Construct: ORF ccsbBroad304_06338
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011781.1_s304c1, BRDN0000405743
- Derived from:
- ccsbBroadEn_06338
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GTF2A2 (2958)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06338
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2958 | GTF2A2 | general transcription facto... | NM_001320929.2 | 99.6% | 100% | 315C>T |
| 2 | human | 2958 | GTF2A2 | general transcription facto... | NM_001320930.2 | 99.6% | 100% | 315C>T |
| 3 | human | 2958 | GTF2A2 | general transcription facto... | NM_004492.3 | 99.6% | 100% | 315C>T |
| 4 | mouse | 235459 | Gtf2a2 | general transcription facto... | NM_001039519.2 | 95.1% | 99% | (many diffs) |
| 5 | mouse | 235459 | Gtf2a2 | general transcription facto... | NM_001282083.1 | 95.1% | 99% | (many diffs) |
| 6 | mouse | 235459 | Gtf2a2 | general transcription facto... | XM_006511116.2 | 95.1% | 99% | (many diffs) |
| 7 | mouse | 235459 | Gtf2a2 | general transcription facto... | NM_001282084.1 | 55.7% | 55% | (many diffs) |
| 8 | mouse | 235459 | Gtf2a2 | general transcription facto... | NR_104093.1 | 30% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 393
- ORF length:
- 327
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc atatcagtta tacagaaata ctactttggg aaacagtctt caggagagcc 121 tagatgagct catacagtct caacagatca ccccccaact tgcccttcaa gttctacttc 181 agtttgataa ggctataaat gcagcactgg ctcagagggt caggaacaga gtcaatttca 241 ggggctctct aaatacgtac agattctgcg ataatgtgtg gacttttgta ctgaatgatg 301 ttgaattcag agaggtgaca gaacttatta aagtggataa agtgaaaatt gtagcctgtg 361 atggtaaaaa tactggctct aatactacag aatgcccaac tttcttgtac aaagtggttg 421 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 481 accggtggcg cgttaagtcg acaatcaacc tctggatta