Construct: ORF ccsbBroad304_06916
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015406.1_s304c1, BRDN0000409728
- Derived from:
- ccsbBroadEn_06916
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCP2 (6342)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06916
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6342 | SCP2 | sterol carrier protein 2 | NM_001007099.3 | 99.7% | 99.3% | 422C>G |
| 2 | human | 6342 | SCP2 | sterol carrier protein 2 | NM_001007100.3 | 97.6% | 97.2% | 21_22insAGTTCTTTT;413C>G |
| 3 | human | 6342 | SCP2 | sterol carrier protein 2 | NM_001007250.2 | 41.2% | 29% | 124_125ins130;177_178ins122 |
| 4 | human | 6342 | SCP2 | sterol carrier protein 2 | NM_001193617.2 | 30.6% | 30.4% | 1_969del;1391C>G |
| 5 | human | 6342 | SCP2 | sterol carrier protein 2 | NM_001193600.2 | 28.3% | 28.2% | 1_1080del;1502C>G |
| 6 | human | 6342 | SCP2 | sterol carrier protein 2 | NM_001193599.2 | 27.2% | 27.1% | 1_1140del;1562C>G |
| 7 | human | 6342 | SCP2 | sterol carrier protein 2 | NM_002979.5 | 26% | 25.9% | 1_1212del;1634C>G |
| 8 | mouse | 20280 | Scp2 | sterol carrier protein 2, l... | XM_011240477.2 | 23.5% | 25.4% | (many diffs) |
| 9 | mouse | 20280 | Scp2 | sterol carrier protein 2, l... | NM_011327.4 | 22.8% | 24.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 495
- ORF length:
- 429
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ttttccggaa gccgccagtt cttttagaac tcatcaaatt gaagctgttc 121 caaccagctc tgcaagtgat ggatttaagg caaatcttgt ttttaaggag attgagaaga 181 aacttgaaga ggaaggggaa cagtttgtga agaaaatcgg tggtattttt gccttcaagg 241 tgaaagatgg ccctgggggt aaagaggcca cctgggtggt ggatgtgaag aatggcaaag 301 gatcagtgct tcctaactca gataagaagg ctgactgcac aatcacaatg gctgactcag 361 acttcctggc tttaatgact ggtaaaatga atcctcagtc ggccttcttt caaggcaaat 421 tgaaaatcac tggcaacatg ggtctcgcta tgaagttaca aaatcttcag cttcagccag 481 gcaacggtaa gctctgccaa ctttcttgta caaagtggtt ggtaagccta tccctaaccc 541 tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc gcgttaagtc 601 gacaatcaac ctctggatta