Construct: ORF ccsbBroad304_06103
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007459.1_s304c1, BRDN0000409605
- Derived from:
- ccsbBroadEn_06103
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DMD (1756)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06103
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1756 | DMD | dystrophin | NM_004016.3 | 99.9% | 100% | 768C>T |
| 2 | human | 1756 | DMD | dystrophin | NM_004018.3 | 97.9% | 97.9% | 768C>T;1018_1019ins39 |
| 3 | human | 1756 | DMD | dystrophin | NM_004015.3 | 96.4% | 95.4% | (many diffs) |
| 4 | human | 1756 | DMD | dystrophin | NM_004017.3 | 94.3% | 93.3% | (many diffs) |
| 5 | human | 1756 | DMD | dystrophin | NM_004014.2 | 62.6% | 61.6% | (many diffs) |
| 6 | human | 1756 | DMD | dystrophin | NM_004019.3 | 53.4% | 53.5% | 768C>T;1020_1020delGins886 |
| 7 | human | 1756 | DMD | dystrophin | NM_004021.3 | 50.8% | 50.6% | (many diffs) |
| 8 | human | 1756 | DMD | dystrophin | NM_004022.2 | 49.8% | 49.5% | (many diffs) |
| 9 | human | 1756 | DMD | dystrophin | NM_004013.2 | 49.1% | 48.2% | (many diffs) |
| 10 | human | 1756 | DMD | dystrophin | NM_004023.3 | 42% | 41.7% | (many diffs) |
| 11 | human | 1756 | DMD | dystrophin | NM_004020.3 | 40.2% | 39.4% | (many diffs) |
| 12 | human | 1756 | DMD | dystrophin | XM_017029331.1 | 35.9% | 35.7% | (many diffs) |
| 13 | human | 1756 | DMD | dystrophin | NM_004012.4 | 25.8% | 25.4% | (many diffs) |
| 14 | human | 1756 | DMD | dystrophin | NM_004011.4 | 25.8% | 25.4% | (many diffs) |
| 15 | human | 1756 | DMD | dystrophin | XM_006724473.2 | 17.3% | 17.1% | (many diffs) |
| 16 | human | 1756 | DMD | dystrophin | XM_011545467.1 | 17.2% | 17.1% | (many diffs) |
| 17 | human | 1756 | DMD | dystrophin | XM_006724469.3 | 17.1% | 17% | (many diffs) |
| 18 | human | 1756 | DMD | dystrophin | XM_006724468.2 | 17% | 16.9% | (many diffs) |
| 19 | human | 1756 | DMD | dystrophin | NM_004010.3 | 17% | 16.7% | (many diffs) |
| 20 | human | 1756 | DMD | dystrophin | XM_006724470.3 | 16.7% | 16.6% | (many diffs) |
| 21 | human | 1756 | DMD | dystrophin | NM_000109.4 | 16.5% | 16.2% | (many diffs) |
| 22 | human | 1756 | DMD | dystrophin | NM_004009.3 | 16.5% | 16.2% | (many diffs) |
| 23 | human | 1756 | DMD | dystrophin | NM_004006.2 | 16.5% | 16.2% | (many diffs) |
| 24 | human | 1756 | DMD | dystrophin | XM_017029328.1 | 16.1% | 15.8% | (many diffs) |
| 25 | human | 1756 | DMD | dystrophin | XM_006724474.3 | 14.1% | 14% | (many diffs) |
| 26 | human | 1756 | DMD | dystrophin | XM_006724475.2 | 13.5% | 13.2% | (many diffs) |
| 27 | human | 1756 | DMD | dystrophin | XM_011545468.2 | 5.9% | 5.4% | (many diffs) |
| 28 | mouse | 13405 | Dmd | dystrophin, muscular dystrophy | NM_001314034.1 | 94.4% | 99.6% | (many diffs) |
| 29 | mouse | 13405 | Dmd | dystrophin, muscular dystrophy | NM_001314035.1 | 92.3% | 97.6% | (many diffs) |
| 30 | mouse | 13405 | Dmd | dystrophin, muscular dystrophy | NM_001314036.1 | 90.7% | 95.1% | (many diffs) |
| 31 | mouse | 13405 | Dmd | dystrophin, muscular dystrophy | NM_001314037.1 | 88.7% | 93% | (many diffs) |
| 32 | mouse | 13405 | Dmd | dystrophin, muscular dystrophy | XM_006527773.1 | 58.1% | 60.6% | (many diffs) |
| 33 | mouse | 13405 | Dmd | dystrophin, muscular dystrophy | NM_001314038.1 | 49.9% | 53.3% | (many diffs) |
| 34 | mouse | 13405 | Dmd | dystrophin, muscular dystrophy | XM_017318375.1 | 16.3% | 17.1% | (many diffs) |
| 35 | mouse | 13405 | Dmd | dystrophin, muscular dystrophy | XM_006527768.3 | 16.2% | 17% | (many diffs) |
| 36 | mouse | 13405 | Dmd | dystrophin, muscular dystrophy | XM_006527767.3 | 16.1% | 16.9% | (many diffs) |
| 37 | mouse | 13405 | Dmd | dystrophin, muscular dystrophy | XM_017318373.1 | 15.8% | 16.6% | (many diffs) |
| 38 | mouse | 13405 | Dmd | dystrophin, muscular dystrophy | NM_007868.6 | 15.5% | 16.2% | (many diffs) |
| 39 | mouse | 13405 | Dmd | dystrophin, muscular dystrophy | XM_017318374.1 | 15.2% | 15.8% | (many diffs) |
| 40 | mouse | 13405 | Dmd | dystrophin, muscular dystrophy | XM_017318376.1 | 13.2% | 14% | (many diffs) |
| 41 | mouse | 13405 | Dmd | dystrophin, muscular dystrophy | XM_017318377.1 | 12.6% | 13.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1971
- ORF length:
- 1905
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag ggaacagctc aaaggccacg agactcaaac aacttgctgg gaccatccca 121 aaatgacaga gctctaccag tctttagctg acctgaataa tgtcagattc tcagcttata 181 ggactgccat gaaactccga agactgcaga aggccctttg cttggatctc ttgagcctgt 241 cagctgcatg tgatgccttg gaccagcaca acctcaagca aaatgaccag cccatggata 301 tcctgcagat tattaattgt ttgaccacta tttatgaccg cctggagcaa gagcacaaca 361 atttggtcaa cgtccctctc tgcgtggata tgtgtctgaa ctggctgctg aatgtttatg 421 atacgggacg aacagggagg atccgtgtcc tgtcttttaa aactggcatc atttccctgt 481 gtaaagcaca tttggaagac aagtacagat accttttcaa gcaagtggca agttcaacag 541 gattttgtga ccagcgcagg ctgggcctcc ttctgcatga ttctatccaa attccaagac 601 agttgggtga agttgcatcc tttgggggca gtaacattga gccaagtgtc cggagctgct 661 tccaatttgc taataataag ccagagatcg aagcggccct cttcctagac tggatgagac 721 tggaacccca gtccatggtg tggctgcccg tcctgcacag agtggctgct gcagaaactg 781 ccaagcatca ggccaaatgt aacatctgca aagagtgtcc aatcattgga tttaggtaca 841 ggagtctaaa gcactttaat tatgacatct gccaaagctg ctttttttct ggtcgagttg 901 caaaaggcca taaaatgcac tatcccatgg tggaatattg cactccgact acatcaggag 961 aagatgttcg agactttgcc aaggtactaa aaaacaaatt tcgaaccaaa aggtattttg 1021 cgaagcatcc ccgaatgggc tacctgccag tgcagactgt cttagagggg gacaacatgg 1081 aaactcccgt tactctgatc aacttctggc cagtagattc tgcgcctgcc tcgtcccctc 1141 agctttcaca cgatgatact cattcacgca ttgaacatta tgctagcagg ctagcagaaa 1201 tggaaaacag caatggatct tatctaaatg atagcatctc tcctaatgag agcatagatg 1261 atgaacattt gttaatccag cattactgcc aaagtttgaa ccaggactcc cccctgagcc 1321 agcctcgtag tcctgcccag atcttgattt ccttagagag tgaggaaaga ggggagctag 1381 agagaatcct agcagatctt gaggaagaaa acaggaatct gcaagcagaa tatgaccgtc 1441 taaagcagca gcacgaacat aaaggcctgt ccccactgcc gtcccctcct gaaatgatgc 1501 ccacctctcc ccagagtccc cgggatgctg agctcattgc tgaggccaag ctactgcgtc 1561 aacacaaagg ccgcctggaa gccaggatgc aaatcctgga agaccacaat aaacagctgg 1621 agtcacagtt acacaggcta aggcagctgc tggagcaacc ccaggcagag gccaaagtga 1681 atggcacaac ggtgtcctct ccttctacct ctctacagag gtccgacagc agtcagccta 1741 tgctgctccg agtggttggc agtcaaactt cggactccat gggtgaggaa gatcttctca 1801 gtcctcccca ggacacaagc acagggttag aggaggtgat ggagcaactc aacaactcct 1861 tccctagttc aagaggacac aatgtaggaa gtcttttcca catggcagat gatttgggca 1921 gagcgatgga gtccttagta tcagtcatga cagatgaaga aggagcagaa tacccaactt 1981 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 2041 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta