Construct: ORF ccsbBroad304_07252
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007203.1_s304c1, BRDN0000461789
- Derived from:
- ccsbBroadEn_07252
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DGKZ (8525)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07252
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8525 | DGKZ | diacylglycerol kinase zeta | NM_003646.4 | 99.9% | 99.8% | 174C>G;1570C>A |
| 2 | human | 8525 | DGKZ | diacylglycerol kinase zeta | NM_001199267.2 | 99.8% | 99.7% | 174C>G;365_366insGCA;1567C>A |
| 3 | human | 8525 | DGKZ | diacylglycerol kinase zeta | NM_001199266.2 | 99.3% | 99.3% | 174C>G;1570C>A;2574_2588del |
| 4 | human | 8525 | DGKZ | diacylglycerol kinase zeta | NM_001199268.2 | 97.4% | 97.4% | 174C>G;499_500ins69;1501C>A |
| 5 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448737.1 | 96.5% | 96.4% | 174C>G;1524_1622del;1669C>A |
| 6 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448738.1 | 96.3% | 96.3% | (many diffs) |
| 7 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_005253182.3 | 96.1% | 93% | (many diffs) |
| 8 | human | 8525 | DGKZ | diacylglycerol kinase zeta | NM_201533.3 | 96% | 92.9% | (many diffs) |
| 9 | human | 8525 | DGKZ | diacylglycerol kinase zeta | NM_201532.3 | 95.1% | 92.6% | (many diffs) |
| 10 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448735.1 | 92.9% | 89.9% | (many diffs) |
| 11 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448736.1 | 92.8% | 89.8% | (many diffs) |
| 12 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448733.1 | 92% | 89.6% | (many diffs) |
| 13 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448734.1 | 91.9% | 89.5% | (many diffs) |
| 14 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448739.1 | 90.5% | 87.6% | (many diffs) |
| 15 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448741.1 | 84% | 83.9% | 0_1ins360;1164_1262del;1309C>A |
| 16 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448740.1 | 83.7% | 76.3% | (many diffs) |
| 17 | human | 8525 | DGKZ | diacylglycerol kinase zeta | NM_001105540.2 | 81.7% | 78.7% | (many diffs) |
| 18 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448732.1 | 79.5% | 76.5% | (many diffs) |
| 19 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_017018455.1 | 78.9% | 78.8% | 162_902del;915C>G;2311C>A |
| 20 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_017018456.1 | 78.8% | 78.7% | (many diffs) |
| 21 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448729.1 | 76.7% | 76.6% | (many diffs) |
| 22 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448730.1 | 76.7% | 76.5% | (many diffs) |
| 23 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XM_024448731.1 | 74.8% | 74.7% | (many diffs) |
| 24 | human | 8525 | DGKZ | diacylglycerol kinase zeta | XR_002957206.1 | 62.6% | (many diffs) | |
| 25 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | NM_138306.2 | 88.5% | 95.4% | (many diffs) |
| 26 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_006498542.3 | 87.3% | 94.1% | (many diffs) |
| 27 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_006498543.2 | 85.5% | 88.8% | (many diffs) |
| 28 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_006498541.2 | 84.5% | 89.9% | (many diffs) |
| 29 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_006498540.2 | 84.4% | 87.6% | (many diffs) |
| 30 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_006498539.1 | 83.5% | 88.7% | (many diffs) |
| 31 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_017314875.1 | 75.9% | 82.3% | (many diffs) |
| 32 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_017314878.1 | 75.9% | 82.3% | (many diffs) |
| 33 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | NM_001166597.1 | 72% | 74.7% | (many diffs) |
| 34 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_017314867.1 | 71.9% | 74.7% | (many diffs) |
| 35 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_006498538.2 | 71.2% | 73.8% | (many diffs) |
| 36 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_006498537.1 | 71.2% | 73.8% | (many diffs) |
| 37 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_011239240.1 | 61.5% | 63.1% | (many diffs) |
| 38 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_011239241.2 | 41.4% | 41.4% | (many diffs) |
| 39 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XM_011239244.1 | 38% | 30.2% | (many diffs) |
| 40 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XR_374381.2 | 29% | (many diffs) | |
| 41 | mouse | 104418 | Dgkz | diacylglycerol kinase zeta | XR_866203.1 | 23.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2853
- ORF length:
- 2787
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gccgcgggac ggtagccccg aggcccggag cagcgactcc gagtcggctt 121 ccgcctcgtc cagcggctcc gagcgcgacg ccggtcccga gccggacaag gcgccgcggc 181 gactcaacaa gcggcgcttc ccggggctgc ggctcttcgg gcacaggaaa gccatcacga 241 agtcgggcct ccagcacctg gccccccctc cgcccacccc tggggccccg tgcagcgagt 301 cagagcggca gatccggagt acagtggact ggagcgagtc agcgacatat ggggagcaca 361 tctggttcga gaccaacgtg tccggggact tctgctacgt tggggagcag tactgtgtag 421 ccaggatgct gcagaagtca gtgtctcgaa gaaagtgcgc agcctgcaag attgtggtgc 481 acacgccctg catcgagcag ctggagaaga taaatttccg ctgtaagccg tccttccgtg 541 aatcaggctc caggaatgtc cgcgagccaa cctttgtacg gcaccactgg gtacacagac 601 gacgccagga cggcaagtgt cggcactgtg ggaagggatt ccagcagaag ttcaccttcc 661 acagcaagga gattgtggcc atcagctgct cgtggtgcaa gcaggcatac cacagcaagg 721 tgtcctgctt catgctgcag cagatcgagg agccgtgctc gctgggggtc cacgcagccg 781 tggtcatccc gcccacctgg atcctccgcg cccggaggcc ccagaatact ctgaaagcaa 841 gcaagaagaa gaagagggca tccttcaaga ggaagtccag caagaaaggg cctgaggagg 901 gccgctggag acccttcatc atcaggccca ccccctcccc gctcatgaag cccctgctgg 961 tgtttgtgaa ccccaagagt gggggcaacc agggtgcaaa gatcatccag tctttcctct 1021 ggtatctcaa tccccgacaa gtcttcgacc tgagccaggg agggcccaag gaggcgctgg 1081 agatgtaccg caaagtgcac aacctgcgga tcctggcgtg cgggggcgac ggcacggtgg 1141 gctggatcct ctccaccctg gaccagctac gcctgaagcc gccaccccct gttgccatcc 1201 tgcccctggg tactggcaac gacttggccc gaaccctcaa ctggggtggg ggctacacag 1261 atgagcctgt gtccaagatc ctctcccacg tggaggaggg gaacgtggta cagctggacc 1321 gctgggacct ccacgctgag cccaaccccg aggcagggcc tgaggaccga gatgaaggcg 1381 ccaccgaccg gttgcccctg gatgtcttca acaactactt cagcctgggc tttgacgccc 1441 acgtcaccct ggagttccac gagtctcgag aggccaaccc agagaaattc aacagccgct 1501 ttcggaataa gatgttctac gccgggacag ctttctctga cttcctgatg ggcagctcca 1561 aggacctggc caagcacatc cgagtggtgt gtgatggaat ggacttgact cccaagatcc 1621 aggacctgaa acccaagtgt gttgttttcc tgaacatccc caggtactgt gcgggcacca 1681 tgccctgggg ccaccctggg gagcaccacg actttgagcc ccagcggcat gacgacggct 1741 acctcgaggt cattggcttc accatgacgt cgttggccgc gctgcaggtg ggcggacacg 1801 gcgagcggct gacgcagtgt cgcgaggtgg tgctcaccac atccaaggcc atcccggtgc 1861 aggtggatgg cgagccctgc aagcttgcag cctcacgcat ccgcatcgcc ctgcgcaacc 1921 aggccaccat ggtgcagaag gccaagcggc ggagcgccgc ccccctgcac agcgaccagc 1981 agccggtgcc agagcagttg cgcatccagg tgagtcgcgt cagcatgcac gactatgagg 2041 ccctgcacta cgacaaggag cagctcaagg aggcctctgt gccgctgggc actgtggtgg 2101 tcccaggaga cagtgaccta gagctctgcc gtgcccacat tgagagactc cagcaggagc 2161 ccgatggtgc tggagccaag tccccgacat gccagaaact gtcccccaag tggtgcttcc 2221 tggacgccac cactgccagc cgcttctaca ggatcgaccg agcccaggag cacctcaact 2281 atgtgactga gatcgcacag gatgagattt atatcctgga ccctgagctg ctgggggcat 2341 cggcccggcc tgacctccca acccccactt cccctctccc cacctcaccc tgctcaccca 2401 cgccccggtc actgcaaggg gatgctgcac cccctcaagg tgaagagctg attgaggctg 2461 ccaagaggaa cgacttctgt aagctccagg agctgcaccg agctgggggc gacctcatgc 2521 accgagacga gcagagtcgc acgctcctgc accacgcagt cagcactggc agcaaggatg 2581 tggtccgcta cctgctggac cacgcccccc cagagatcct tgatgcggtg gaggaaaacg 2641 gggagacctg tttgcaccaa gcagcggccc tgggccagcg caccatctgc cactacatcg 2701 tggaggccgg ggcctcgctc atgaagacag accagcaggg cgacactccc cggcagcggg 2761 ctgagaaggc tcaggacacc gagctggccg cctacctgga gaaccggcag cactaccaga 2821 tgatccagcg ggaggaccag gagacggctg tgtacccaac tttcttgtac aaagtggttg 2881 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 2941 accggtggcg cgttaagtcg acaatcaacc tctggatta