Construct: ORF ccsbBroad304_06862
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013983.1_s304c1, BRDN0000404875
- Derived from:
- ccsbBroadEn_06862
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGS3 (5998)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06862
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5998 | RGS3 | regulator of G protein sign... | NM_130795.4 | 99.9% | 99.8% | 1331A>G |
| 2 | human | 5998 | RGS3 | regulator of G protein sign... | NM_001282923.2 | 83.8% | 83% | (many diffs) |
| 3 | human | 5998 | RGS3 | regulator of G protein sign... | NM_144488.6 | 76.1% | 75.4% | (many diffs) |
| 4 | human | 5998 | RGS3 | regulator of G protein sign... | NM_001276261.1 | 64.4% | 64.4% | 1191_1192ins978 |
| 5 | human | 5998 | RGS3 | regulator of G protein sign... | NM_001322215.2 | 58.1% | 56.8% | (many diffs) |
| 6 | human | 5998 | RGS3 | regulator of G protein sign... | NM_001276260.2 | 56.5% | 56.4% | 0_1ins1194;137A>G |
| 7 | human | 5998 | RGS3 | regulator of G protein sign... | NM_001282922.2 | 56.5% | 56.4% | 0_1ins1194;137A>G |
| 8 | human | 5998 | RGS3 | regulator of G protein sign... | NM_001351526.2 | 56.5% | 56.4% | 0_1ins1194;137A>G |
| 9 | human | 5998 | RGS3 | regulator of G protein sign... | NM_001322214.1 | 45.3% | 44.2% | (many diffs) |
| 10 | human | 5998 | RGS3 | regulator of G protein sign... | NM_017790.4 | 38% | 36.3% | (many diffs) |
| 11 | human | 5998 | RGS3 | regulator of G protein sign... | NM_144489.3 | 29.9% | 22.1% | (many diffs) |
| 12 | human | 5998 | RGS3 | regulator of G protein sign... | NM_001276262.2 | 18.3% | 18.3% | 0_1ins2247 |
| 13 | human | 5998 | RGS3 | regulator of G protein sign... | NM_134427.2 | 18.3% | 18.3% | 0_1ins2247 |
| 14 | mouse | 50780 | Rgs3 | regulator of G-protein sign... | NM_134257.3 | 79.9% | 79.6% | (many diffs) |
| 15 | mouse | 50780 | Rgs3 | regulator of G-protein sign... | XM_006538060.3 | 70.9% | 69% | (many diffs) |
| 16 | mouse | 50780 | Rgs3 | regulator of G-protein sign... | XM_006538058.3 | 67.8% | 66% | (many diffs) |
| 17 | mouse | 50780 | Rgs3 | regulator of G-protein sign... | XM_006538057.3 | 67.3% | 65.6% | (many diffs) |
| 18 | mouse | 50780 | Rgs3 | regulator of G-protein sign... | XM_017320307.1 | 67.3% | 65.6% | (many diffs) |
| 19 | mouse | 50780 | Rgs3 | regulator of G-protein sign... | NM_001081650.2 | 41.2% | 41% | (many diffs) |
| 20 | mouse | 50780 | Rgs3 | regulator of G-protein sign... | XM_006538064.3 | 34.5% | 33% | (many diffs) |
| 21 | mouse | 50780 | Rgs3 | regulator of G-protein sign... | NM_019492.3 | 34.2% | 32.7% | (many diffs) |
| 22 | mouse | 50780 | Rgs3 | regulator of G-protein sign... | XM_011250065.2 | 31.4% | 28.2% | (many diffs) |
| 23 | mouse | 50780 | Rgs3 | regulator of G-protein sign... | XM_006538072.3 | 15.8% | 16.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2817
- ORF length:
- 2751
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccgcttcaat gggctctgca aggtgtgctc ggagcgccgc taccgccaga 121 tcaccatccc gaggggaaag gacggctttg gcttcaccat ctgctgcgac tctccagttc 181 gagtccaggc cgtggattcc gggggtccgg cggaacgggc agggctgcag cagctggaca 241 cggtgctgca gctgaatgag aggcctgtgg agcactggaa atgtgtggag ctggcccacg 301 agatccggag ctgccccagt gagatcatcc tactcgtgtg gcgcatggtc ccccaggtca 361 agccaggacc agatggcggg gtcctgcggc gggcctcctg caagtcgaca catgacctcc 421 agtcaccccc caacaaacgg gagaagaact gcacccatgg ggtccaggca cggcctgagc 481 agcgccacag ctgccacctg gtatgtgaca gctctgatgg gctgctgctc ggcggctggg 541 agcgctacac cgaggtggcc aagcgcgggg gccagcacac cctgcctgca ctgtcccgtg 601 ccactgcccc caccgacccc aactacatca tcctggcccc gctgaatcct gggagccagc 661 tgctccggcc tgtgtaccag gaggatacca tccccgaaga atcagggagt cccagtaaag 721 ggaagtccta cacaggcctg gggaagaagt cccggctgat gaagacagtg cagaccatga 781 agggccacgg gaactaccaa aactgcccgg ttgtgaggcc gcatgccacg cactcaagct 841 atggcaccta cgtcaccctg gcccccaaag tcctggtgtt ccctgtcttt gttcagcctc 901 tagatctctg taatcctgcc cggaccctcc tgctgtcaga ggagctgctg ctgtatgaag 961 ggaggaacaa ggctgccgag gtgacactgt ttgcctattc ggacctgctg ctcttcacca 1021 aggaggacga gcctggccgc tgcgacgtcc tgaggaaccc cctctacctc cagagtgtga 1081 agctgcagga aggttcttca gaagacctga aattctgcgt gctctatcta gcagagaagg 1141 cagagtgctt attcactttg gaagcgcact cgcaggagca gaagaagaga gtgtgctggt 1201 gcctgtcgga gaacatcgcc aagcagcaac agctggcagc atcacccccg gacagcaaga 1261 tgtttgagac ggaggcagat gagaagaggg agatggcctt ggaggaaggg aaggggcctg 1321 gtgccgagga ttccccaccc agcaaggagc cctctcctgg ccaggagctt cctccaggac 1381 aagaccttcc acccagcaag gactcccctt ctgggcagga acccgctccc agccaagaac 1441 cactgtccag caaagactca gctacctctg aaggatcccc tccaggccca gatgctccgc 1501 ccagcaagga tgtgccacca tgccaggaac cccctccagc ccaagacctc tcaccctgcc 1561 aggacctacc tgctggtcaa gaacccctgc ctcaccagga ccctctactc accaaagacc 1621 tccctgccat ccaggaatcc cccacccggg accttccacc ctgtcaagat ctgcctccta 1681 gccaggtctc cctgccagcc aaggccctta ctgaggacac catgagctcc ggggacctac 1741 tagcagctac tggggaccca cctgcggccc ccaggccagc cttcgtgatc cctgaggtcc 1801 ggctggatag cacctacagc cagaaggcag gggcagagca gggctgctcg ggagatgagg 1861 aggatgcaga agaggccgag gaggtggagg agggggagga aggggaggag gacgaggatg 1921 aggacaccag cgatgacaac tacggagagc gcagtgaggc caagcgcagc agcatgatcg 1981 agacgggcca gggggctgag ggtggcctct cactgcgtgt gcagaactcg ctgcggcgcc 2041 ggacgcacag cgagggcagc ctgctgcagg agccccgagg gccctgcttt gcctccgaca 2101 ccaccttgca ctgctcagac ggtgagggcg ccgcctccac ctggggcatg ccttcgccca 2161 gcaccctcaa gaaagagctg ggccgcaatg gtggctccat gcaccacctt tccctcttct 2221 tcacaggaca caggaagatg agcggggctg acaccgttgg ggatgatgac gaagcctccc 2281 ggaagagaaa gagcaaaaac ctagccaagg acatgaagaa caagctgggg atcttcagac 2341 ggcggaatga gtcccctgga gcccctcccg cgggcaaggc agacaaaatg atgaagtcat 2401 tcaagcccac ctcagaggaa gccctcaagt ggggcgagtc cttggagaag ctgctggttc 2461 acaaatacgg gttagcagtg ttccaagcct tccttcgcac tgagttcagt gaggagaatc 2521 tggagttctg gttggcttgt gaggacttca agaaggtcaa gtcacagtcc aagatggcat 2581 ccaaggccaa gaagatcttt gctgaataca tcgcgatcca ggcatgcaag gaggtcaacc 2641 tggactccta cacgcgggag cacaccaagg acaacctgca gagcgtcacg cggggctgct 2701 tcgacctggc acagaagcgc atcttcgggc tcatggaaaa ggactcgtac cctcgctttc 2761 tccgttctga cctctacctg gaccttatta accagaagaa gatgagtccc ccgctttacc 2821 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 2881 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 2941 tta