Construct: ORF ccsbBroad304_06763
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017627.1_s304c1, BRDN0000402055
- Derived from:
- ccsbBroadEn_06763
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRB1 (5542)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06763
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5545 | PRB4 | proline rich protein BstNI ... | NM_001261399.2 | 73.1% | 62.4% | (many diffs) |
| 2 | human | 5545 | PRB4 | proline rich protein BstNI ... | NM_002723.6 | 72.3% | 63.1% | (many diffs) |
| 3 | human | 5542 | PRB1 | proline rich protein BstNI ... | NM_005039.3 | 59.5% | 59.2% | (many diffs) |
| 4 | human | 5544 | PRB3 | proline rich protein BstNI ... | NM_006249.5 | 57.6% | 50.1% | (many diffs) |
| 5 | human | 5542 | PRB1 | proline rich protein BstNI ... | NR_160307.1 | 50.2% | (many diffs) | |
| 6 | human | 653247 | PRB2 | proline rich protein BstNI ... | NM_006248.4 | 46.7% | 46.1% | (many diffs) |
| 7 | human | 5542 | PRB1 | proline rich protein BstNI ... | NM_001367912.1 | 30.4% | 29.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 663
- ORF length:
- 594
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgttgatt ctgctgtcag tggccttgct ggccctgagc tcagctcaga 121 acttaaatga agatgtcagc caggaagaat ctccctccct aatagcagga aatccacaag 181 gaccatcccc acaaggaggc aacaagcccc aaggcccccc acctcctcca ggaaagccac 241 aaggaccacc cccacaagga ggcaacaaac ctcaaggtcc cccacctcca ggaaagccac 301 aaggaccacc cccacaaggg gacaagtccc gaagtccccg atctcctcca ggaaaaccac 361 aaggaccacc cccacaagga ggaaagccac aaggaccacc cccacaagga ggcaacaaac 421 ctcaaggtcc cctacctcca ggaaagccac aaggaccacc cgcacaagga ggcagcaagt 481 cccaaagtgc ccgagctcct ccaggaaagc cacaaggacc accccaacaa gaaggcaaca 541 atcctcaagg tcccccacct ccagcaggag gcaatcccca gcagcctcag gcacctcctg 601 ctggacagcc ccagggacca ccacgccctc ctcaaggggg cagaccttcc agacctcccc 661 agttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 721 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 781 tctggatta