Construct: ORF ccsbBroad304_06106

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000741.1_s304c1, BRDN0000462206
Derived from:
ccsbBroadEn_06106
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DNM1 (1759)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06106
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1759 DNM1 dynamin 1 NM_001005336.3 99.9% 99.8% 1008C>T;1666G>T
2 human 1759 DNM1 dynamin 1 NM_001288737.2 98.4% 98.1% (many diffs)
3 human 1759 DNM1 dynamin 1 NM_001288738.2 98.4% 98.1% (many diffs)
4 human 1759 DNM1 dynamin 1 NM_004408.4 97.9% 97.8% (many diffs)
5 human 1759 DNM1 dynamin 1 NM_001374269.1 97.9% 97.4% (many diffs)
6 human 1759 DNM1 dynamin 1 NM_001288739.2 96.5% 96% (many diffs)
7 mouse 13429 Dnm1 dynamin 1 XM_006497661.2 92.5% 99.5% (many diffs)
8 mouse 13429 Dnm1 dynamin 1 XM_017315328.1 92.5% 99.5% (many diffs)
9 mouse 13429 Dnm1 dynamin 1 NM_001301737.1 91.2% 97.8% (many diffs)
10 mouse 13429 Dnm1 dynamin 1 XM_006497658.3 91.2% 97.8% (many diffs)
11 mouse 13429 Dnm1 dynamin 1 XM_006497659.3 91.2% 97.8% (many diffs)
12 mouse 13429 Dnm1 dynamin 1 XM_006497660.2 91.2% 97.8% (many diffs)
13 mouse 13429 Dnm1 dynamin 1 XM_006497655.2 90.7% 97.4% (many diffs)
14 mouse 13429 Dnm1 dynamin 1 XM_006497650.2 90.6% 97% (many diffs)
15 mouse 13429 Dnm1 dynamin 1 NM_010065.3 90.1% 96.5% (many diffs)
16 mouse 13429 Dnm1 dynamin 1 XM_006497654.3 89.4% 95.8% (many diffs)
17 mouse 13429 Dnm1 dynamin 1 XM_006497651.2 89.3% 95.5% (many diffs)
18 mouse 13429 Dnm1 dynamin 1 XM_006497649.2 89.2% 95.3% (many diffs)
19 mouse 13429 Dnm1 dynamin 1 XM_006497652.2 89.2% 95.3% (many diffs)
20 mouse 13429 Dnm1 dynamin 1 XM_006497653.2 88.8% 94.9% (many diffs)
21 mouse 13429 Dnm1 dynamin 1 XR_374057.2 83.5% (many diffs)
22 mouse 13429 Dnm1 dynamin 1 XM_006497662.2 82.8% 86.5% (many diffs)
23 mouse 13429 Dnm1 dynamin 1 XR_374058.3 70.4% (many diffs)
24 mouse 13429 Dnm1 dynamin 1 XR_001780678.1 69.9% (many diffs)
25 mouse 13429 Dnm1 dynamin 1 NR_125959.1 69.4% (many diffs)
26 mouse 13429 Dnm1 dynamin 1 XR_374055.2 60% (many diffs)
27 mouse 13429 Dnm1 dynamin 1 XR_001780675.1 59.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2622
ORF length:
2553
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggcaaccgc ggcatggaag atctcatccc gctggtcaac cggctgcaag
121acgccttctc tgccatcggc cagaacgcgg acctcgacct gccgcagatc gctgtggtgg
181gcggccagag cgccggcaag agctcggtgc tcgagaattt cgtaggcagg gacttcttgc
241ctcgaggatc tggcattgtc acccgacgtc ccctggtctt gcagctggtc aatgcaacca
301cagaatatgc cgagttcctg cactgcaagg gaaagaaatt caccgacttc gaggaggtgc
361gccttgagat cgaggccgag accgacaggg tcaccggcac caacaagggc atctcgccgg
421tgcctatcaa cctccgcgtc tactcgccgc acgtgctgaa cctgaccctg gtggacctgc
481ccggaatgac caaggtcccg gtgggggacc aacctcccga catcgagttc cagatccgag
541acatgcttat gcagtttgtc accaaggaga actgcctcat cctggccgtg tcccccgcca
601actctgacct ggccaattct gacgccctca aggtcgccaa ggaggtggac ccccagggcc
661agcgcaccat cggggtcatc accaagctgg acctgatgga cgagggcaca gatgcccgtg
721atgtgctgga gaacaagctg ctccccctgc gcagaggcta cattggagtg gtgaaccgga
781gccagaagga cattgatggc aagaaggaca ttaccgccgc cttggctgct gaacgaaagt
841tcttcctctc ccatccatct tatcgccact tggctgaccg tatgggcacg ccctacctgc
901agaaggtcct caatcagcaa ctgacgaacc acatccggga cacactgccg gggctgcgga
961acaagctgca gagccagcta ctgtccattg agaaggaggt ggaggaatac aagaacttcc
1021gccctgatga cccagctcgc aagaccaagg ccctgctgca gatggtccag cagtttgccg
1081tagactttga gaagcgcatt gagggctcag gagatcagat cgacacctac gaactgtcag
1141ggggagcccg cattaaccga atcttccacg agcgcttccc tttcgagctg gtcaagatgg
1201agtttgatga gaaggaactc cgaagggaga tcagctatgc tatcaagaat atccatggca
1261ttagaacggg gctgtttacc ccagacatgg cctttgagac cattgtgaaa aagcaggtga
1321agaagatccg agaaccgtgt ctcaagtgtg tggacatggt tatctcggag ctaatcagca
1381ccgttagaca gtgcaccaag aagctccagc agtacccgcg gctacgggag gagatggagc
1441gcatcgtgac cacccacatc cgggagcgcg agggccgcac taaggagcag gtcatgcttc
1501tcatcgatat cgagctggct tacatgaaca ccaaccatga ggacttcata ggctttgcca
1561atgctcagca gaggagcaac cagatgaaca agaagaagac ttcagggaac caggatgaga
1621ttctggtcat ccgcaagggc tggctgacta tcaataatat tggcatcatg aaagggggct
1681ccaaggagta ctggtttgtg ctgactgctg agaatctgtc ctggtacaag gattatgagg
1741agaaagagaa gaaatacatg ctgtctgtgg acaacctcaa gctgcgggac gtggagaagg
1801gctttatgtc gagcaagcat atctttgccc tctttaacac ggagcagagg aatgtctaca
1861aggattatcg gcagctggag ctagcctgtg agacacagga ggaggtggac agctggaagg
1921cctccttcct gagggctggc gtgtaccctg agcgtgttgg ggacaaagag aaagccagcg
1981agaccgagga gaatggctcc gacagcttca tgcattccat ggacccacag ctggaacggc
2041aagtggagac catccggaat cttgtggact catacatggc cattgtcaac aagaccgtga
2101gggacctcat gcccaagacc atcatgcacc tcatgattaa caataccaag gagttcatct
2161tctcggagct gctggccaac ctgtactcgt gtggggacca gaacacgctg atggaggagt
2221cggcggagca ggcacagcgg cgcgacgaga tgctgcgcat gtaccacgca ctgaaggagg
2281cgctcagcat catcggcgac atcaacacga ccaccgtcag cacgcccatg cccccgcccg
2341tggacgactc ctggctgcag gtgcagagcg taccggccgg acgcaggtcg cccacgtcca
2401gccccacgcc gcagcgccga gcccccgccg tgcccccagc ccggcccggg tcgcggggcc
2461ctgctcctgg gcctccgcct gctgggtccg ccctgggggg ggcgcccccc gtgccctcca
2521ggccgggggc ttcccctgac cctttcggcc ctccccctca ggtgccctcg cgccccaacc
2581gcgccccgcc cggggtcccc agaatcacta tcagtgaccc cttgccaact ttcttgtaca
2641aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
2701ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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