Construct: ORF ccsbBroad304_07225
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005981.1_s304c1, BRDN0000460268
- Derived from:
- ccsbBroadEn_07225
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H3C11 (8354)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07225
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8354 | H3C11 | H3 clustered histone 11 | NM_003533.2 | 99.5% | 99.2% | 339T>C;365C>T |
| 2 | human | 8353 | H3C6 | H3 clustered histone 6 | NM_003532.2 | 87.7% | 99.2% | (many diffs) |
| 3 | human | 8351 | H3C4 | H3 clustered histone 4 | NM_003530.4 | 87.5% | 99.2% | (many diffs) |
| 4 | human | 8357 | H3C10 | H3 clustered histone 10 | NM_003536.2 | 87.5% | 99.2% | (many diffs) |
| 5 | human | 8968 | H3C7 | H3 clustered histone 7 | NM_021018.2 | 87% | 99.2% | (many diffs) |
| 6 | human | 333932 | H3C15 | H3 clustered histone 15 | NM_001005464.2 | 86.7% | 98.5% | (many diffs) |
| 7 | human | 126961 | H3C14 | H3 clustered histone 14 | NM_021059.2 | 86.7% | 98.5% | (many diffs) |
| 8 | human | 8290 | H3-4 | H3.4 histone | NM_003493.2 | 86.7% | 96.3% | (many diffs) |
| 9 | human | 8352 | H3C3 | H3 clustered histone 3 | NM_003531.2 | 86.5% | 99.2% | (many diffs) |
| 10 | human | 8355 | H3C8 | H3 clustered histone 8 | NM_003534.2 | 85.7% | 99.2% | (many diffs) |
| 11 | human | 8350 | H3C1 | H3 clustered histone 1 | NM_003529.2 | 85.5% | 99.2% | (many diffs) |
| 12 | human | 8358 | H3C2 | H3 clustered histone 2 | NM_003537.3 | 85.5% | 99.2% | (many diffs) |
| 13 | human | 653604 | H3C13 | H3 clustered histone 13 | NM_001123375.2 | 85.5% | 98.5% | (many diffs) |
| 14 | human | 8356 | H3C12 | H3 clustered histone 12 | NM_003535.2 | 84.8% | 99.2% | (many diffs) |
| 15 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001355409.3 | 84.5% | 96.3% | (many diffs) |
| 16 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001372105.1 | 84.5% | 96.3% | (many diffs) |
| 17 | human | 3021 | H3-3B | H3.3 histone B | NM_005324.5 | 79.1% | 95.5% | (many diffs) |
| 18 | human | 440093 | H3-5 | H3.5 histone | NM_001013699.3 | 76.9% | 92.6% | (many diffs) |
| 19 | human | 3020 | H3-3A | H3.3 histone A | NM_002107.6 | 75% | 95.5% | (many diffs) |
| 20 | mouse | 319152 | Hist1h3h | histone cluster 1, H3h | NM_178206.2 | 88.4% | 99.2% | (many diffs) |
| 21 | mouse | 319148 | Hist1h3c | histone cluster 1, H3c | NM_175653.2 | 88.2% | 98.5% | (many diffs) |
| 22 | mouse | 319150 | Hist1h3b | histone cluster 1, H3b | NM_178203.2 | 88.2% | 98.5% | (many diffs) |
| 23 | mouse | 319153 | Hist1h3i | histone cluster 1, H3i | NM_178207.2 | 87.9% | 99.2% | (many diffs) |
| 24 | mouse | 360198 | Hist1h3a | histone cluster 1, H3a | NM_013550.5 | 87.7% | 99.2% | (many diffs) |
| 25 | mouse | 97908 | Hist1h3g | histone cluster 1, H3g | NM_145073.2 | 87.7% | 99.2% | (many diffs) |
| 26 | mouse | 97114 | Hist2h3c2 | histone cluster 2, H3c2 | NM_054045.4 | 87.7% | 98.5% | (many diffs) |
| 27 | mouse | 319149 | Hist1h3d | histone cluster 1, H3d | NM_178204.2 | 87.7% | 98.5% | (many diffs) |
| 28 | mouse | 319154 | Hist2h3b | histone cluster 2, H3b | NM_178215.2 | 87.7% | 98.5% | (many diffs) |
| 29 | mouse | 15077 | Hist2h3c1 | histone cluster 2, H3c1 | NM_178216.3 | 87.7% | 98.5% | (many diffs) |
| 30 | mouse | 319151 | Hist1h3e | histone cluster 1, H3e | NM_178205.2 | 87.5% | 98.5% | (many diffs) |
| 31 | mouse | 260423 | Hist1h3f | histone cluster 1, H3f | NM_013548.4 | 87.2% | 98.5% | (many diffs) |
| 32 | mouse | 382523 | Gm12260 | predicted gene 12260 | NM_001318003.2 | 84.3% | 97% | (many diffs) |
| 33 | mouse | 15081 | H3f3b | H3 histone, family 3B | NM_008211.3 | 79.1% | 95.5% | (many diffs) |
| 34 | mouse | 15081 | H3f3b | H3 histone, family 3B | XM_006532248.2 | 79.1% | 95.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 477
- ORF length:
- 408
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcacgaaca aagcaaacag ctcgcaagtc caccggcggc aaagcgccgc 121 gcaagcagct ggccaccaag gcggctcgca agagcgctcc ggccaccggt ggcgtcaaga 181 agccccaccg ctaccgcccc ggcaccgtgg ccctgcgcga gatccgccgc taccagaagt 241 cgaccgagct gctaatccgg aagctacctt ttcagcgctt ggtacgggag atcgcacagg 301 actttaagac cgatctgcgc ttccagagct cggcggtgat ggcgctgcag gaggcttgcg 361 aggcctacct ggtggggcta tttgaggata ccaacctgtg cgccatccac gccaaacgcg 421 tcactattat gcttaaagac atccagcttg cgcgccgcat ccgaggggag agggcattgc 481 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 541 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 601 tta