Construct: ORF ccsbBroad304_06637
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002616.1_s304c1, BRDN0000410843
- Derived from:
- ccsbBroadEn_06637
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NFKB1 (4790)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06637
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4790 | NFKB1 | nuclear factor kappa B subu... | NM_003998.4 | 87.5% | 82.7% | (many diffs) |
| 2 | human | 4790 | NFKB1 | nuclear factor kappa B subu... | XM_024454068.1 | 87.5% | 82.7% | (many diffs) |
| 3 | human | 4790 | NFKB1 | nuclear factor kappa B subu... | NM_001165412.2 | 87.4% | 82.5% | (many diffs) |
| 4 | human | 4790 | NFKB1 | nuclear factor kappa B subu... | NM_001319226.2 | 87.4% | 82.5% | (many diffs) |
| 5 | human | 4790 | NFKB1 | nuclear factor kappa B subu... | XM_024454067.1 | 86.8% | 82% | (many diffs) |
| 6 | human | 4790 | NFKB1 | nuclear factor kappa B subu... | XM_011532006.2 | 86.7% | 81.9% | (many diffs) |
| 7 | human | 4790 | NFKB1 | nuclear factor kappa B subu... | XM_024454069.1 | 81.6% | 76.5% | (many diffs) |
| 8 | mouse | 18033 | Nfkb1 | nuclear factor of kappa lig... | XM_006501106.2 | 77% | 77.4% | (many diffs) |
| 9 | mouse | 18033 | Nfkb1 | nuclear factor of kappa lig... | NM_008689.2 | 74.7% | 71.4% | (many diffs) |
| 10 | mouse | 18033 | Nfkb1 | nuclear factor of kappa lig... | XM_006501107.1 | 63.5% | 60.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2532
- ORF length:
- 2466
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc agaagatgat ccatatttgg gaaggcctga acaaatgttt catttggatc 121 cttctttgac tcatacaata tttaatccag aagtatttca accacagatg gcactgccaa 181 cagcagatgg cccatacctt caaatattag agcaacctaa acagagagga tttcgtttcc 241 gttatgtatg tgaaggccca tcccatggtg gactacctgg tgcctctagt gaaaagaaca 301 agaagtctta ccctcaggtc aaaatctgca actatgtggg accagcaaag gttattgttc 361 agttggtcac aaatggaaaa aatatccacc tgcatgccca cagcctggtg ggaaaacact 421 gtgaggatgg gatctgcact gtaactgctg gacccaagga catggtggtc ggcttcgcaa 481 acctgggtat acttcatgtg acaaagaaaa aagtatttga aacactggaa gcacgaatga 541 cagaggcgtg tataaggggc tataatcctg gactcttggt gcaccctgac cttgcctatt 601 tgcaagcaga aggtggaggg gaccggcagc tgggagatcg ggaaaaagag ctaatccgcc 661 aagcagctct gcagcagacc aaggagatgg acctcagcgt ggtgcggctc atgtttacag 721 cttttcttcc ggatagcact ggcagcttca caaggcgcct ggaacccgtg gtatcagacg 781 ccatctatga cagtaaagcc cccaatgcat ccaacttgaa aattgtaaga atggacagga 841 cagctggatg tgtgactgga ggggaggaaa tttatcttct ttgtgacaaa gttcagaaag 901 atgacatcca gattcgattt tatgaagagg aagaaaatgg tggagtctgg gaaggatttg 961 gagatttttc ccccacagat gttcatagac aatttgccat tgtcttcaaa actccaaagt 1021 ataaagatat taatattaca aaaccagcct ctgtgtttgt ccagcttcgg aggaaatctg 1081 acttggaaac tagtgaacca aaacctttcc tctactatcc tgaaatcaaa gataaagaag 1141 aagtgcagag gaaacgtcag aagctcatgc ccaatttttc ggatagtttc ggcggtggta 1201 gtggtgccgg agctggaggc ggaggcatgt ttggtagtgg cggtggagga gggggcactg 1261 gaagtacagg tccagggtat agcttcccac actatggatt tcctacttat ggtgggatta 1321 ctttccatcc tggaactact aaatctaatg ctgggatgaa gcatggaacc atggacactg 1381 aatctaaaaa ggaccctgaa ggttgtgaca aaagtgatga caaaaacact gtaaacctct 1441 ttgggaaagt tattgaaacc acagagcaag atcaggagcc cagcgaggcc accgttggga 1501 atggtgaggt cactctaacg tatgcaacag gaacaaaaga agagagtgct ggagttcagg 1561 ataacctctt tctagagaag gctatgcagc ttgcaaagag gcatgccaat gcccttttcg 1621 actacgcggt gacaggagac gtgaagatgc tgctggccgt ccagcgccat ctcactgctg 1681 tgcaggatga gaatggggac agtgtcttac acttagcaat catccacctt cattctcaac 1741 ttgtgaggga tctactagaa gtcacatctg gtttgatttc tgatgacatt atcaacatga 1801 gaaatgatct gtaccagacg cccttgcact tggcagtgat cactaagcag gaagatgtgg 1861 tggaggattt gctgagggct ggggccgacc tgagccttct ggaccgcttg ggtaactctg 1921 ttttgcacct agctgccaaa gaaggacatg ataaagttct cagtatctta ctcaagcaca 1981 aaaaggcagc actacttctt gaccacccca acggggacgg tctgaatgcc attcatctag 2041 ccatgatgag caatagcctg ccatgtttgc tgctgctggt ggccgctggg gctgacgtca 2101 atgctcagga gcagaagtcc gggcgcacag cactgcacct ggctgtggag cacgacaaca 2161 tctcattggc aggctgcctg ctcctggaag gtgatgccca tgtggacagt actacctacg 2221 atggaaccac acccctgcat atagcagctg ggagagggtc caccaggctg gcagctcttc 2281 tcaaagcagc aggagcagat cccctggtgg agaactttga gcctctctat gacctggatg 2341 actcttggga aaatgcagga gaggatgaag gagttgtgcc tggaaccacg cctctagata 2401 tggccaccag ctggcaggta tttgacatat taaatgggaa accatatgag ccagagttta 2461 catctgatGT TTTTCTTGGA CTTGCCGAAA GAGCCTTAAC AGGCTGGAAG GTGAACTCTC 2521 CACTCCAACT GTAACAGTGA AACTGCAACG TCTTCTTTGG TAGTTGGATG ATGTTCTTGG 2581 ATTTAACGCG AAAGAATCAC ACGAAAGGAA CAAAATGAGA CGGGAAGTCC AACATCATTC 2641 TCTTTTACGA AAAGCATAGA AACATGTGCC TCTATAGTGT AAAATACCCT TCACCCAAAC 2701 GAAAATCTTC CAGGAAAATT GTTTATTTCT CACCGGGCTA TTCATGGACC ACACAATAAA 2761 CAGGATGAAA GACACGCAAC GGTAAAATAA TTGGCCTGGA GATCCAGAGT GGGTTCCTGG 2821 GGTACCGATG TTACATTGCC AACAGTTTTT ATAATGTAGG TGTAATTCGA CCCTATCCCT 2881 AACTGTTGTT TGCACATGGA AGAGTCACCG ATGTTAGGTC ACCCGTGGGT GGGTTAGGCG 2941 GCCAACAACC TTATTATTAC AAACATTGTG AAAAAATGAG GGGTCTTCAT TAATAATTTG 3001 GTCCTTCCAG CTATGTGGGG ACGCGGATGA AAGCCATTGG GTGAAGTTAT TCCCTTGAA