Construct: ORF ccsbBroad304_05865
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018168.1_s304c1, BRDN0000407236
- Derived from:
- ccsbBroadEn_05865
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP1A3 (478)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05865
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 478 | ATP1A3 | ATPase Na+/K+ transporting ... | NM_152296.5 | 99.9% | 100% | 666T>G |
| 2 | human | 478 | ATP1A3 | ATPase Na+/K+ transporting ... | NM_001256213.1 | 98.8% | 98.7% | 6_38del;699T>G |
| 3 | human | 478 | ATP1A3 | ATPase Na+/K+ transporting ... | NM_001256214.2 | 98.7% | 98.5% | 6_44del;705T>G |
| 4 | human | 477 | ATP1A2 | ATPase Na+/K+ transporting ... | NM_000702.4 | 80.8% | 86.9% | (many diffs) |
| 5 | mouse | 232975 | Atp1a3 | ATPase, Na+/K+ transporting... | NM_001290469.1 | 90.8% | 99.6% | (many diffs) |
| 6 | mouse | 232975 | Atp1a3 | ATPase, Na+/K+ transporting... | XM_011250520.2 | 89.7% | 98.1% | (many diffs) |
| 7 | mouse | 232975 | Atp1a3 | ATPase, Na+/K+ transporting... | XM_011250519.2 | 87.2% | 95.2% | (many diffs) |
| 8 | mouse | 232975 | Atp1a3 | ATPase, Na+/K+ transporting... | XM_011250518.2 | 86.1% | 93.9% | (many diffs) |
| 9 | mouse | 98660 | Atp1a2 | ATPase, Na+/K+ transporting... | NM_178405.3 | 79.1% | 86.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3105
- ORF length:
- 3039
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ggacaagaaa gatgacaagg actcacccaa gaagaacaag ggcaaggagc 121 gccgggacct ggatgacctc aagaaggagg tggctatgac agagcacaag atgtcagtgg 181 aagaggtctg ccggaaatac aacacagact gtgtgcaggg tttgacccac agcaaagccc 241 aggagatcct ggcccgggat gggcctaacg cactcacgcc accgcctacc accccagagt 301 gggtcaagtt ttgccggcag ctcttcgggg gcttctccat cctgctgtgg atcggggcta 361 tcctctgctt cctggcctac ggtatccagg cgggcaccga ggacgacccc tctggtgaca 421 acctgtacct gggcatcgtg ctggcggccg tggtgatcat cactggctgc ttctcctact 481 accaggaggc caagagctcc aagatcatgg agtccttcaa gaacatggtg ccccagcaag 541 ccctggtgat ccgggaaggt gagaagatgc aggtgaacgc tgaggaggtg gtggtcgggg 601 acctggtgga gatcaagggt ggagaccgag tgccagctga cctgcggatc atctcagccc 661 acggctgcaa ggtggacaac tcctccctga ctggcgaatc cgagccccag actcgctctc 721 ccgactgcac gcacgacaac cccttggaga ctcggaacat caccttcttt tccaccaact 781 gtgtggaagg cacggctcgg ggcgtggtgg tggccacggg cgaccgcact gtcatgggcc 841 gtatcgccac cctggcatca gggctggagg tgggcaagac gcccatcgcc atcgagattg 901 agcacttcat ccagctcatc accggcgtgg ctgtcttcct gggtgtctcc ttcttcatcc 961 tctccctcat tctcggatac acctggcttg aggctgtcat cttcctcatc ggcatcatcg 1021 tggccaatgt cccagagggt ctgctggcca ctgtcactgt gtgtctgacg ctgaccgcca 1081 agcgcatggc ccggaagaac tgcctggtga agaacctgga ggctgtagaa accctgggct 1141 ccacgtccac catctgctca gataagacag ggaccctcac tcagaaccgc atgacagtcg 1201 cccacatgtg gtttgacaac cagatccacg aggctgacac cactgaggac cagtcaggga 1261 cctcatttga caagagttcg cacacctggg tggccctgtc tcacatcgct gggctctgca 1321 atcgcgctgt cttcaagggt ggtcaggaca acatccctgt gctcaagagg gatgtggctg 1381 gggatgcgtc tgagtctgcc ctgctcaagt gcatcgagct gtcctctggc tccgtgaagc 1441 tgatgcgtga acgcaacaag aaagtggctg agattccctt caattccacc aacaaatacc 1501 agctctccat ccatgagacc gaggacccca acgacaaccg atacctgctg gtgatgaagg 1561 gtgcccccga gcgcatcctg gaccgctgct ccaccatcct gctacagggc aaggagcagc 1621 ctctggacga ggaaatgaag gaggccttcc agaatgccta ccttgagctc ggtggcctgg 1681 gcgagcgcgt gcttggtttc tgccattatt acctgcccga ggagcagttc cccaagggct 1741 ttgccttcga ctgtgatgac gtgaacttca ccacggacaa cctctgcttt gtgggcctca 1801 tgtccatgat cgacccaccc cgggcagccg tccctgacgc ggtgggcaag tgtcgcagcg 1861 caggcatcaa ggtcatcatg gtcaccggcg atcaccccat cacggccaag gccattgcca 1921 agggtgtggg catcatctct gagggcaacg agactgtgga ggacatcgcc gcccggctca 1981 acattcccgt cagccaggtt aacccccggg atgccaaggc ctgcgtgatc cacggcaccg 2041 acctcaagga cttcacctcc gagcaaatcg acgagatcct gcagaatcac accgagatcg 2101 tcttcgcccg cacatccccc cagcagaagc tcatcattgt ggagggctgt cagagacagg 2161 gtgcaattgt ggctgtgacc ggggatggtg tgaacgactc ccccgctctg aagaaggccg 2221 acattggggt ggccatgggc atcgctggct ctgacgtctc caagcaggca gctgacatga 2281 tcctgctgga cgacaacttt gcctccatcg tcacaggggt ggaggagggc cgcctgatct 2341 tcgacaacct aaagaagtcc attgcctaca ccctgaccag caatatcccg gagatcacgc 2401 ccttcctgct gttcatcatg gccaacatcc cgctgcccct gggcaccatc accatcctct 2461 gcatcgatct gggcactgac atggtccctg ccatctcact ggcgtacgag gctgccgaaa 2521 gcgacatcat gaagagacag cccaggaacc cgcggacgga caaattggtc aatgagagac 2581 tcatcagcat ggcctacggg cagattggaa tgatccaggc tctcggtggc ttcttctctt 2641 actttgtgat cctggcagaa aatggcttct tgcccggcaa cctggtgggc atccggctga 2701 actgggatga ccgcaccgtc aatgacctgg aagacagtta cgggcagcag tggacatacg 2761 agcagaggaa ggtggtggag ttcacctgcc acacggcctt ctttgtgagc atcgttgtcg 2821 tccagtgggc cgatctgatc atctgcaaga cccggaggaa ctcggtcttc cagcagggca 2881 tgaagaacaa gatcctgatc ttcgggctgt ttgaggagac ggccctggct gccttcctgt 2941 cctactgccc cggcatggac gtggccctgc gcatgtaccc tctcaagccc agctggtggt 3001 tctgtgcctt cccctacagt ttcctcatct tcgtctacga cgaaatccgc aaactcatcc 3061 tgcgcaggaa cccagggggt tgggtggaga aggaaaccta ctactgccca actttcttgt 3121 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 3181 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a