Construct: ORF ccsbBroadEn_06177
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003005.1_s300c1, BRDN0000390430
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EYA4 (2070)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2070 | EYA4 | EYA transcriptional coactiv... | NM_172103.3 | 99.9% | 100% | 714G>A |
| 2 | human | 2070 | EYA4 | EYA transcriptional coactiv... | NM_001370458.1 | 98.2% | 98.2% | (many diffs) |
| 3 | human | 2070 | EYA4 | EYA transcriptional coactiv... | NM_004100.5 | 96.3% | 96.2% | 209_277del;783G>A |
| 4 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_017010370.2 | 95.6% | 92.9% | (many diffs) |
| 5 | human | 2070 | EYA4 | EYA transcriptional coactiv... | NM_001301013.1 | 95.4% | 95.3% | 209_277del;783G>A;967_984del |
| 6 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_017010371.2 | 94.9% | 92.1% | (many diffs) |
| 7 | human | 2070 | EYA4 | EYA transcriptional coactiv... | NM_172105.3 | 94.7% | 94.5% | (many diffs) |
| 8 | human | 2070 | EYA4 | EYA transcriptional coactiv... | NM_001370459.1 | 93.9% | 94% | 206_207ins93;621G>A;805_822del |
| 9 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_005266851.5 | 93.8% | 93.6% | (many diffs) |
| 10 | human | 2070 | EYA4 | EYA transcriptional coactiv... | NM_001301012.1 | 93.2% | 93.1% | (many diffs) |
| 11 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_017010369.2 | 93.1% | 90.4% | (many diffs) |
| 12 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_017010368.2 | 92.3% | 89.5% | (many diffs) |
| 13 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_017010374.2 | 92.3% | 92.2% | (many diffs) |
| 14 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_017010373.2 | 91.6% | 88.9% | (many diffs) |
| 15 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_017010372.2 | 90.8% | 88.1% | (many diffs) |
| 16 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XR_001743219.2 | 68.9% | (many diffs) | |
| 17 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XR_001743220.2 | 19.4% | (many diffs) | |
| 18 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | NM_010167.5 | 90.6% | 96.5% | (many diffs) |
| 19 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | NM_001347372.1 | 89% | 94.8% | (many diffs) |
| 20 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_006512523.3 | 89% | 94.8% | (many diffs) |
| 21 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_006512524.3 | 89% | 94.8% | (many diffs) |
| 22 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_017313799.1 | 87.4% | 92.9% | (many diffs) |
| 23 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_011243112.2 | 87% | 91.1% | (many diffs) |
| 24 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_017313798.1 | 87% | 91.1% | (many diffs) |
| 25 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_006512526.3 | 86.8% | 90.4% | (many diffs) |
| 26 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_006512527.3 | 86.5% | 91.4% | (many diffs) |
| 27 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_006512528.3 | 84.9% | 90.9% | (many diffs) |
| 28 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_011243115.1 | 84.9% | 90.9% | (many diffs) |
| 29 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_006512529.3 | 84.3% | 90.2% | (many diffs) |
| 30 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_006512530.3 | 69.3% | 74.8% | (many diffs) |
| 31 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XR_001779457.1 | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1914
- ORF length:
- 1848
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA AGACTCCCAG GATTTAAATG AACAATCAGT AAAGAAAACG TGCACAGAAT 121 CAGATGTTTC ACAATCTCAG AATTCCAGGT CTATGGAAAT GCAGGACCTA GCAAGTCCTC 181 ATACTCTTGT TGGAGGTGGT GATACTCCAG GTAGCTCCAA ACTGGAAAAA TCTAATCTCA 241 GCAGCACATC AGTTACTACA AATGGGACAG GAGTGTCTCT TCTTGCAGTC AAAACAGAGC 301 CCTTGAACAG CAGTGAAACC ACAGCCACGA CTGGAGATGG AGCGCTTGAC ACTTTTACTG 361 GGTCAGTAAT TACAAGTAGT GGCTACAGCC CCAGATCAGC ACATCAGTAT TCCCCACAGC 421 TGTATCCTTC CAAGCCCTAT CCACACATTC TTTCTACACC AGCAGCTCAA ACAATGTCTG 481 CCTATGCAGG CCAGACTCAG TATTCGGGGA TGCAGCAGCC AGCCGTCTAC ACAGCCTACT 541 CACAGACAGG ACAGCCCTAC AGCTTGCCCA CTTACGATTT GGGTGTGATG TTGCCAGCCA 601 TCAAGACAGA GAGTGGACTT TCCCAAACTC AGTCCCCATT ACAGAGTGGC TGCCTCAGTT 661 ACAGCCCAGG GTTCTCTACC CCACAGCCAG GCCAGACACC TTATTCTTAC CAAATGCCAG 721 GTTCTAGTTT TGCACCATCA TCTACTATTT ATGCAAATAA TTCAGTTTCC AATTCAACAA 781 ATTTCAGTGG TTCACAACAG GATTATCCAT CCTATACAGC CTTTGGCCAA AACCAGTATG 841 CACAGTATTA TTCAGCATCA ACGTATGGAG CGTATATGAC ATCGAATAAC ACAGCCGATG 901 GCACACCCTC TTCAACCTCT ACTTATCAGT TGCAGGAATC TCTCCCAGGA CTGACTAACC 961 AACCAGGAGA GTTCGATACC ATGCAGAGTC CCTCCACACC CATCAAAGAT CTTGATGAGA 1021 GAACCTGTAG GAGTTCTGGG TCAAAGTCCA GAGGAAGAGG CCGGAAAAAT AATCCCTCCC 1081 CGCCTCCTGA TAGTGACCTG GAGCGTGTGT TTGTCTGGGA TTTGGATGAA ACCATCATTG 1141 TTTTTCACTC ACTGCTCACC GGGTCTTATG CACAGAAGTA TGGCAAGGAT CCCCCCATGG 1201 CTGTAACCCT TGGACTCCGC ATGGAAGAAA TGATTTTTAA TCTTGCTGAT ACTCATTTGT 1261 TTTTTAATGA TTTAGAGGAG TGTGATCAAG TTCATATAGA TGATGTTTCC TCTGATGATA 1321 ATGGGCAGGA CTTAAGTACC TACAGTTTTG CAACTGATGG CTTCCATGCA GCTGCAAGTA 1381 GTGCAAACCT TTGTTTGCCA ACAGGTGTAA GAGGAGGGGT TGACTGGATG AGGAAGTTGG 1441 CTTTTCGTTA CAGAAGAGTA AAAGAATTAT ATAACACCTA CAAGAACAAC GTTGGAGGAC 1501 TCCTTGGCCC TGCCAAGAGG GATGCCTGGC TACAGTTAAG GGCAGAGATT GAAGGTCTGA 1561 CAGATTCCTG GCTAACAAAT GCACTTAAGT CTTTATCAAT TATTAGCACT AGGAGTAACT 1621 GCATAAATGT CTTGGTAACG ACAACTCAAC TGATCCCAGC ACTTGCGAAG GTTCTACTCT 1681 ATAGTTTAGG AGGTGCTTTC CCCATTGAGA ATATTTACAG TGCAACTAAA ATAGGAAAAG 1741 AAAGTTGCTT TGAACGAATA ATGCAAAGGT TTGGCAGAAA AGTAGTGTAT GTTGTAATTG 1801 GGGATGGTGT AGAAGAAGAA CAGGCAGCAA AAAAGCACAA CATGCCCTTC TGGAGGATAT 1861 CCAGTCACTC AGACCTCCTG GCTCTCCACC AAGCACTGGA ATTAGAGTAT TTGTACCCAA 1921 CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac