Construct: ORF ccsbBroad304_06177

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003005.1_s304c1, BRDN0000404363
Derived from:
ccsbBroadEn_06177
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EYA4 (2070)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06177
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2070 EYA4 EYA transcriptional coactiv... NM_172103.3 99.9% 100% 714G>A
2 human 2070 EYA4 EYA transcriptional coactiv... NM_001370458.1 98.2% 98.2% (many diffs)
3 human 2070 EYA4 EYA transcriptional coactiv... NM_004100.5 96.3% 96.2% 209_277del;783G>A
4 human 2070 EYA4 EYA transcriptional coactiv... XM_017010370.2 95.6% 92.9% (many diffs)
5 human 2070 EYA4 EYA transcriptional coactiv... NM_001301013.1 95.4% 95.3% 209_277del;783G>A;967_984del
6 human 2070 EYA4 EYA transcriptional coactiv... XM_017010371.2 94.9% 92.1% (many diffs)
7 human 2070 EYA4 EYA transcriptional coactiv... NM_172105.3 94.7% 94.5% (many diffs)
8 human 2070 EYA4 EYA transcriptional coactiv... NM_001370459.1 93.9% 94% 206_207ins93;621G>A;805_822del
9 human 2070 EYA4 EYA transcriptional coactiv... XM_005266851.5 93.8% 93.6% (many diffs)
10 human 2070 EYA4 EYA transcriptional coactiv... NM_001301012.1 93.2% 93.1% (many diffs)
11 human 2070 EYA4 EYA transcriptional coactiv... XM_017010369.2 93.1% 90.4% (many diffs)
12 human 2070 EYA4 EYA transcriptional coactiv... XM_017010368.2 92.3% 89.5% (many diffs)
13 human 2070 EYA4 EYA transcriptional coactiv... XM_017010374.2 92.3% 92.2% (many diffs)
14 human 2070 EYA4 EYA transcriptional coactiv... XM_017010373.2 91.6% 88.9% (many diffs)
15 human 2070 EYA4 EYA transcriptional coactiv... XM_017010372.2 90.8% 88.1% (many diffs)
16 human 2070 EYA4 EYA transcriptional coactiv... XR_001743219.2 68.9% (many diffs)
17 human 2070 EYA4 EYA transcriptional coactiv... XR_001743220.2 19.4% (many diffs)
18 mouse 14051 Eya4 EYA transcriptional coactiv... NM_010167.5 90.6% 96.5% (many diffs)
19 mouse 14051 Eya4 EYA transcriptional coactiv... NM_001347372.1 89% 94.8% (many diffs)
20 mouse 14051 Eya4 EYA transcriptional coactiv... XM_006512523.3 89% 94.8% (many diffs)
21 mouse 14051 Eya4 EYA transcriptional coactiv... XM_006512524.3 89% 94.8% (many diffs)
22 mouse 14051 Eya4 EYA transcriptional coactiv... XM_017313799.1 87.4% 92.9% (many diffs)
23 mouse 14051 Eya4 EYA transcriptional coactiv... XM_011243112.2 87% 91.1% (many diffs)
24 mouse 14051 Eya4 EYA transcriptional coactiv... XM_017313798.1 87% 91.1% (many diffs)
25 mouse 14051 Eya4 EYA transcriptional coactiv... XM_006512526.3 86.8% 90.4% (many diffs)
26 mouse 14051 Eya4 EYA transcriptional coactiv... XM_006512527.3 86.5% 91.4% (many diffs)
27 mouse 14051 Eya4 EYA transcriptional coactiv... XM_006512528.3 84.9% 90.9% (many diffs)
28 mouse 14051 Eya4 EYA transcriptional coactiv... XM_011243115.1 84.9% 90.9% (many diffs)
29 mouse 14051 Eya4 EYA transcriptional coactiv... XM_006512529.3 84.3% 90.2% (many diffs)
30 mouse 14051 Eya4 EYA transcriptional coactiv... XM_006512530.3 69.3% 74.8% (many diffs)
31 mouse 14051 Eya4 EYA transcriptional coactiv... XR_001779457.1 54.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1914
ORF length:
1848
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga agactcccag gatttaaatg aacaatcagt aaagaaaacg tgcacagaat
121cagatgtttc acaatctcag aattccaggt ctatggaaat gcaggaccta gcaagtcctc
181atactcttgt tggaggtggt gatactccag gtagctccaa actggaaaaa tctaatctca
241gcagcacatc agttactaca aatgggacag gagtgtctct tcttgcagtc aaaacagagc
301ccttgaacag cagtgaaacc acagccacga ctggagatgg agcgcttgac acttttactg
361ggtcagtaat tacaagtagt ggctacagcc ccagatcagc acatcagtat tccccacagc
421tgtatccttc caagccctat ccacacattc tttctacacc agcagctcaa acaatgtctg
481cctatgcagg ccagactcag tattcgggga tgcagcagcc agccgtctac acagcctact
541cacagacagg acagccctac agcttgccca cttacgattt gggtgtgatg ttgccagcca
601tcaagacaga gagtggactt tcccaaactc agtccccatt acagagtggc tgcctcagtt
661acagcccagg gttctctacc ccacagccag gccagacacc ttattcttac caaatgccag
721gttctagttt tgcaccatca tctactattt atgcaaataa ttcagtttcc aattcaacaa
781atttcagtgg ttcacaacag gattatccat cctatacagc ctttggccaa aaccagtatg
841cacagtatta ttcagcatca acgtatggag cgtatatgac atcgaataac acagccgatg
901gcacaccctc ttcaacctct acttatcagt tgcaggaatc tctcccagga ctgactaacc
961aaccaggaga gttcgatacc atgcagagtc cctccacacc catcaaagat cttgatgaga
1021gaacctgtag gagttctggg tcaaagtcca gaggaagagg ccggaaaaat aatccctccc
1081cgcctcctga tagtgacctg gagcgtgtgt ttgtctggga tttggatgaa accatcattg
1141tttttcactc actgctcacc gggtcttatg cacagaagta tggcaaggat ccccccatgg
1201ctgtaaccct tggactccgc atggaagaaa tgatttttaa tcttgctgat actcatttgt
1261tttttaatga tttagaggag tgtgatcaag ttcatataga tgatgtttcc tctgatgata
1321atgggcagga cttaagtacc tacagttttg caactgatgg cttccatgca gctgcaagta
1381gtgcaaacct ttgtttgcca acaggtgtaa gaggaggggt tgactggatg aggaagttgg
1441cttttcgtta cagaagagta aaagaattat ataacaccta caagaacaac gttggaggac
1501tccttggccc tgccaagagg gatgcctggc tacagttaag ggcagagatt gaaggtctga
1561cagattcctg gctaacaaat gcacttaagt ctttatcaat tattagcact aggagtaact
1621gcataaatgt cttggtaacg acaactcaac tgatcccagc acttgcgaag gttctactct
1681atagtttagg aggtgctttc cccattgaga atatttacag tgcaactaaa ataggaaaag
1741aaagttgctt tgaacgaata atgcaaaggt ttggcagaaa agtagtgtat gttgtaattg
1801gggatggtgt agaagaagaa caggcagcaa aaaagcacaa catgcccttc tggaggatat
1861ccagtcactc agacctcctg gctctccacc aagcactgga attagagtat ttgtacccaa
1921ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
1981cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

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