Construct: ORF ccsbBroad304_06955

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007500.1_s304c1, BRDN0000461550
Derived from:
ccsbBroadEn_06955
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC1A3 (6507)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06955
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6507 SLC1A3 solute carrier family 1 mem... NM_004172.5 99.8% 99.8% 67C>A;1446C>A
2 human 6507 SLC1A3 solute carrier family 1 mem... XM_005248342.3 99.8% 99.8% 67C>A;1446C>A
3 human 6507 SLC1A3 solute carrier family 1 mem... XM_024446181.1 99.8% 99.8% 67C>A;1446C>A
4 human 6507 SLC1A3 solute carrier family 1 mem... NM_001166695.3 91.5% 91.3% 67C>A;1289_1290ins135;1311C>A
5 human 6507 SLC1A3 solute carrier family 1 mem... XM_024446182.1 91.5% 91.3% 67C>A;1289_1290ins135;1311C>A
6 human 6507 SLC1A3 solute carrier family 1 mem... NM_001289939.1 91.3% 91.3% 67C>A;180_181ins138;1308C>A
7 human 6507 SLC1A3 solute carrier family 1 mem... XM_011514084.2 80.1% 80.2% 0_1ins321;1125C>A
8 human 6507 SLC1A3 solute carrier family 1 mem... NM_001289940.1 79.2% 79.1% 67C>A;523_524ins336;1110C>A
9 human 6507 SLC1A3 solute carrier family 1 mem... NM_001166696.2 11.6% 11% (many diffs)
10 mouse 20512 Slc1a3 solute carrier family 1 (gl... NM_148938.3 88.8% 96.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
65
ORF end:
1691
ORF length:
1626
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaagaaa
61gttaatgact aaaagcaatg gagaagagcc caagatgggg ggcaggatgg agagattcca
121gcagggagtc agtaaacgca cacttttggc caagaagaaa gtgcagaaca ttacaaagga
181ggatgttaaa agttacctgt ttcggaatgc ttttgtgctg ctcacagtca ccgctgtcat
241tgtgggtaca atccttggat ttaccctccg accatacaga atgagctacc gggaagtcaa
301gtacttctcc tttcctgggg aacttctgat gaggatgtta cagatgctgg tcttaccact
361tatcatctcc agtcttgtca caggaatggc ggcgctagat agtaaggcat cagggaagat
421gggaatgcga gctgtagtct attatatgac taccaccatc attgctgtgg tgattggcat
481aatcattgtc atcatcatcc atcctgggaa gggcacaaag gaaaacatgc acagagaagg
541caaaattgta cgagtgacag ctgcagatgc cttcctggac ttgatcagga acatgttccc
601tccaaatctg gtagaagcct gctttaaaca gtttaaaacc aactatgaga agagaagctt
661taaagtgccc atccaggcca acgaaacgct tgtgggtgct gtgataaaca atgtgtctga
721ggccatggag actcttaccc gaatcacaga ggagctggtc ccagttccag gatctgtgaa
781tggagtcaat gccctgggtc tagttgtctt ctccatgtgc ttcggttttg tgattggaaa
841catgaaggaa caggggcagg ccctgagaga gttctttgat tctcttaacg aagccatcat
901gagactggta gcagtaataa tgtggtatgc ccccgtgggt attctcttcc tgattgctgg
961gaagattgtg gagatggaag acatgggtgt gattgggggg cagcttgcca tgtacaccgt
1021gactgtcatt gttggcttac tcattcacgc agtcatcgtc ttgccactcc tctacttctt
1081ggtaacacgg aaaaaccctt gggtttttat tggagggttg ctgcaagcac tcatcaccgc
1141tctggggacc tcttcaagtt ctgccaccct acccatcacc ttcaagtgcc tggaagagaa
1201caatggcgtg gacaagcgcg tcaccagatt cgtgctcccc gtaggagcca ccattaacat
1261ggatgggact gccctctatg aggctttggc tgccattttc attgctcaag ttaacaactt
1321tgaactgaac ttcggacaaa ttattacaat cagcatcaca gccacagctg ccagtattgg
1381ggcagctgga attcctcagg cgggcctggt cactatggtc attgtgctga catctgtcgg
1441cctgcccact gacgacatca cgctcatcat cgcggtggac tggttcctgg atcgcctccg
1501gaccaccaca aacgtactgg gagactccct gggagctggg attgtggagc acttgtcacg
1561acatgaactg aagaacagag atgttgaaat gggtaactca gtgattgaag agaatgaaat
1621gaagaaacca tatcaactga ttgcacagga caatgaaact gagaaaccca tcgacagtga
1681aaccaagatg tacccaactt tcttgtacaa agtggttggt aagcctatcc ctaaccctct
1741cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac
1801aatcaacctc tggatta

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