Construct: ORF ccsbBroad304_06219
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002739.1_s304c1, BRDN0000462382
- Derived from:
- ccsbBroadEn_06219
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AFF2 (2334)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06219
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2334 | AFF2 | AF4/FMR2 family member 2 | NM_001169123.2 | 99.9% | 99.8% | 209C>A;3098C>T |
| 2 | human | 2334 | AFF2 | AF4/FMR2 family member 2 | NM_002025.4 | 99.1% | 99% | (many diffs) |
| 3 | human | 2334 | AFF2 | AF4/FMR2 family member 2 | NM_001169125.2 | 97.7% | 97.6% | 209C>A;1073_1074ins87;3011C>T |
| 4 | human | 2334 | AFF2 | AF4/FMR2 family member 2 | NM_001169124.2 | 97.4% | 97.3% | (many diffs) |
| 5 | human | 2334 | AFF2 | AF4/FMR2 family member 2 | NM_001169122.2 | 97.1% | 97% | (many diffs) |
| 6 | human | 2334 | AFF2 | AF4/FMR2 family member 2 | NM_001170628.1 | 71.8% | 71.1% | (many diffs) |
| 7 | mouse | 14266 | Aff2 | AF4/FMR2 family, member 2 | NM_008032.3 | 85.4% | 86% | (many diffs) |
| 8 | mouse | 14266 | Aff2 | AF4/FMR2 family, member 2 | XM_006527821.3 | 85.3% | 85.8% | (many diffs) |
| 9 | mouse | 14266 | Aff2 | AF4/FMR2 family, member 2 | XM_006527820.3 | 85% | 85.6% | (many diffs) |
| 10 | mouse | 14266 | Aff2 | AF4/FMR2 family, member 2 | XM_006527819.3 | 83.6% | 82.7% | (many diffs) |
| 11 | mouse | 14266 | Aff2 | AF4/FMR2 family, member 2 | XM_006527818.3 | 83.4% | 82.5% | (many diffs) |
| 12 | mouse | 14266 | Aff2 | AF4/FMR2 family, member 2 | XM_006527817.3 | 83.2% | 82.4% | (many diffs) |
| 13 | mouse | 14266 | Aff2 | AF4/FMR2 family, member 2 | XM_006527816.3 | 83% | 82.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3972
- ORF length:
- 3903
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatctattc gactttttca gagactggga cttggagcag cagtgtcact 121 atgaacaaga ccgtagtgca cttaaaaaaa gggaatggga gcggaggaat caagaagtcc 181 agcaagaaga cgatctcttt tcttcaggct ttgatctttt tggggagcca tacaagacaa 241 acaaaggtga tgcacttgcc aaccgagtcc agaacaagct tggaaactat gatgaaatga 301 agaatttgct aactaaccat tctaatcaga atcacctagt gggaattcca aagaattctg 361 tgccccagaa tcccaacaac aaaaatgaac caagcttttt tccagaacaa aagaacagaa 421 taattccacc tcaccaggat aatacccatc cttcagcacc aatgcctcca ccttctgttg 481 tgatactgaa ttcaactcta atacacagca acagaaaatc aaaacctgag tggtcacgtg 541 atagtcataa ccctagcact gtactggcaa gccaggccag tggtcagcca aacaagatgc 601 agactttgac acaggaccag tctcaagcca aactggaaga cttctttgtc tacccagctg 661 aacagcccca gattggagaa gttgaagagt caaacccatc tgcaaaggaa gacagtaacc 721 ctaattctag tggagaagat gctttcaaag aaatctttca atccaattca ccggaagaat 781 ctgaattcgc cgtgcaagcg cctgggtctc ccctagtggc ttcctcttta ttagctccta 841 gcagtggcct ttcagttcaa aacttcccac cagggcttta ctgcaaaaca agcatggggc 901 agcaaaagcc aactgcatac gtcagaccca tggatggcca ggaccaggca ccggacatct 961 caccaacact gaaaccttca attgaatttg agaacagctt tgggaatctg tcatttggaa 1021 cactcttgga tggaaaaccc agtgcagcca gttcaaagac taaactgcca aagttcacca 1081 tcctccaaac aagtgaagta agccttccca gtgatccaag ctgtgttgaa gaaatcttgc 1141 gggagatgac ccattcctgg cctactcctc tcacttccat gcatactgct ggacactctg 1201 agcagagcac cttttccatc ccaggacagg aatcgcagca tctgacccca ggattcacct 1261 tacaaaagtg gaatgaccca accaccagag cttctacaaa gatgcttgag gatgacctga 1321 agctgagcag tgatgaagat gaccttgagc ctgtgaagac cttgaccact cagtgcactg 1381 ccactgagct ctaccaggct gttgaaaagg caaaacctag gaataatcct gtgaacccac 1441 ccttggccac tccccagccc ccacctgcag tgcaagccag cgggggttct ggcagctcca 1501 gcgaatcgga gagcagctct gagtcggatt cagacactga aagtagcacc actgacagcg 1561 aatctaatga ggcacctcgt gtggcaactc cagagcctga gccaccctca accaacaagt 1621 ggcaactgga taaatggctt aacaaagtga catcccagaa caagtctttt atttgtggcc 1681 aaaatgaaac acccatggag actatttctc tgcctcctcc aatcatccaa ccaatggaag 1741 tccagatgaa agtgaagacg aatgccagtc aggtcccagc tgaacccaaa gaaaggcctc 1801 tcctcagtct cattagggag aaagcccgtc cacggcccac tcagaaaatt ccagaaacaa 1861 aggctttgaa gcataagttg tcaacaacta gtgagacagt gtctcaaagg acaattggga 1921 aaaaacagcc caaaaaagtt gagaagaaca ccagcactga cgagtttacc tggcccaaac 1981 caaatattac cagcagcact cccaaagaaa aagaaagtgt ggagcttcat gacccaccaa 2041 gaggccgcaa caaagccact gcccacaaac cagcccctag gaaagaacca agacctaaca 2101 tccctttggc tcccgagaag aagaagtaca gagggcctgg caagattgtg ccaaagtctc 2161 gggaattcat tgaaacagat tcatctacat ctgactccaa cacagatcag gaagagaccc 2221 tgcaaatcaa agtcctgcct ccgtgcatta tttctggagg taatactgcc aaatccaagg 2281 aaatctgtgg tgccagcctg accctcagca ccttaatgag tagcagtggc agcaacaaca 2341 acttatccat cagtaatgaa gagccaacat tttcacctat tcctgtcatg caaactgaaa 2401 tcctgtcccc tctgcgagat catgagaacc tgaaaaacct ctgggtgaag attgaccttg 2461 acttactctc tagagtacct ggccacagct cactccatgc agcacctgcc aagccagacc 2521 acaaggagac tgccacaaaa cccaagcgtc agacagctgt cacagctgtg gagaaaccag 2581 cccctaaggg caaacgtaag cacaagccaa tagaagttgc agagaagatc cctgagaaga 2641 agcagcgcct ggaggaggcc acaactatct gcttgctccc tccttgcatc tcaccagccc 2701 caccccacaa gcctcccaac actagagaaa ataattcatc caggagagca aatagaagaa 2761 aggaagaaaa actatttcct cctccacttt ccccactgcc agaggaccct ccacgccgca 2821 gaaatgtcag tggcaataat ggtccctttg gtcaagacaa aaacatcgcc atgactggac 2881 aaatcacatc taccaaacct aagagaactg aaggcaaatt ctgtgctact ttcaaaggga 2941 tatcggtaaa tgagggagac actccaaaaa aggcatcctc tgccaccatc actgtcacca 3001 atactgctat tgccactgct actgtcactg ctactgccat tgtcaccacc actgtcacag 3061 ctactgccac cgccacggcc accaccacaa ctactaccac taccatttcc accatcacct 3121 ctaccatcac tactggcctc atggatagca gtcacctgga gatgatgtcc tgggcggctc 3181 tgccccttct atccagcagc agcactaatg tccggagacc caagctcact tttgatgact 3241 cggttcacaa tgctgattat tacatgcaag aagctaagaa gctgaagcac aaagctgatg 3301 cactgttcga gaaatttggc aaagctgtga attatgctga tgccgccctc tccttcactg 3361 aatgtggcaa tgccatggaa cgcgaccctc tggaagcaaa gtccccatac accatgtact 3421 ctgagactgt ggagctcctc aggtatgcaa tgaggctgaa gaactttgca agtcccttgg 3481 cttcggatgg ggacaaaaag ctagcagtac tatgctaccg atgtttatca ctcctctatt 3541 tgagaatgtt taagctgaag aaggaccatg ctatgaagta ctccagatca ctgatggaat 3601 attttaagca aaatgcttca aaagtcgcac agataccctc tccatgggta agcaatggaa 3661 agaacactcc atccccagtg tctctcaaca acgtctcccc catcaacgca atggggaact 3721 gtaacaatgg cccagtcacc attccccagc gcattcacca catggctgcc agccacgtca 3781 acatcactag caatgtgtta cggggctatg aacactggga tatggccgac aaactgacaa 3841 gagaaaacaa agaattcttt ggtgatctgg acacgctgat ggggcctctg acccagcaca 3901 gcagcatgac caatcttgtc cgctacgttc gccaaggact gtgttggctg cgcatcgatg 3961 cccacttgtt gttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 4021 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 4081 caatcaacct ctggatta