Construct: ORF ccsbBroad304_07131
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006390.1_s304c1, BRDN0000461266
- Derived from:
- ccsbBroadEn_07131
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VRK1 (7443)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07131
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | NM_003384.3 | 99.8% | 100% | 516G>A;705C>T |
| 2 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XM_017021624.2 | 99.5% | 99.7% | 516G>A;705C>T;1065_1066insAAG |
| 3 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XM_017021625.1 | 99.3% | 99.4% | 1_6delATGAAA;522G>A;711C>T |
| 4 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XM_006720247.4 | 94.1% | 94.2% | 516G>A;705C>T;1160_1231del |
| 5 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XM_011537132.1 | 93.8% | 94% | (many diffs) |
| 6 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XR_001750539.2 | 64% | (many diffs) | |
| 7 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XM_017021626.2 | 61.4% | 59.8% | (many diffs) |
| 8 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | NM_001029844.1 | 84.3% | 87.1% | (many diffs) |
| 9 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_017315046.1 | 84.3% | 87.1% | (many diffs) |
| 10 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | NM_001029843.1 | 80.6% | 82.9% | (many diffs) |
| 11 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_011244103.1 | 79.7% | 82.1% | (many diffs) |
| 12 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | NM_011705.3 | 76.2% | 78.4% | (many diffs) |
| 13 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_006515809.2 | 76.2% | 78.4% | (many diffs) |
| 14 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_006515810.3 | 76.2% | 78.4% | (many diffs) |
| 15 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_006515811.3 | 76.2% | 78.4% | (many diffs) |
| 16 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_006515812.2 | 76.2% | 78.4% | (many diffs) |
| 17 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_017315045.1 | 76% | 78.1% | (many diffs) |
| 18 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_011244104.2 | 51.5% | 52.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1257
- ORF length:
- 1188
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctcgtgta aaagcagctc aagctggaag acagagctct gcaaagagac 121 atcttgcaga acaatttgca gttggagaga taataactga catggcaaaa aaggaatgga 181 aagtaggatt acccattggc caaggaggct ttggctgtat atatcttgct gatatgaatt 241 cttcagagtc agttggcagt gatgcacctt gtgttgtaaa agtggaaccc agtgacaatg 301 gacctctttt tactgaatta aagttctacc aacgagctgc aaaaccagag caaattcaga 361 aatggattcg tacccgtaag ctgaagtacc tgggtgttcc taagtattgg gggtctggtc 421 tacatgacaa aaatggaaaa agttacaggt ttatgataat ggatcgcttt gggagtgacc 481 ttcagaaaat atatgaagca aatgccaaaa ggttttctcg gaaaactgtc ttgcagctaa 541 gcttaagaat tctggatatt ctggaatata ttcacgagca tgaatatgtg catggagata 601 tcaaggcctc aaatcttctt ctgaactaca agaatcctga ccaggtgtac ttggtagatt 661 atggccttgc ttatcggtac tgcccagaag gagttcataa agaatacaaa gaagacccca 721 aaagatgtca cgatggcact attgaattca cgagcatcga tgcacacaat ggtgtggccc 781 catcaagacg tggtgatttg gaaatacttg gttattgcat gatccaatgg cttactggcc 841 atcttccttg ggaggataat ttgaaagatc ctaaatatgt tagagattcc aaaattagat 901 acagagaaaa tattgcaagt ttgatggaca aatgttttcc tgagaaaaac aaaccaggtg 961 aaattgccaa atacatggaa acagtgaaat tactagacta cactgaaaaa cctctttatg 1021 aaaatttacg tgacattctt ttgcaaggac taaaagctat aggaagtaag gatgatggca 1081 aattggacct cagtgttgtg gagaatggag gtttgaaagc aaaaacaata acaaagaagc 1141 gaaagaaaga aattgaagaa agcaaggaac ctggtgttga agatacggaa tggtcaaaca 1201 cacagacaga ggaggccata cagacccgtt caagaaccag aaagagagtc cagaagttgc 1261 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1321 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1381 tta