Construct: ORF ccsbBroad304_07252

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007203.1_s304c1, BRDN0000461789
Derived from:
ccsbBroadEn_07252
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DGKZ (8525)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07252
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8525 DGKZ diacylglycerol kinase zeta NM_003646.4 99.9% 99.8% 174C>G;1570C>A
2 human 8525 DGKZ diacylglycerol kinase zeta NM_001199267.2 99.8% 99.7% 174C>G;365_366insGCA;1567C>A
3 human 8525 DGKZ diacylglycerol kinase zeta NM_001199266.2 99.3% 99.3% 174C>G;1570C>A;2574_2588del
4 human 8525 DGKZ diacylglycerol kinase zeta NM_001199268.2 97.4% 97.4% 174C>G;499_500ins69;1501C>A
5 human 8525 DGKZ diacylglycerol kinase zeta XM_024448737.1 96.5% 96.4% 174C>G;1524_1622del;1669C>A
6 human 8525 DGKZ diacylglycerol kinase zeta XM_024448738.1 96.3% 96.3% (many diffs)
7 human 8525 DGKZ diacylglycerol kinase zeta XM_005253182.3 96.1% 93% (many diffs)
8 human 8525 DGKZ diacylglycerol kinase zeta NM_201533.3 96% 92.9% (many diffs)
9 human 8525 DGKZ diacylglycerol kinase zeta NM_201532.3 95.1% 92.6% (many diffs)
10 human 8525 DGKZ diacylglycerol kinase zeta XM_024448735.1 92.9% 89.9% (many diffs)
11 human 8525 DGKZ diacylglycerol kinase zeta XM_024448736.1 92.8% 89.8% (many diffs)
12 human 8525 DGKZ diacylglycerol kinase zeta XM_024448733.1 92% 89.6% (many diffs)
13 human 8525 DGKZ diacylglycerol kinase zeta XM_024448734.1 91.9% 89.5% (many diffs)
14 human 8525 DGKZ diacylglycerol kinase zeta XM_024448739.1 90.5% 87.6% (many diffs)
15 human 8525 DGKZ diacylglycerol kinase zeta XM_024448741.1 84% 83.9% 0_1ins360;1164_1262del;1309C>A
16 human 8525 DGKZ diacylglycerol kinase zeta XM_024448740.1 83.7% 76.3% (many diffs)
17 human 8525 DGKZ diacylglycerol kinase zeta NM_001105540.2 81.7% 78.7% (many diffs)
18 human 8525 DGKZ diacylglycerol kinase zeta XM_024448732.1 79.5% 76.5% (many diffs)
19 human 8525 DGKZ diacylglycerol kinase zeta XM_017018455.1 78.9% 78.8% 162_902del;915C>G;2311C>A
20 human 8525 DGKZ diacylglycerol kinase zeta XM_017018456.1 78.8% 78.7% (many diffs)
21 human 8525 DGKZ diacylglycerol kinase zeta XM_024448729.1 76.7% 76.6% (many diffs)
22 human 8525 DGKZ diacylglycerol kinase zeta XM_024448730.1 76.7% 76.5% (many diffs)
23 human 8525 DGKZ diacylglycerol kinase zeta XM_024448731.1 74.8% 74.7% (many diffs)
24 human 8525 DGKZ diacylglycerol kinase zeta XR_002957206.1 62.6% (many diffs)
25 mouse 104418 Dgkz diacylglycerol kinase zeta NM_138306.2 88.5% 95.4% (many diffs)
26 mouse 104418 Dgkz diacylglycerol kinase zeta XM_006498542.3 87.3% 94.1% (many diffs)
27 mouse 104418 Dgkz diacylglycerol kinase zeta XM_006498543.2 85.5% 88.8% (many diffs)
28 mouse 104418 Dgkz diacylglycerol kinase zeta XM_006498541.2 84.5% 89.9% (many diffs)
29 mouse 104418 Dgkz diacylglycerol kinase zeta XM_006498540.2 84.4% 87.6% (many diffs)
30 mouse 104418 Dgkz diacylglycerol kinase zeta XM_006498539.1 83.5% 88.7% (many diffs)
31 mouse 104418 Dgkz diacylglycerol kinase zeta XM_017314875.1 75.9% 82.3% (many diffs)
32 mouse 104418 Dgkz diacylglycerol kinase zeta XM_017314878.1 75.9% 82.3% (many diffs)
33 mouse 104418 Dgkz diacylglycerol kinase zeta NM_001166597.1 72% 74.7% (many diffs)
34 mouse 104418 Dgkz diacylglycerol kinase zeta XM_017314867.1 71.9% 74.7% (many diffs)
35 mouse 104418 Dgkz diacylglycerol kinase zeta XM_006498538.2 71.2% 73.8% (many diffs)
36 mouse 104418 Dgkz diacylglycerol kinase zeta XM_006498537.1 71.2% 73.8% (many diffs)
37 mouse 104418 Dgkz diacylglycerol kinase zeta XM_011239240.1 61.5% 63.1% (many diffs)
38 mouse 104418 Dgkz diacylglycerol kinase zeta XM_011239241.2 41.4% 41.4% (many diffs)
39 mouse 104418 Dgkz diacylglycerol kinase zeta XM_011239244.1 38% 30.2% (many diffs)
40 mouse 104418 Dgkz diacylglycerol kinase zeta XR_374381.2 29% (many diffs)
41 mouse 104418 Dgkz diacylglycerol kinase zeta XR_866203.1 23.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2853
ORF length:
2787
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gccgcgggac ggtagccccg aggcccggag cagcgactcc gagtcggctt
121ccgcctcgtc cagcggctcc gagcgcgacg ccggtcccga gccggacaag gcgccgcggc
181gactcaacaa gcggcgcttc ccggggctgc ggctcttcgg gcacaggaaa gccatcacga
241agtcgggcct ccagcacctg gccccccctc cgcccacccc tggggccccg tgcagcgagt
301cagagcggca gatccggagt acagtggact ggagcgagtc agcgacatat ggggagcaca
361tctggttcga gaccaacgtg tccggggact tctgctacgt tggggagcag tactgtgtag
421ccaggatgct gcagaagtca gtgtctcgaa gaaagtgcgc agcctgcaag attgtggtgc
481acacgccctg catcgagcag ctggagaaga taaatttccg ctgtaagccg tccttccgtg
541aatcaggctc caggaatgtc cgcgagccaa cctttgtacg gcaccactgg gtacacagac
601gacgccagga cggcaagtgt cggcactgtg ggaagggatt ccagcagaag ttcaccttcc
661acagcaagga gattgtggcc atcagctgct cgtggtgcaa gcaggcatac cacagcaagg
721tgtcctgctt catgctgcag cagatcgagg agccgtgctc gctgggggtc cacgcagccg
781tggtcatccc gcccacctgg atcctccgcg cccggaggcc ccagaatact ctgaaagcaa
841gcaagaagaa gaagagggca tccttcaaga ggaagtccag caagaaaggg cctgaggagg
901gccgctggag acccttcatc atcaggccca ccccctcccc gctcatgaag cccctgctgg
961tgtttgtgaa ccccaagagt gggggcaacc agggtgcaaa gatcatccag tctttcctct
1021ggtatctcaa tccccgacaa gtcttcgacc tgagccaggg agggcccaag gaggcgctgg
1081agatgtaccg caaagtgcac aacctgcgga tcctggcgtg cgggggcgac ggcacggtgg
1141gctggatcct ctccaccctg gaccagctac gcctgaagcc gccaccccct gttgccatcc
1201tgcccctggg tactggcaac gacttggccc gaaccctcaa ctggggtggg ggctacacag
1261atgagcctgt gtccaagatc ctctcccacg tggaggaggg gaacgtggta cagctggacc
1321gctgggacct ccacgctgag cccaaccccg aggcagggcc tgaggaccga gatgaaggcg
1381ccaccgaccg gttgcccctg gatgtcttca acaactactt cagcctgggc tttgacgccc
1441acgtcaccct ggagttccac gagtctcgag aggccaaccc agagaaattc aacagccgct
1501ttcggaataa gatgttctac gccgggacag ctttctctga cttcctgatg ggcagctcca
1561aggacctggc caagcacatc cgagtggtgt gtgatggaat ggacttgact cccaagatcc
1621aggacctgaa acccaagtgt gttgttttcc tgaacatccc caggtactgt gcgggcacca
1681tgccctgggg ccaccctggg gagcaccacg actttgagcc ccagcggcat gacgacggct
1741acctcgaggt cattggcttc accatgacgt cgttggccgc gctgcaggtg ggcggacacg
1801gcgagcggct gacgcagtgt cgcgaggtgg tgctcaccac atccaaggcc atcccggtgc
1861aggtggatgg cgagccctgc aagcttgcag cctcacgcat ccgcatcgcc ctgcgcaacc
1921aggccaccat ggtgcagaag gccaagcggc ggagcgccgc ccccctgcac agcgaccagc
1981agccggtgcc agagcagttg cgcatccagg tgagtcgcgt cagcatgcac gactatgagg
2041ccctgcacta cgacaaggag cagctcaagg aggcctctgt gccgctgggc actgtggtgg
2101tcccaggaga cagtgaccta gagctctgcc gtgcccacat tgagagactc cagcaggagc
2161ccgatggtgc tggagccaag tccccgacat gccagaaact gtcccccaag tggtgcttcc
2221tggacgccac cactgccagc cgcttctaca ggatcgaccg agcccaggag cacctcaact
2281atgtgactga gatcgcacag gatgagattt atatcctgga ccctgagctg ctgggggcat
2341cggcccggcc tgacctccca acccccactt cccctctccc cacctcaccc tgctcaccca
2401cgccccggtc actgcaaggg gatgctgcac cccctcaagg tgaagagctg attgaggctg
2461ccaagaggaa cgacttctgt aagctccagg agctgcaccg agctgggggc gacctcatgc
2521accgagacga gcagagtcgc acgctcctgc accacgcagt cagcactggc agcaaggatg
2581tggtccgcta cctgctggac cacgcccccc cagagatcct tgatgcggtg gaggaaaacg
2641gggagacctg tttgcaccaa gcagcggccc tgggccagcg caccatctgc cactacatcg
2701tggaggccgg ggcctcgctc atgaagacag accagcaggg cgacactccc cggcagcggg
2761ctgagaaggc tcaggacacc gagctggccg cctacctgga gaaccggcag cactaccaga
2821tgatccagcg ggaggaccag gagacggctg tgtacccaac tttcttgtac aaagtggttg
2881gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
2941accggtggcg cgttaagtcg acaatcaacc tctggatta

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