Construct: ORF ccsbBroad304_06864
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010788.1_s304c1, BRDN0000410686
- Derived from:
- ccsbBroadEn_06864
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RHEB (6009)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06864
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6009 | RHEB | Ras homolog, mTORC1 binding | NM_005614.4 | 99.8% | 99.4% | 257C>T |
| 2 | human | 6009 | RHEB | Ras homolog, mTORC1 binding | XM_011516457.2 | 91.3% | 90.7% | (many diffs) |
| 3 | human | 6009 | RHEB | Ras homolog, mTORC1 binding | XM_024446854.1 | 91.3% | 90.7% | (many diffs) |
| 4 | human | 439965 | SHLD2P3 | shieldin complex subunit 2 ... | NR_160660.1 | 23.1% | (many diffs) | |
| 5 | human | 439965 | SHLD2P3 | shieldin complex subunit 2 ... | NR_160661.1 | 21.2% | (many diffs) | |
| 6 | mouse | 19744 | Rheb | Ras homolog enriched in brain | NM_053075.3 | 92.3% | 98.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 618
- ORF length:
- 552
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcATGCC GCAGTCCAAG TCCCGGAAGA TCGCGATCCT GGGCTACCGG TCTGTGGGGA 121 AATCCTCATT GACGATTCAA TTTGTTGAAG GCCAATTTGT GGACTCCTAC GATCCAACCA 181 TAGAAAACAC TTTTACAAAG TTGATCACAG TAAATGGACA AGAATATCAT CTTCAACTTG 241 TAGACACAGC CGGGCAAGAT GAATATTCTA TCTTTCCTCA GACATACTCC ATAGATATTA 301 ATGGCTATAT TCTTGTGTAT TTTGTTACAT CAATCAAAAG TTTTGAAGTG ATTAAAGTTA 361 TCCATGGCAA ATTGTTGGAT ATGGTGGGGA AAGTACAAAT ACCTATTATG TTGGTTGGGA 421 ATAAGAAAGA CCTGCATATG GAAAGGGTGA TCAGTTATGA AGAAGGGAAA GCTTTGGCAG 481 AATCTTGGAA TGCAGCTTTT TTGGAATCTT CTGCTAAAGA AAATCAGACT GCTGTGGATG 541 TTTTTCGAAG GATAATTTTG GAGGCAGAAA AAATGGACGG GGCAGCTTCA CAAGGCAAGT 601 CTTCATGCTC GGTGATGTGC CCAACTTTCT TGTACaaagt ggttggtaag cctatcccta 661 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 721 agtcgacaat caacctctgg atta