Construct: ORF ccsbBroad304_06502
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004133.1_s304c1, BRDN0000402416
- Derived from:
- ccsbBroadEn_06502
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KPNA3 (3839)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06502
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3839 | KPNA3 | karyopherin subunit alpha 3 | NM_002267.4 | 99.6% | 99.2% | (many diffs) |
| 2 | human | 3839 | KPNA3 | karyopherin subunit alpha 3 | XM_017020561.1 | 95% | 94.6% | (many diffs) |
| 3 | mouse | 16648 | Kpna3 | karyopherin (importin) alpha 3 | NM_008466.5 | 93.2% | 98.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1629
- ORF length:
- 1563
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cgagaacccc agcttggaga accaccgcat caagagcttc aagaacaagg 121 gccgcgatgt ggaaacaatg cgaagacata gaaatgaagt gacagtggaa ctgcggaaga 181 acaaaagaga tgaacactta ttgaaaaaga gaaatgttcc ccaagaagaa agtctagaag 241 attcagatgt tgatgctgat tttaaagcac aaaatgtaac cctagaagct atattgcaga 301 atgccacaag tgataaccca gtggtccaat tgagtgctgt ccaggcagca agaaaactgt 361 tatccagtga cagaaatcca ccgattgatg acttaataaa atctgggatt ttaccaattc 421 tagtcaaatg tctagaaagg gatgataatc cttcattaca gtttgaagct gcttgggcat 481 taactaacat agcatcagga acttctgcac agactcaaga tattgtgcag tctaatgcag 541 tacctctttt tctgagactt cttcgttcac cacatcagaa tgtttgtgaa caagcagtat 601 gggctttggg aaacattata ggtgatggtc ctcaatgtag agattatgtc atatcactgg 661 gagttgtcaa acctcttctg tccttcatca gtccctccat ccccatcacc ttccttcgga 721 acgtcacatg ggtcattgtc aatctctgca ggaataagga tcccccaccg cctatggaga 781 cagttcagga gattttgcca gctttatgtg tcctcatata ccatacagat ataaacattc 841 ttgtagacac tgtttgggct ctgtcatact tgacagatgg aggtaatgaa cagatacaga 901 tggttattga ttcaggagtt gtgccctttc ttgtgcccct tctgagccat caggaagtca 961 aagttcaaac agcagccctc agagcagttg gcaacatagt gactggcacc gacgagcaga 1021 cccaggttgt tctcaattgt gatgtcctgt cacacttcca aaatctctta tcacacccaa 1081 aagagaagat aaataaggaa gcagtgtggt tcctttccaa cataacagca ggcaaccagc 1141 aacaagttca agctgtaata gatgctggat taattcctat gataattcat cagcttgcta 1201 agggggactt tggaacacaa aaagaagctg cttgggcaat cagcaactta acaataagtg 1261 gcagaaaaga tcaggttgag taccttgtac agcaggatgt aataccaccg ttctgtaatt 1321 tactgtcagt gaaagattct caagtggttc aggtggttct agatggtcta aaaaacattc 1381 tgataatggc cggtgatgaa gcaagcacaa tagctgaaat aatagaggaa tgtggaggtt 1441 tggagaaaat tgaagtttta cagcaacatg aaaatgaaga catatataaa ttagcatttg 1501 aaatcataga tcagtatttc tctggtgatg atattgatga agatccctgc ctcattcctg 1561 aagcaacaca aggaggtacc tacaattttg atccaacagc caaccttcaa acaaaagaat 1621 ttaattttta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1681 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1741 tcaacctctg gatta