Construct: ORF ccsbBroad304_06179
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014130.1_s304c1, BRDN0000407682
- Derived from:
- ccsbBroadEn_06179
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ERG (2078)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06179
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2078 | ERG | ETS transcription factor ERG | NM_182918.4 | 88.9% | 73.6% | (many diffs) |
| 2 | human | 2078 | ERG | ETS transcription factor ERG | NM_001136154.1 | 87.5% | 72% | (many diffs) |
| 3 | human | 2078 | ERG | ETS transcription factor ERG | NM_001243428.1 | 87.5% | 72% | (many diffs) |
| 4 | human | 2078 | ERG | ETS transcription factor ERG | NM_001331025.2 | 84% | 68.6% | (many diffs) |
| 5 | human | 2078 | ERG | ETS transcription factor ERG | NM_004449.4 | 82.7% | 67% | (many diffs) |
| 6 | human | 2078 | ERG | ETS transcription factor ERG | NM_001136155.1 | 70.1% | 54.4% | (many diffs) |
| 7 | human | 2078 | ERG | ETS transcription factor ERG | NM_001243429.1 | 65.2% | 49.4% | (many diffs) |
| 8 | human | 2078 | ERG | ETS transcription factor ERG | NM_001243432.2 | 63.6% | 84.2% | (many diffs) |
| 9 | human | 2078 | ERG | ETS transcription factor ERG | NM_001291391.1 | 58.9% | 68.5% | (many diffs) |
| 10 | mouse | 13876 | Erg | ETS transcription factor | NM_001302153.1 | 81% | 71.3% | (many diffs) |
| 11 | mouse | 13876 | Erg | ETS transcription factor | NM_133659.3 | 79.8% | 69.7% | (many diffs) |
| 12 | mouse | 13876 | Erg | ETS transcription factor | XM_006522894.3 | 79.8% | 69.7% | (many diffs) |
| 13 | mouse | 13876 | Erg | ETS transcription factor | XM_006522895.2 | 79.8% | 69.7% | (many diffs) |
| 14 | mouse | 13876 | Erg | ETS transcription factor | XM_006522896.3 | 79.8% | 69.7% | (many diffs) |
| 15 | mouse | 13876 | Erg | ETS transcription factor | NM_001302154.1 | 76.9% | 67% | (many diffs) |
| 16 | mouse | 13876 | Erg | ETS transcription factor | NM_001302179.1 | 75.7% | 65.4% | (many diffs) |
| 17 | mouse | 13876 | Erg | ETS transcription factor | XM_006522899.3 | 75.7% | 65.4% | (many diffs) |
| 18 | mouse | 13876 | Erg | ETS transcription factor | NM_001302152.1 | 75% | 64.8% | (many diffs) |
| 19 | mouse | 13876 | Erg | ETS transcription factor | XM_006522900.2 | 75% | 64.8% | (many diffs) |
| 20 | mouse | 13876 | Erg | ETS transcription factor | XM_006522904.3 | 75% | 64.7% | (many diffs) |
| 21 | mouse | 13876 | Erg | ETS transcription factor | NM_001302183.1 | 71% | 60.4% | (many diffs) |
| 22 | mouse | 13876 | Erg | ETS transcription factor | XM_006522903.3 | 71% | 60.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1131
- ORF length:
- 1065
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cagcactatt aaggaagcct tatcagttgt gagtgaggac cagtcgttgt 121 ttgagtgtgc ctacggaacg ccacacctgg ctaagacaga gatgaccgcg tcctcctcca 181 gcgactatgg acagacttcc aagatgagcc cacgcgtccc tcagcaggat tggctgtctc 241 aacccccagc cagggtcacc atcaaaatgg aatgtaaccc tagccaggtg aatggctcaa 301 ggaactctcc tgatgaatgc agtgtggcca aaggcgggaa gatggtgggc agcccagaca 361 ccgttgggat gaactacggc agctacatgg aggagaagca catgccaccc ccaaacatga 421 ccacgaacga gcgcagagtt atcgtgccag cagatcctac gctatggagt acagaccatg 481 tgcggcagtg gctggagtgg gcggtgaaag aatatggcct tccagacgtc aacatcttgt 541 tattccagaa catcgatggg aaggaactgt gcaagatgac caaggacgac ttccagaggc 601 tcacccccag ctacaatgcc gacatccttc tctcacatct ccactacctc agagagactc 661 ctcttccaca tttgacttca gatgatgttg ataaagcctt acaaaactct ccacggttaa 721 tgcatgctag aaacacaggg ggtgcagctt ttattttccc aaatacttca gtatatcctg 781 aagctacgca aagaattaca actaggccag atttaccata tgagcccccc aggagatcag 841 cctggaccgg tcacggccac cccacgcccc agtcgaaagc tgctcaacca tctccttcca 901 cagtgcccaa aactgaagac cagcgtcctc agttagatcc ttatcagatt cttggaccaa 961 caagtagccg ccttgcaaat ccaggcagtg gccagatcca gctttggcag ttcctcctgg 1021 agctcctgtc ggacagctcc aactccagct gcatcacctg ggaaggcacc aacggggagt 1081 tcaagatgac ggatcccgac gaggtggccc ggcgctgggg aGAATGGATG TGAACAATCA 1141 TCTTGTACTA CGAAGAGCTC GCCCGCTCCT TCCAAAATAA AAGGACAATA TCATCAGACC 1201 CAGCCCAGGT AAAGGCTAGT CATACAACTG TCACTTCTTC CCAGGGCCAC GGATGGAAAC 1261 CCCACGCCCC GGAGTAAATT CGTACCAGTA CCCGTCAGAC ATACTTATGA AAGCTCGAGT 1321 CCAGGCCAAC CACACAGGAG AGCGCAGTCG GGTCCGCGCC CTCCAGCCCC ACAAGTGACG 1381 TAATGCGGGT GTTCTACTCC CCCAAGACCG TCCTGGAATT CAGCACATAC ATGCAAGAGA 1441 CCAACAAGAG GCTCCCCACC TGCCATCCCC GGACTCGTCT GGGCGTTTAC TACTAACCAT 1501 CTTTCTTAAA CAAAGTGGTA AGTAAGCCTA TCCCCCTCCC TCTCCTCGGT ATTGAATCTA 1561 GGAAGTAATG AGCTAGCGCT AACCGGTGGC GCGTTAAGTC AACAATCATC CTCTGGATAC 1621 AAAATTTGTG AAAGAAGGAC TGTTATTATT AAGTTGGTCC CTCTTTGCCG CTATGTGGAT 1681 ACGCTAATTT AATGCCATTG TATCTAGTTT TTCCCCG