784 Apba2 amyloid beta (A4) precursor... XM_017321945.1 86.2% 90.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2280
ORF length:
2211
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcccaccgg aagcttgaga gcgtggggag cggcatgttg gaccataggg
121tgagaccagg tcctgtccct cacagccagg agcccgagag cgaggacatg gagctgccct
181tggagggcta tgtgcccgag ggcctggagc tggctgccct gcggccagag agccccgcgc
241cagaggaaca ggagtgccac aaccacagcc ccgatgggga ctccagctct gactacgtga
301acaacacctc tgaggaggag gactatgacg agggcctccc tgaggaggag gagggcatca
361cctactacat ccgctactgc cctgaggacg acagctacct agagggcatg gactgcaacg
421gggaggagta cctggcccac agtgcacacc ctgtggacac tgatgagtgc caggaggcgg
481tggaggagtg gacggactcg gcgggcccgc acccccacgg ccacgaggct gaaggcagcc
541aggactaccc agacggccaa ctgcccattc cggaggatga gccctccgtc cttgaggccc
601atgaccagga agaagatggt cactactgtg ccagcaaaga gggctaccag gactactacc
661ccgaggaggc caacgggaac accggcgcct ccccctaccg cctgaggcgt ggggatgggg
721acctggagga ccaggaggag gacattgacc agatcgtggc agagatcaag atgagtctga
781gcatgaccag catcaccagc gccagtgagg ccagccccga gcatgggcct gagccagggc
841ctgaggactc tgtagaggcc tgcccaccca tcaaggccag ctgcagcccc agcaggcacg
901aggcgaggcc caagtcgctg aacctccttc ccgaggccaa gcaccccgga gacccccaga
961gaggcttcaa gcccaagacc aggaccccag aagagaggcc gaagtggccc cacgagcagg
1021tttgcaatgg tctggagcag ccaaggaagc agcagcgctc tgatctcaat ggacctgttg
1081acaataacaa cattccagag acaaagaagg tggcatcatt tccaagtttt gtggctgttc
1141cagggccctg cgaaccagaa gacctcatcg acgggatcat ctttgctgcc aattacctgg
1201ggtccaccca gctgctatca gaacggaacc cttccaaaaa catcagaatg atgcaagcgc
1261aggaggccgt cagccgggtc aagaattctg agggggatgc ccagacgctg acggaagtgg
1321acctcttcat ttccacccag aggatcaagg ttttaaatgc agacacgcag gaaaccatga
1381tggaccacgc cttgcgtacc atctcctaca tcgccgacat tgggaacatt gtagtgctga
1441tggccagacg ccgcatgccc cggtcagcct ctcaggactg catcgagacc acgcccgggg
1501cccaggaagg caagaagcag tataagatga tctgccatgt gttcgagtcg gaggatgccc
1561agctcatcgc ccagtctatc ggccaggcct tcagcgtggc ctaccaggag ttcctgcgag
1621ccaatggcat caaccccgaa gacttgagcc agaaggaata cagcgacatc atcaacaccc
1681aggagatgta caacgacgac ctcatccact tctcaaactc ggagaactgc aaggagctgc
1741agctggagaa gcacaagggc gagatcctgg gcgtggtggt ggtggagtcg ggctggggct
1801ccatcctgcc cacggtgatc ctggccaaca tgatgaatgg tggcccggct gcccgctcgg
1861ggaagctgag catcggggac cagatcatgt ccatcaatgg caccagcctg gtggggctgc
1921ccctcgccac ctgccaaggc atcatcaagg gcctgaagaa ccagacacag gtgaagctca
1981acattgtcag ctgtcccccg gtcaccacgg tccttatcaa gcggccagac ctcaagtacc
2041agctgggctt cagcgtgcag aatggaatta tctgcagcct catgagaggg ggcattgctg
2101agcgaggggg cgtccgtgtg ggccaccgca tcatcgagat caacgggcag agcgtggtgg
2161ccacagccca cgagaagata gtccaagctc tgtccaactc ggtcggagag atccacatga
2221agaccatgcc cgccgccatg ttcaggctcc tcacgggtca ggagaccccg ctgtacatct
2281tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
2341attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
2401ggatta

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