Construct: ORF ccsbBroad304_06703

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013795.1_s304c1, BRDN0000461752
Derived from:
ccsbBroadEn_06703
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDE4B (5142)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06703
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5142 PDE4B phosphodiesterase 4B NM_001037341.1 99.9% 99.8% 315G>N
2 human 5142 PDE4B phosphodiesterase 4B NM_002600.4 99.9% 99.8% 315G>N
3 human 5142 PDE4B phosphodiesterase 4B NM_001037340.2 91.8% 88.9% (many diffs)
4 human 5142 PDE4B phosphodiesterase 4B NM_001297441.1 89.5% 89.1% (many diffs)
5 human 5142 PDE4B phosphodiesterase 4B NM_001297440.1 87.3% 87% (many diffs)
6 human 5142 PDE4B phosphodiesterase 4B XM_017001445.1 79.3% 77.7% (many diffs)
7 human 5142 PDE4B phosphodiesterase 4B NM_001037339.2 73% 71.8% (many diffs)
8 human 5142 PDE4B phosphodiesterase 4B XM_006710680.3 71.6% 71.3% (many diffs)
9 human 5142 PDE4B phosphodiesterase 4B XM_005270924.3 70.2% 70.2% 0_1ins657
10 human 5142 PDE4B phosphodiesterase 4B XM_005270925.2 70.2% 70.2% 0_1ins657
11 human 5142 PDE4B phosphodiesterase 4B NM_001297442.2 67.5% 66.9% (many diffs)
12 mouse 18578 Pde4b phosphodiesterase 4B, cAMP ... XM_006502855.3 89.4% 97% (many diffs)
13 mouse 18578 Pde4b phosphodiesterase 4B, cAMP ... NM_019840.2 82.3% 86.5% (many diffs)
14 mouse 18578 Pde4b phosphodiesterase 4B, cAMP ... XM_006502856.2 81.3% 81.9% (many diffs)
15 mouse 18578 Pde4b phosphodiesterase 4B, cAMP ... XM_006502857.3 79.7% 85.4% (many diffs)
16 mouse 18578 Pde4b phosphodiesterase 4B, cAMP ... NM_001177980.1 67.3% 71.2% (many diffs)
17 mouse 18578 Pde4b phosphodiesterase 4B, cAMP ... XM_017320029.1 64.6% 70.5% (many diffs)
18 mouse 18578 Pde4b phosphodiesterase 4B, cAMP ... XM_017320030.1 63.2% 69.1% (many diffs)
19 mouse 18578 Pde4b phosphodiesterase 4B, cAMP ... NM_001177981.1 61% 65.8% (many diffs)
20 mouse 18578 Pde4b phosphodiesterase 4B, cAMP ... NM_001177982.1 59.1% 64.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2277
ORF length:
2208
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaagaaaagc aggagtgtga tgacggtgat ggctgatgat aatgttaaag
121attattttga atgtagcttg agtaaatcct acagttcttc cagtaacaca cttgggatcg
181acctctggag agggagaagg tgttgctcag gaaacttaca gttaccacca ctgtctcaaa
241gacagagtga aagggcaagg actcctgagg gagatggtat ttccaggccg accacactgc
301ctttgacaac gcttccaagc attgctatta caactgtaag ccaggagtgc tttgatgtgg
361aaaatggccc ttccccaggt cgnagtccac tggatcccca ggccagctct tccgctgggc
421tggtacttca cgccaccttt cctgggcaca gccagcgcag agagtcattt ctctacagat
481cagacagcga ctatgacttg tcaccaaagg cgatgtcgag aaactcttct cttccaagcg
541agcaacacgg cgatgacttg attgtaactc cttttgccca ggtccttgcc agcttgcgaa
601gtgtgagaaa caacttcact atactgacaa accttcatgg tacatctaac aagaggtccc
661cagctgctag tcagcctcct gtctccagag tcaacccaca agaagaatct tatcaaaaat
721tagcaatgga aacgctggag gaattagact ggtgtttaga ccagctagag accatacaga
781cctaccggtc tgtcagtgag atggcttcta acaagttcaa aagaatgctg aaccgggagc
841tgacacacct ctcagagatg agccgatcag ggaaccaggt gtctgaatac atttcaaata
901ctttcttaga caagcagaat gatgtggaga tcccatctcc tacccagaaa gacagggaga
961aaaagaaaaa gcagcagctc atgacccaga taagtggagt gaagaaatta atgcatagtt
1021caagcctaaa caatacaagc atctcacgct ttggagtcaa cactgaaaat gaagatcacc
1081tggccaagga gctggaagac ctgaacaaat ggggtcttaa catctttaat gtggctggat
1141attctcacaa tagaccccta acatgcatca tgtatgctat attccaggaa agagacctcc
1201taaagacatt cagaatctca tctgacacat ttataaccta catgatgact ttagaagacc
1261attaccattc tgacgtggca tatcacaaca gcctgcacgc tgctgatgta gcccagtcga
1321cccatgttct cctttctaca ccagcattag acgctgtctt cacagatttg gagatcctgg
1381ctgccatttt tgcagctgcc atccatgacg ttgatcatcc tggagtctcc aatcagtttc
1441tcatcaacac aaattcagaa cttgctttga tgtataatga tgaatctgtg ttggaaaatc
1501atcaccttgc tgtgggtttc aaactgctgc aagaagaaca ctgtgacatc ttcatgaatc
1561tcaccaagaa gcagcgtcag acactcagga agatggttat tgacatggtg ttagcaactg
1621atatgtctaa acatatgagc ctgctggcag acctgaagac aatggtagaa acgaagaaag
1681ttacaagttc aggcgttctt ctcctagaca actataccga tcgcattcag gtccttcgca
1741acatggtaca ctgtgcagac ctgagcaacc ccaccaagtc cttggaattg tatcggcaat
1801ggacagaccg catcatggag gaatttttcc agcagggaga caaagagcgg gagaggggaa
1861tggaaattag cccaatgtgt gataaacaca cagcttctgt ggaaaaatcc caggttggtt
1921tcatcgacta cattgtccat ccattgtggg agacatgggc agatttggta cagcctgatg
1981ctcaggacat tctcgatacc ttagaagata acaggaactg gtatcagagc atgatacctc
2041aaagtccctc accaccactg gacgagcaga acagggactg ccagggtctg atggagaagt
2101ttcagtttga actgactctc gatgaggaag attctgaagg acctgagaag gagggagagg
2161gacacagcta tttcagcagc acaaagacgc tttgtgtgat tgatccagaa aacagagatt
2221ccctgggaga gactgacata gacattgcaa cagaagacaa gtcccccgtg gatacattgc
2281caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
2341ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
2401tta

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