Transcript: Human XM_011530418.3

PREDICTED: Homo sapiens cell division cycle 45 (CDC45), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CDC45 (8318)
Length:
2113
CDS:
422..1984

Additional Resources:

NCBI RefSeq record:
XM_011530418.3
NBCI Gene record:
CDC45 (8318)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011530418.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000433655 ATATACGCTGGTTCCAGTTTC pLKO_005 559 CDS 100% 10.800 15.120 N CDC45 n/a
2 TRCN0000145466 GCAGACTTTCAGCATTCATTT pLKO.1 1357 CDS 100% 13.200 9.240 N CDC45 n/a
3 TRCN0000145195 GCAAGAACTTGAAACTGCATT pLKO.1 586 CDS 100% 4.950 3.465 N CDC45 n/a
4 TRCN0000140824 GCAAGTTTCTGGACGCACTTA pLKO.1 1947 CDS 100% 4.950 3.465 N CDC45 n/a
5 TRCN0000173191 CCTCTCAGTAATTGAGCTGAA pLKO.1 1912 CDS 100% 4.050 2.835 N Cdc45 n/a
6 TRCN0000279261 CCTCTCAGTAATTGAGCTGAA pLKO_005 1912 CDS 100% 4.050 2.835 N Cdc45 n/a
7 TRCN0000139672 CGGAGAAGAGACATCCTCTTT pLKO.1 821 CDS 100% 0.495 0.347 N CDC45 n/a
8 TRCN0000193254 CAAGAACTTGAAACTGCATAT pLKO.1 587 CDS 100% 10.800 7.560 N Cdc45 n/a
9 TRCN0000176220 GCAAGAACTTGAAACTGCATA pLKO.1 586 CDS 100% 4.950 3.465 N Cdc45 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011530418.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07220 pDONR223 100% 91.8% 91.8% None 9G>A;201_202ins138 n/a
2 ccsbBroad304_07220 pLX_304 0% 91.8% 91.8% V5 9G>A;201_202ins138 n/a
3 TRCN0000477447 ATGAGCCGATCAGATTATCTGCCA pLX_317 17.6% 91.8% 91.8% V5 9G>A;201_202ins138 n/a
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