Construct: ORF ccsbBroad304_06083

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016933.1_s304c1, BRDN0000409621
Derived from:
ccsbBroadEn_06083
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DBN1 (1627)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06083
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1627 DBN1 drebrin 1 NM_004395.3 99.8% 99.8% 318G>A;1336A>G
2 human 1627 DBN1 drebrin 1 NM_080881.2 97% 94.4% (many diffs)
3 human 1627 DBN1 drebrin 1 XM_017009140.1 97% 94.2% (many diffs)
4 human 1627 DBN1 drebrin 1 NM_001363541.1 93.2% 93.2% 318G>A;956_1093del;1474A>G
5 human 1627 DBN1 drebrin 1 NM_001364151.1 93.1% 93% (many diffs)
6 human 1627 DBN1 drebrin 1 XM_011534446.2 90.6% 88.1% (many diffs)
7 human 1627 DBN1 drebrin 1 XM_017009139.1 90.6% 88.1% (many diffs)
8 human 1627 DBN1 drebrin 1 XM_011534447.2 90.4% 88.8% (many diffs)
9 human 1627 DBN1 drebrin 1 XM_006714826.2 84.1% 84% (many diffs)
10 human 1627 DBN1 drebrin 1 NM_001364152.1 58.6% 58.5% 0_1ins804;532A>G
11 mouse 56320 Dbn1 drebrin 1 NM_019813.4 84.9% 87.5% (many diffs)
12 mouse 56320 Dbn1 drebrin 1 NM_001177372.1 84.8% 87.4% (many diffs)
13 mouse 56320 Dbn1 drebrin 1 NM_001177371.1 79.4% 81.8% (many diffs)
14 mouse 56320 Dbn1 drebrin 1 XM_006517305.4 79.3% 81.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2013
ORF length:
1947
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc cggcgtcagc ttcagcggcc accgcctgga gctgctggcg gcttacgagg
121aggtgatccg agaggagagc gcggccgact gggctctgta cacatatgaa gatggctccg
181atgacctcaa gcttgcagca tcaggagaag ggggcttgca ggagctttcg ggacactttg
241agaaccagaa ggtgatgtac ggcttctgca gtgtcaagga ctcccaagct gctctgccaa
301aatacgtgct catcaactgg gtgggcgaag atgtgcctga tgcccgcaag tgcgcttgtg
361ccagccacgt ggctaaggtg gcagagttct tccagggtgt cgacgtgatc gtgaacgcca
421gcagcgtgga agacatagac gcgggtgcca tcgggcagcg gctctctaac gggctggcgc
481gactctccag ccctgtgctg caccgactgc ggctgcgaga ggatgagaac gcagagcccg
541tgggcaccac ctaccagaag acggatgcag ctgtggaaat gaagcggatt aaccgagagc
601agttctggga gcaggccaag aaggaagaag agctgcggaa ggaggaggag cggaagaagg
661ccctggatga gaggctcagg ttcgagcagg agcggatgga gcaggagcgg caggagcaag
721aggagcgcga gcggcgctac cgggagcggg agcagcagat cgaggagcac aggaggaaac
781agcagacttt agaagcggaa gaggccaaga ggcggttgaa ggagcagtct atctttggtg
841accatcggga tgaggaggaa gagacccaca tgaagaagtc agagtcggag gtggaggagg
901cagcagctat tattgcccag cggcctgaca acccaaggga gttcttcaag cagcaggaaa
961gagtcgcatc ggcctctgcg ggcagctgtg atgtaccctc gcccttcaac catcgaccag
1021gcagccacct ggacagccac cggaggatgg cgcccactcc catccccacg cggagcccgt
1081ctgactccag caccgcctcc acccctgtcg ctgagcagat agagcgggcc ctggatgagg
1141tcacctcctc gcagcctcca ccactgccac cgccaccccc accagcccaa gagacccagg
1201agcccagccc catcctagac agtgaggaga ccagagcagc agcccctcag gcctgggccg
1261gccccatgga ggagccccct caggcacagg cgcctccccg ggggccaggc agccctgcag
1321aggacttgat gttcatggag tctgcagagc aggctgtcct ggctgctccc gtggagcctg
1381ccacagctga cgccacggag gtccacgatg cagctgacac cattgaaact gacactgcca
1441ctgctgacac cactgttgcc aacaacgtac cccccgccgc caccagcctc attgacctat
1501ggcctggcaa cggggaaggg gcctccacac tccagggtga gcccagggcc cccacgccac
1561cctcgggtac tgaggtcacc ctggcagagg tgcccctgct ggatgaggtg gctccggagc
1621cactgctgcc agcaggcgaa ggctgtgcca cccttctcaa ctttgatgag ctgcctgagc
1681cgccagccac cttctgtgac ccagaggaag tggaagggga gcccctggct gccccccaga
1741ccccaactct gccctcagcc cttgaggagc tggagcaaga gcaggagccg gagccccacc
1801tgctaaccaa tggcgagacc acccagaagg aggggaccca ggccagtgag gggtacttca
1861gtcaatcaca ggaggaggag tttgcccaat cggaagagct ctgtgccaag gctccgcctc
1921ctgtgttcta caacaagcct ccagagatcg acatcacatg ctgggatgca gacccagttc
1981cagaagagga ggagggcttc gagggtggtg attacccaac tttcttgtac aaagtggttg
2041gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
2101accggtggcg cgttaagtcg acaatcaacc tctggatta

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