Construct: ORF ccsbBroad304_07197
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014291.1_s304c1, BRDN0000400129
- Derived from:
- ccsbBroadEn_07197
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDHX (8050)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07197
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8050 | PDHX | pyruvate dehydrogenase comp... | NM_003477.3 | 99.8% | 99.8% | 63C>T;858T>C;1109A>T |
| 2 | human | 8050 | PDHX | pyruvate dehydrogenase comp... | NM_001135024.1 | 93.8% | 89.4% | (many diffs) |
| 3 | human | 8050 | PDHX | pyruvate dehydrogenase comp... | XM_011520390.1 | 87.8% | 87.8% | 0_1ins180;678T>C;929A>T |
| 4 | human | 8050 | PDHX | pyruvate dehydrogenase comp... | NM_001166158.1 | 54.5% | 54.4% | 63C>T;342_343ins681;428A>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1569
- ORF length:
- 1503
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcctcctgg aggctgggct gtgatccgcg gctgctgcgt tatcttgtgg 121 gcttccctgg ccgccgaagc gtagggctgg tgaagggggc tcttgggtgg tctgtaagcc 181 gcggagctaa ttggagatgg tttcacagca cgcagtggct tcggggtgat cccattaaga 241 tactaatgcc atcactgtct cctacaatgg aagaaggaaa cattgtgaaa tggctgaaaa 301 aggaaggtga agcggtgagt gctggagatg cattatgtga aattgagact gacaaagctg 361 tggttacctt agatgcaagt gatgatggaa tcttggccaa aatcgtggtt gaagaaggaa 421 gtaaaaatat acggctaggt tcactaattg gtttgatagt agaagaagga gaagattgga 481 aacatgttga aattcccaaa gacgtaggtc ctccaccacc agtttcaaaa ccttcagagc 541 ctcgcccctc accagaacca cagatttcca tccctgtcaa gaaggaacac atacccggga 601 cactacggtt ccgtttaagt ccagctgccc gcaatattct ggaaaaacac tcactggatg 661 ctagccaggg cacagccact ggccctcggg ggatattcac taaagaggat gctctcaaac 721 ttgtccagtt gaaacaaacg ggcaagatta ccgagtccag accaactcca gcccccacag 781 ccactcccac agcaccttcg cccctacagg ccacagctgg accatcttat ccccggcctg 841 tgatcccacc agtatcaact cctggacaac ccaatgcagt gggcacattc actgaaatcc 901 ccgccagcaa tattcgaaga gtcattgcca agagattaac tgaatctaaa agtactgtac 961 ctcatgcata tgctactgct gactgtgacc ttggagctgt tttaaaagtt aggcaagatc 1021 tggtcaaaga tgacattaaa gtatcagtaa atgattttat catcaaggca gcagctgtta 1081 cccttaaaca aatgccagat gttaatgtaa gctgggatgg agagggccca aagcaactgc 1141 catttattga catttcagtg gctgtggcaa cagttaaagg cttacttact ccaatcataa 1201 aagatgctgc tgctaaaggt atccaggaaa ttgctgactc tgtaaaggct ctatcaaaga 1261 aagcaagaga tggaaaattg ttgcctgaag aataccaagg aggatctttt agtatttcca 1321 acttggggat gtttggcatc gacgaattta ctgcagtgat taaccctcct caggcctgca 1381 ttttggcggt tgggaggttc cgacctgtgc tgaagctcac tgaggatgaa gagggaaatg 1441 ccaaactgca gcagcgccag ctcataacag tcacaatgtc aagtgacagt cgagtggttg 1501 atgacgaact ggcaaccagg tttcttaaaa gttttaaagc aaacctagag aatcctatcc 1561 gacttgccta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1621 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1681 tcaacctctg gatta