Construct: ORF ccsbBroad304_07220

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000919.1_s304c1, BRDN0000406835
Derived from:
ccsbBroadEn_07220
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CDC45 (8318)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07220
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8318 CDC45 cell division cycle 45 NM_003504.4 99.9% 100% 9G>A
2 human 8318 CDC45 cell division cycle 45 XM_024452277.1 99.7% 99.8% 9G>A;1560_1562delCAG
3 human 8318 CDC45 cell division cycle 45 NM_001369291.1 97.2% 96.9% (many diffs)
4 human 8318 CDC45 cell division cycle 45 XM_011530416.1 97.1% 96.8% (many diffs)
5 human 8318 CDC45 cell division cycle 45 NM_001178010.2 94.5% 94.6% 9G>A;541_636del
6 human 8318 CDC45 cell division cycle 45 NM_001178011.2 91.8% 91.8% 9G>A;201_202ins138
7 human 8318 CDC45 cell division cycle 45 XM_011530418.3 91.8% 91.8% 9G>A;201_202ins138
8 human 8318 CDC45 cell division cycle 45 XM_011530417.3 91.6% 91.7% 9G>A;201_202ins138;1422_1424delCAG
9 human 8318 CDC45 cell division cycle 45 NR_161281.1 78.4% (many diffs)
10 human 8318 CDC45 cell division cycle 45 XM_017028966.1 69.3% 68.2% (many diffs)
11 human 8318 CDC45 cell division cycle 45 XR_002958716.1 59.3% (many diffs)
12 human 8318 CDC45 cell division cycle 45 XM_017028967.1 58.3% 58.3% 0_1ins705;855_857delCAG
13 mouse 12544 Cdc45 cell division cycle 45 NM_009862.2 88.1% 91.6% (many diffs)
14 mouse 12544 Cdc45 cell division cycle 45 XM_006521744.2 88.1% 91.6% (many diffs)
15 mouse 12544 Cdc45 cell division cycle 45 XM_011245813.2 88.1% 91.6% (many diffs)
16 mouse 12544 Cdc45 cell division cycle 45 NM_001161623.1 81.3% 83.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1764
ORF length:
1698
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtt cgtatccgat ttccgcaaag agttctacga ggtggtccag agccagaggg
121tccttctctt cgtggcctcg gacgtggatg ctctgtgtgc gtgcaagatc cttcaggcct
181tgttccagtg tgaccacgtg caatatacgc tggttccagt ttctgggtgg caagaacttg
241aaactgcatt tcttgagcat aaagaacagt ttcattattt tattctcata aactgtggag
301ctaatgtaga cctattggat attcttcaac ctgatgaaga cactatattc tttgtgtgtg
361acacccatag gccagtcaat gtcgtcaatg tatacaacga tacccagatc aaattactca
421ttaaacaaga tgatgacctt gaagttcccg cctatgaaga catcttcagg gatgaagagg
481aggatgaaga gcattcagga aatgacagtg atgggtcaga gccttctgag aagcgcacac
541ggttagaaga ggagatagtg gagcaaacca tgcggaggag gcagcggcga gagtgggagg
601cccggagaag agacatcctc tttgactacg agcagtatga atatcatggg acatcgtcag
661ccatggtgat gtttgagctg gcttggatgc tgtccaagga cctgaatgac atgctgtggt
721gggccatcgt tggactaaca gaccagtggg tgcaagacaa gatcactcaa atgaaatacg
781tgactgatgt tggtgtcctg cagcgccacg tttcccgcca caaccaccgg aacgaggatg
841aggagaacac actctccgtg gactgcacac ggatctcctt tgagtatgac ctccgcctgg
901tgctctacca gcactggtcc ctccatgaca gcctgtgcaa caccagctat accgcagcca
961ggttcaagct gtggtctgtg catggacaga agcggctcca ggagttcctt gcagacatgg
1021gtcttcccct gaagcaggtg aagcagaagt tccaggccat ggacatctcc ttgaaggaga
1081atttgcggga aatgattgaa gagtctgcaa ataaatttgg gatgaaggac atgcgcgtgc
1141agactttcag cattcatttt gggttcaagc acaagtttct ggccagcgac gtggtctttg
1201ccaccatgtc tttgatggag agccccgaga aggatggctc agggacagat cacttcatcc
1261aggctctgga cagcctctcc aggagtaacc tggacaagct gtaccatggc ctggaactcg
1321ccaagaagca gctgcgagcc acccagcaga ccattgccag ctgcctttgc accaacctcg
1381tcatctccca ggggcctttc ctgtactgct ctctcatgga gggcactcca gatgtcatgc
1441tgttctctag gccggcatcc ctaagcctgc tcagcaaaca cctgctcaag tcctttgtgt
1501gttcgacaaa gaaccggcgc tgcaaactgc tgcccctggt gatggctgcc cccctgagca
1561tggagcatgg cacagtgacc gtggtgggca tccccccaga gaccgacagc tcggacagga
1621agaacttttt tgggagggcg tttgagaagg cagcggaaag caccagctcc cggatgctgc
1681acaaccattt tgacctctca gtaattgagc tgaaagctga ggatcggagc aagtttctgg
1741acgcacttat ttccctcctg tcctacccaa ctttcttgta caaagtggtt ggtaagccta
1801tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
1861gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)