Transcript: Human NM_001199268.2

Homo sapiens diacylglycerol kinase zeta (DGKZ), transcript variant 7, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
DGKZ (8525)
Length:
3522
CDS:
198..2918

Additional Resources:

NCBI RefSeq record:
NM_001199268.2
NBCI Gene record:
DGKZ (8525)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147025 GAATAAGATGTTCTACGCCG pXPR_003 GGG 1387 51% 15 0.5152 DGKZ DGKZ 77466
2 BRDN0001149311 CTCTCCTCAGTACCACAGCA pXPR_003 AGG 580 21% 7 0.2077 DGKZ DGKZ 77465
3 BRDN0001147837 CCCCGTGCAGCGAGTCAGAG pXPR_003 CGG 237 9% 2 -0.3157 DGKZ DGKZ 77464
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001199268.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000000668 TCGCACAGGATGAGATTTATA pLKO.1 2356 CDS 100% 15.000 21.000 N DGKZ n/a
2 TRCN0000000670 ACAGGAAAGCCATCACCAAGT pLKO.1 355 CDS 100% 4.050 5.670 N DGKZ n/a
3 TRCN0000199594 GCGCTGGAGATGTACCGCAAA pLKO.1 1137 CDS 100% 1.350 1.080 N DGKZ n/a
4 TRCN0000010535 AGATGAGCCTGTGTCCAAGAT pLKO.1 1322 CDS 100% 4.950 3.465 N DGKZ n/a
5 TRCN0000194899 CCTCAACTATGTGACTGAGAT pLKO.1 2336 CDS 100% 4.950 3.465 N DGKZ n/a
6 TRCN0000196343 GAAGATAAATTTCCGCTGTAA pLKO.1 638 CDS 100% 4.950 3.465 N DGKZ n/a
7 TRCN0000196402 GCTTTCGGAATAAGATGTTCT pLKO.1 1561 CDS 100% 4.950 3.465 N DGKZ n/a
8 TRCN0000010536 AGTGGTGTGTGATGGAATGGA pLKO.1 1646 CDS 100% 3.000 2.100 N DGKZ n/a
9 TRCN0000000669 TGTGATGGAATGGACTTGACT pLKO.1 1653 CDS 100% 3.000 2.100 N DGKZ n/a
10 TRCN0000199672 GCGCACCATCTGCCACTACAT pLKO.1 2741 CDS 100% 1.650 1.155 N DGKZ n/a
11 TRCN0000199918 GCCTCGCTCATGAAGACAGAC pLKO.1 2775 CDS 100% 1.350 0.945 N DGKZ n/a
12 TRCN0000199030 CCGGGCTTCCTCCCGGAAACT pLKO.1 3333 3UTR 100% 0.000 0.000 N DGKZ n/a
13 TRCN0000199197 CCCACCTCACTGCCACATTCC pLKO.1 2977 3UTR 100% 0.000 0.000 N DGKZ n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001199268.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07252 pDONR223 100% 97.4% 97.4% None 174C>G;499_500ins69;1501C>A n/a
2 ccsbBroad304_07252 pLX_304 0% 97.4% 97.4% V5 174C>G;499_500ins69;1501C>A n/a
3 TRCN0000477876 CTTCAGCCTTCAAATAGGAGTGCC pLX_317 11.2% 97.4% 97.4% V5 174C>G;499_500ins69;1501C>A n/a
4 ccsbBroadEn_14900 pDONR223 58.3% 95.3% 26.1% None (many diffs) n/a
5 ccsbBroad304_14900 pLX_304 0% 95.3% 26.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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