Transcript: Human NR_033814.2

Homo sapiens dihydrolipoamide S-succinyltransferase (DLST), transcript variant 3, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
DLST (1743)
Length:
2755
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_033814.2
NBCI Gene record:
DLST (1743)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_033814.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000035427 CGCTCTTTGGAACACCCATTA pLKO.1 1116 3UTR 100% 10.800 7.560 N DLST n/a
2 TRCN0000311713 CGCTCTTTGGAACACCCATTA pLKO_005 1116 3UTR 100% 10.800 7.560 N DLST n/a
3 TRCN0000035426 CGTTCAGAACATCGGGAGAAA pLKO.1 638 3UTR 100% 4.950 3.465 N DLST n/a
4 TRCN0000311637 CGTTCAGAACATCGGGAGAAA pLKO_005 638 3UTR 100% 4.950 3.465 N DLST n/a
5 TRCN0000035428 CCAGCAAATGGCGTGATTGAA pLKO.1 332 3UTR 100% 5.625 3.375 N DLST n/a
6 TRCN0000349397 CCAGCAAATGGCGTGATTGAA pLKO_005 332 3UTR 100% 5.625 3.375 N DLST n/a
7 TRCN0000035424 GCCTGTTGTAAATGCAGTGAT pLKO.1 865 3UTR 100% 4.950 2.970 N DLST n/a
8 TRCN0000311638 GCCTGTTGTAAATGCAGTGAT pLKO_005 865 3UTR 100% 4.950 2.970 N DLST n/a
9 TRCN0000035425 CGAAAGAATGAACTTGCCATT pLKO.1 1043 3UTR 100% 4.050 2.430 N DLST n/a
10 TRCN0000311639 CGAAAGAATGAACTTGCCATT pLKO_005 1043 3UTR 100% 4.050 2.430 N DLST n/a
11 TRCN0000344818 CCCTAGTGCTGGTATACTATA pLKO_005 1657 3UTR 100% 13.200 6.600 Y DLST n/a
12 TRCN0000344755 CTAGGCTTCATGTCGGCATTT pLKO_005 812 3UTR 100% 10.800 5.400 Y DLST n/a
13 TRCN0000177977 GCAGATATTGAACGGACCATT pLKO.1 1001 3UTR 100% 4.950 2.970 N Dlst n/a
14 TRCN0000340634 GCAGATATTGAACGGACCATT pLKO_005 1001 3UTR 100% 4.950 2.970 N Dlst n/a
15 TRCN0000177249 GCCTTAATGGATGCTCATTAA pLKO.1 1587 3UTR 100% 1.320 0.660 Y Dlst n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_033814.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06100 pDONR223 100% 46.2% None (many diffs) n/a
2 ccsbBroad304_06100 pLX_304 0% 46.2% V5 (many diffs) n/a
3 TRCN0000465484 CGGAAGATATTGTACATCACAGAT pLX_317 28.2% 46.2% V5 (many diffs) n/a
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