Construct: ORF ccsbBroad304_06100

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010616.1_s304c1, BRDN0000399067
Derived from:
ccsbBroadEn_06100
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DLST (1743)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06100
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1743 DLST dihydrolipoamide S-succinyl... NM_001933.5 99.9% 100% 576G>A
2 human 1743 DLST dihydrolipoamide S-succinyl... XR_001750184.2 79.8% (many diffs)
3 human 1743 DLST dihydrolipoamide S-succinyl... NR_045209.2 46.5% (many diffs)
4 human 1743 DLST dihydrolipoamide S-succinyl... NR_033814.2 46.2% (many diffs)
5 human 1744 DLSTP1 dihydrolipoamide S-succinyl... NR_130749.1 43.2% (many diffs)
6 human 1743 DLST dihydrolipoamide S-succinyl... NM_001244883.2 33.7% 32.7% (many diffs)
7 mouse 78920 Dlst dihydrolipoamide S-succinyl... NM_030225.4 88.7% 92% (many diffs)
8 mouse 78920 Dlst dihydrolipoamide S-succinyl... XM_006516392.3 88.5% 91.8% (many diffs)
9 mouse 78920 Dlst dihydrolipoamide S-succinyl... XM_017315247.1 53.6% 56.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1425
ORF length:
1359
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct gtcccgatcc cgctgtgtgt ctcgggcgtt cagccgctcg ctctccgcct
121tccagaaggg gaactgccct ctagggagac gttccctgcc tggggtctcc ttatgccagg
181gaccaggtta ccctaacagc aggaaggttg tcattaacaa cagtgtcttc agtgttcgct
241ttttcagaac tacagctgta tgcaaggatg acttggttac agtcaaaacc ccagcgtttg
301cagaatctgt cacagaggga gatgtcaggt gggagaaagc tgttggagac acagttgcag
361aagatgaagt ggtttgtgag attgaaactg acaagacatc tgtgcaggtt ccatcaccag
421caaatggcgt gattgaagct cttttggtac ctgatggggg aaaagtcgaa ggaggcactc
481cacttttcac actcaggaaa actggtgctg ctcctgctaa ggccaagccg gctgaagctc
541ctgctgctgc agccccaaaa gcagaaccta cagcagcggc agttcctccc cctgcagcac
601ccatacccac tcagatgcca ccggtgccct cgccctcaca acctccttct ggcaaacctg
661tgtctgcagt aaaacccact gttgccccac cactagctga gccaggagct ggcaaaggtc
721tgcgttcaga acatcgggag aaaatgaaca ggatgcggca gcgcattgct cagcgtctga
781aggaggccca gaatacatgt gcaatgctga caacttttaa tgagattgac atgagtaaca
841tccaggagat gagggctcgg cacaaagagg cttttttgaa gaaacataac ctcaaactag
901gcttcatgtc ggcatttgtg aaggcctcag cctttgcctt gcaggaacag cctgttgtaa
961atgcagtgat tgacgacaca accaaagagg tggtgtatag ggattatatt gacatcagtg
1021ttgcagtggc caccccacgg ggtctggtgg ttccagtcat caggaatgtg gaagctatga
1081attttgcaga tattgaacgg accatcactg aactgggaga gaaggcccga aagaatgaac
1141ttgccattga agatatggat ggcggtacct tcaccattag caatggaggc gtttttggct
1201cgctctttgg aacacccatt atcaaccccc ctcagtctgc catcctgggg atgcatggca
1261tctttgacag gccagtggct ataggaggca aggtagaggt gcggcccatg atgtacgtgg
1321cactgaccta tgatcaccgg ctgattgatg gcagagaggc tgtgactttc ctccgcaaaa
1381tcaaggcagc ggtagaggat cccagagtcc tcctcctgga tctttaccca actttcttgt
1441acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
1501gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)