Transcript: Mouse XM_006498542.3

PREDICTED: Mus musculus diacylglycerol kinase zeta (Dgkz), transcript variant X9, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Dgkz (104418)
Length:
3700
CDS:
283..3114

Additional Resources:

NCBI RefSeq record:
XM_006498542.3
NBCI Gene record:
Dgkz (104418)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006498542.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000000670 ACAGGAAAGCCATCACCAAGT pLKO.1 440 CDS 100% 4.050 5.670 N DGKZ n/a
2 TRCN0000025394 CCCAAGATTCAGGACCTGAAA pLKO.1 1828 CDS 100% 4.950 3.465 N Dgkz n/a
3 TRCN0000297512 CCCAAGATTCAGGACCTGAAA pLKO_005 1828 CDS 100% 4.950 3.465 N Dgkz n/a
4 TRCN0000025396 CCTGGATGTCTTTAACAACTA pLKO.1 1614 CDS 100% 4.950 3.465 N Dgkz n/a
5 TRCN0000278613 CCTGGATGTCTTTAACAACTA pLKO_005 1614 CDS 100% 4.950 3.465 N Dgkz n/a
6 TRCN0000025395 CCTGTAAGATCGTGGTGCATA pLKO.1 680 CDS 100% 4.950 3.465 N Dgkz n/a
7 TRCN0000278614 CCTGTAAGATCGTGGTGCATA pLKO_005 680 CDS 100% 4.950 3.465 N Dgkz n/a
8 TRCN0000025397 CGAGGCTCTACATTATGACAA pLKO.1 2253 CDS 100% 4.950 3.465 N Dgkz n/a
9 TRCN0000278682 CGAGGCTCTACATTATGACAA pLKO_005 2253 CDS 100% 4.950 3.465 N Dgkz n/a
10 TRCN0000196402 GCTTTCGGAATAAGATGTTCT pLKO.1 1715 CDS 100% 4.950 3.465 N DGKZ n/a
11 TRCN0000025398 GAGAAGTTCAACAGCCGCTTT pLKO.1 1699 CDS 100% 4.050 2.835 N Dgkz n/a
12 TRCN0000278690 GAGAAGTTCAACAGCCGCTTT pLKO_005 1699 CDS 100% 4.050 2.835 N Dgkz n/a
13 TRCN0000199672 GCGCACCATCTGCCACTACAT pLKO.1 2937 CDS 100% 1.650 1.155 N DGKZ n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006498542.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07252 pDONR223 100% 87.3% 94.1% None (many diffs) n/a
2 ccsbBroad304_07252 pLX_304 0% 87.3% 94.1% V5 (many diffs) n/a
3 TRCN0000477876 CTTCAGCCTTCAAATAGGAGTGCC pLX_317 11.2% 87.3% 94.1% V5 (many diffs) n/a
4 ccsbBroadEn_14900 pDONR223 58.3% 85.7% 26.6% None (many diffs) n/a
5 ccsbBroad304_14900 pLX_304 0% 85.7% 26.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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