Construct: ORF ccsbBroad304_05924

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006504.1_s304c1, BRDN0000461995
Derived from:
ccsbBroadEn_05924
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CACNB2 (783)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05924
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 783 CACNB2 calcium voltage-gated chann... NM_201596.3 99.9% 99.8% 1823G>A
2 human 783 CACNB2 calcium voltage-gated chann... NM_201571.4 95% 93.9% (many diffs)
3 human 783 CACNB2 calcium voltage-gated chann... NM_201597.3 94.8% 93.5% (many diffs)
4 human 783 CACNB2 calcium voltage-gated chann... NM_201593.3 94% 93.6% (many diffs)
5 human 783 CACNB2 calcium voltage-gated chann... NM_201570.3 91.2% 90.3% (many diffs)
6 human 783 CACNB2 calcium voltage-gated chann... NM_000724.4 90.9% 90% (many diffs)
7 human 783 CACNB2 calcium voltage-gated chann... NM_201590.3 90.6% 88.5% (many diffs)
8 human 783 CACNB2 calcium voltage-gated chann... XM_006717502.3 90.1% 89.5% (many diffs)
9 human 783 CACNB2 calcium voltage-gated chann... NM_201572.4 89.8% 87.6% (many diffs)
10 human 783 CACNB2 calcium voltage-gated chann... NM_001167945.2 89.1% 87.7% (many diffs)
11 human 783 CACNB2 calcium voltage-gated chann... XM_011519659.2 85.5% 82.3% (many diffs)
12 human 783 CACNB2 calcium voltage-gated chann... XM_005252588.4 85.3% 84% (many diffs)
13 human 783 CACNB2 calcium voltage-gated chann... NM_001330060.2 85% 83.7% (many diffs)
14 human 783 CACNB2 calcium voltage-gated chann... XM_005252591.3 57.5% 57.4% 0_1ins840;983G>A
15 human 783 CACNB2 calcium voltage-gated chann... XM_017016625.1 57.5% 57.4% 0_1ins840;983G>A
16 human 783 CACNB2 calcium voltage-gated chann... XR_001747198.1 22.7% (many diffs)
17 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497313.3 89.8% 93.6% (many diffs)
18 mouse 12296 Cacnb2 calcium channel, voltage-de... NM_023116.4 89.6% 93.4% (many diffs)
19 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497315.3 86% 89.3% (many diffs)
20 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497314.3 85.8% 89.2% (many diffs)
21 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497318.3 84% 87.4% (many diffs)
22 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497317.3 83.8% 87.2% (many diffs)
23 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497319.3 82.6% 85.6% (many diffs)
24 mouse 12296 Cacnb2 calcium channel, voltage-de... NM_001309519.1 82.4% 85.4% (many diffs)
25 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497320.1 82.1% 85.1% (many diffs)
26 mouse 12296 Cacnb2 calcium channel, voltage-de... NM_001252533.1 82.1% 84.8% (many diffs)
27 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497321.3 80.2% 83.1% (many diffs)
28 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_011238946.2 76.3% 78.9% (many diffs)
29 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497323.1 71.6% 74.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2049
ORF length:
1980
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggtccaaagg gacatgtcca agtcgcctcc cacagcggcg gcggcggtgg
121cgcaggagat ccagatggaa ctgctagaga acgtggctcc cgcgggggcg ctcggagccg
181ccgcacagtc atatggaaaa ggagccagaa ggaaaaacag atttaaagga tctgatggaa
241gcacgtcatc tgatactacc tcaaatagtt ttgttcgcca gggttcggca gactcctaca
301ctagccgtcc atccgattcc gatgtatctc tggaggagga ccgggaggca gtgcgcagag
361aagcggagcg gcaggcccag gcacagttgg aaaaagcaaa gacaaagccc gttgcatttg
421cggttcggac aaatgtcagc tacagtgcgg cccatgaaga tgatgttcca gtgcctggca
481tggccatctc attcgaagca aaagattttc tgcatgttaa ggaaaaattt aacaatgact
541ggtggatagg gcgattggta aaagaaggct gtgaaatcgg attcattcca agcccagtca
601aactagaaaa catgaggctg cagcatgaac agagagccaa gcaagggaaa ttctactcca
661gtaaatcagg aggaaattca tcatccagtt tgggtgacat agtacctagt tccagaaaat
721caacacctcc atcatctgct atagacatag atgctactgg cttagatgca gaagaaaatg
781atattccagc aaaccaccgc tcccctaaac ccagtgcaaa cagtgtaacg tcaccccact
841ccaaagagaa aagaatgccc ttctttaaga agacagagca cactcctccg tatgatgtgg
901taccttccat gcgaccagtg gtcctagtgg gcccttctct gaagggctac gaggtcacag
961atatgatgca aaaagcgctg tttgattttt taaaacacag atttgaaggg cggatatcca
1021tcacaagggt caccgctgac atctcgcttg ccaaacgctc ggtattaaac aatcccagta
1081agcacgcaat aatagaaaga tccaacacaa ggtcaagctt agcggaagtt cagagtgaaa
1141tcgaaaggat ttttgaactt gcaagaacat tgcagttggt ggtccttgac gcggatacaa
1201ttaatcatcc agctcaactc agtaaaacct ccttggcccc tattatagta tatgtaaaga
1261tttcttctcc taaggtttta caaaggttaa taaaatctcg agggaaatct caagctaaac
1321acctcaacgt ccagatggta gcagctgata aactggctca gtgtcctcca gagctgttcg
1381atgtgatctt ggatgagaac cagcttgagg atgcctgtga gcaccttgcc gactatctgg
1441aggcctactg gaaggccacc catcctccca gcagtagcct ccccaaccct ctccttagcc
1501gtacattagc cacttcaagt ctgcctctta gccccaccct agcctctaat tcacagggtt
1561ctcaaggtga tcagaggact gatcgctccg ctcctatccg ttctgcttcc caagctgaag
1621aagaacctag tgtggaacca gtcaagaaat cccagcaccg ctcttcctcc tcagccccac
1681accacaacca tcgcagtggg acaagtcgcg gcctctccag gcaagagaca tttgactcgg
1741aaacccagga gagtcgagac tctgcctacg tagagccaaa ggaagattat tcccatgacc
1801acgtggacca ctatgcctca caccgtgacc acaaccacag agacgagacc cacgggagca
1861gtgaccacag acacagggag tcccggcacc attcccggga cgtggatcga gagcaggacc
1921acaacgagtg caacaagcag cgcagccgtc ataaatccaa ggatcgctac tgtgaaaagg
1981atggagaagt gatatcaaaa aaacggaatg aggctgggga gtggaacagg gatgtttaca
2041tccgccaatt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
2101tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
2161tcaacctctg gatta

Download FASTA (ORF) (Full)