Construct: ORF ccsbBroad304_06584
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009354.1_s304c1, BRDN0000462053
- Derived from:
- ccsbBroadEn_06584
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MKLN1 (4289)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06584
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4289 | MKLN1 | muskelin 1 | NM_013255.5 | 99.9% | 100% | 1324C>A;1575T>C |
| 2 | human | 4289 | MKLN1 | muskelin 1 | NM_001145354.2 | 95.8% | 94.7% | (many diffs) |
| 3 | human | 4289 | MKLN1 | muskelin 1 | XM_006715993.3 | 94.5% | 94.5% | 1324C>A;1575T>C;2087_2088delCAins119 |
| 4 | human | 4289 | MKLN1 | muskelin 1 | XM_024446766.1 | 87.3% | 87.4% | 0_1ins276;1048C>A;1299T>C |
| 5 | human | 4289 | MKLN1 | muskelin 1 | XM_024446767.1 | 87.3% | 87.4% | 0_1ins276;1048C>A;1299T>C |
| 6 | human | 4289 | MKLN1 | muskelin 1 | NM_001321316.2 | 71.7% | 71.8% | 0_1ins621;703C>A;954T>C |
| 7 | human | 4289 | MKLN1 | muskelin 1 | XM_011516224.3 | 63.1% | 63.2% | 1324C>A;1396_1399delTTCC;1401_1402ins808 |
| 8 | mouse | 27418 | Mkln1 | muskelin 1, intracellular m... | NM_013791.2 | 92.9% | 99.1% | (many diffs) |
| 9 | mouse | 27418 | Mkln1 | muskelin 1, intracellular m... | XM_017321605.1 | 66.6% | 71.1% | (many diffs) |
| 10 | mouse | 27418 | Mkln1 | muskelin 1, intracellular m... | XM_017321606.1 | 51.2% | 54.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2274
- ORF length:
- 2205
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggctggc ggagctgtcg ctgcggcgcc cgagtgccgg cttctcccct 121 acgcgctaca caagtggagc tccttttcct ccacctacct tcccgagaac attttagtgg 181 acaaaccaaa tgaccaatct tcaagatggt cttcagagag caactatcct ccccagtact 241 tgattctaaa gctcgaaagg cctgctatag ttcagaatat cacatttgga aaatatgaga 301 aaactcatgt ttgcaatttg aagaaattta aagtctttgg tggaatgaat gaagaaaata 361 tgacagagct gttgtccagt ggcttaaaga atgattataa caaagaaaca ttcaccttga 421 agcataaaat tgatgaacag atgttccctt gtcgattcat taaaatagtt ccactcttgt 481 cctggggacc cagctttaac tttagcatct ggtatgttga acttagtggc attgatgatc 541 ctgatatagt acaaccttgt ctcaactggt atagcaagta ccgtgaacag gaagctattc 601 gcctttgcct aaaacacttc agacaacaca actatacaga agcttttgag tcactgcaaa 661 agaaaaccaa gattgcactg gaacatccca tgttaacaga tattcatgac aagctggtgt 721 tgaagggtga ttttgatgct tgcgaagagt tgattgaaaa ggctgtaaat gatggcttgt 781 tcaatcagta tatcagtcaa caggaatata agccacgatg gagtcaaatc attcccaaaa 841 gtaccaaagg tgatggggaa gataaccgtc caggaatgag aggaggccat cagatggtta 901 ttgatgttca aacagagact gtttatttgt ttggtggctg ggatggaaca caagatcttg 961 ctgacttctg ggcgtacagt gtgaaggaga accagtggac atgtatctct agagacactg 1021 aaaaagagaa tggtcctagt gccagatcgt gtcataaaat gtgcattgat attcaacgga 1081 ggcaaatcta cacattgggg cgttacttgg attcctctgt gaggaacagc aaatctctga 1141 aaagtgactt ctatcgttat gacattgata caaacacatg gatgttacta agtgaggata 1201 ctgctgctga tggagggccg aaattggtgt ttgatcatca gatgtgtatg gactcagaaa 1261 aacatatgat ctacactttt ggtggtagaa ttttgacttg taatggcagc gtagatgaca 1321 gcagagccag tgaaccacaa ttcagtggct tgtttgcttt caactgtcaa tgtcaaacct 1381 ggaaacttct tagagaggac tcctgtaatg ctgggcctga ggacatccag tctcgaatag 1441 gacactgcat gttattccac tcaaaaaatc gttgcttata tgtatttggt ggccagcgat 1501 caaagaccta tttgaatgat ttctttagtt atgatgtgga ctctgatcat gtagacataa 1561 tatcagatgg caccaagaaa gactctggga tggttccaat gacaggattt acacagagag 1621 caactattga tccagaactg aacgaaatac acgtcttatc tggactcagc aaagataagg 1681 aaaagaggga agaaaatgtt agaaattcat tctggattta tgacattgtg aggaatagtt 1741 ggtcttgtgt ctataagaat gatcaagctg caaaggataa tccaactaaa agtcttcagg 1801 aagaagaacc atgtccaagg tttgcccatc agcttgtata cgatgagcta cacaaggttc 1861 attacttatt tggtgggaat ccaggaaaat cttgctctcc aaagatgaga ttagatgact 1921 tctggtcact gaagttgtgt agaccttcaa aagattattt actgaggcat tgcaagtacc 1981 tcataagaaa acacaggttt gaagaaaagg cccaagtgga tccccttagt gctctgaaat 2041 atttacaaaa tgatctttat ataactgtgg atcattcaga cccagaagag acaaaagagt 2101 ttcagctcct ggcatcagct ctattcaaat ctggttcaga ttttacagct ctgggctttt 2161 ctgatgtgga tcacacctat gctcaaagaa ctcagctctt tgacacctta gtaaatttct 2221 ttcctgacag catgactcct cctaaaggca acctggtaga cctcatcaca ctgttgccaa 2281 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 2341 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta