Transcript: Mouse NM_009862.2

Mus musculus cell division cycle 45 (Cdc45), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-14
Taxon:
Mus musculus (mouse)
Gene:
Cdc45 (12544)
Length:
2161
CDS:
314..2014

Additional Resources:

NCBI RefSeq record:
NM_009862.2
NBCI Gene record:
Cdc45 (12544)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_009862.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000279336 CAGTTTGGGTTCAAGCATAAA pLKO_005 1403 CDS 100% 13.200 18.480 N Cdc45 n/a
2 TRCN0000279341 GCGTCATGTGTCCCGTCATAA pLKO_005 1051 CDS 100% 13.200 18.480 N Cdc45 n/a
3 TRCN0000173191 CCTCTCAGTAATTGAGCTGAA pLKO.1 1942 CDS 100% 4.050 5.670 N Cdc45 n/a
4 TRCN0000279261 CCTCTCAGTAATTGAGCTGAA pLKO_005 1942 CDS 100% 4.050 5.670 N Cdc45 n/a
5 TRCN0000193315 CAATGACACTCAGATCAAATT pLKO.1 643 CDS 100% 13.200 9.240 N Cdc45 n/a
6 TRCN0000279345 CAATGACACTCAGATCAAATT pLKO_005 643 CDS 100% 13.200 9.240 N Cdc45 n/a
7 TRCN0000193254 CAAGAACTTGAAACTGCATAT pLKO.1 479 CDS 100% 10.800 7.560 N Cdc45 n/a
8 TRCN0000176220 GCAAGAACTTGAAACTGCATA pLKO.1 478 CDS 100% 4.950 3.465 N Cdc45 n/a
9 TRCN0000175306 CTTTGTGTACTCGACAAAGAA pLKO.1 1741 CDS 100% 0.563 0.394 N Cdc45 n/a
10 TRCN0000279263 CTTTGTGTACTCGACAAAGAA pLKO_005 1741 CDS 100% 0.563 0.394 N Cdc45 n/a
11 TRCN0000145195 GCAAGAACTTGAAACTGCATT pLKO.1 478 CDS 100% 4.950 3.465 N CDC45 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_009862.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07220 pDONR223 100% 88.1% 91.6% None (many diffs) n/a
2 ccsbBroad304_07220 pLX_304 0% 88.1% 91.6% V5 (many diffs) n/a
3 TRCN0000477447 ATGAGCCGATCAGATTATCTGCCA pLX_317 17.6% 88.1% 91.6% V5 (many diffs) n/a
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